NFYB9 Antibody

Code CSB-PA882844XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NFYB9 Polyclonal antibody
Uniprot No.
Target Names
NFYB9
Alternative Names
NFYB9 antibody; LEC1 antibody; At1g21970 antibody; T26F17.20 antibody; Nuclear transcription factor Y subunit B-9 antibody; AtNF-YB-9 antibody; Protein LEAFY COTYLEDON 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NFYB9 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the NF-Y/HAP transcription factor complex. The NF-Y complex stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters. Acts as a central regulator of the embryogenesis. Required for the speciation of cotyledon identity and the completion of embryo maturation. Controls seed storage protein genes through the regulation of FUS3 and ABI3. Involved in the blue light (BL) and abscisic acid (ABA) signaling pathways.
Gene References into Functions
  1. the mutations of key amino acids for the function of LEC1 in planta (D86K) prevented the interaction with LEC2. These results provide molecular evidences for the binding of LEC1 to B2-domain containing transcription factors, to form heteromers, involved in the control of gene expression. PMID: 29580949
  2. results show that LEC1 controls distinct gene sets at different developmental stages, including those that mediate the temporal transition between photosynthesis and chloroplast biogenesis early in seed development and seed maturation late in development. PMID: 28739919
  3. the seed-specific transcription factor LEAFY COTYLEDON1 promotes the initial establishment of an active chromatin state at FLC and activates its expression de novo in the pro-embryo, thus reversing the silenced state inherited from gametes; this active chromatin state is passed on from the pro-embryo to post-embryonic life, and leads to transmission of the embryonic memory of FLC activation to post-embryonic stages PMID: 29072296
  4. NUCLEAR FACTOR Y (NF-Y) binds a CCAAT box in the distal enhancer element and that CCAAT disruption dramatically reduces FT promoter activity PMID: 24610724
  5. In addition to VAL B3 factors, epigenetic mechanisms are implicated in maintaining repression of LEC1/AFL (LAFL)network during vegetative development PMID: 24902838
  6. Data confirms LEC1's known participation in the regulation of somatic embryogenesis, but also indicates additional roles in embryonic and extra-embryonic cell elongation PMID: 23073004
  7. NUCLEAR FACTOR Y transcription factors have both opposing and additive roles in abscisic acid-mediated seed germination. PMID: 23527203
  8. LEC1 appears to be an integrator of various regulatory events, involving the transcription factor itself as well as light and hormone signalling, especially during somatic and early zygotic embryogenesis. PMID: 22429691
  9. Cultures of the lec mutants formed somatic embryos at a low frequency. Moreover, somatic embryos were formed from callus tissue through an indirect route. PMID: 16034595
  10. A study of the network of transcription factors that regulates gene expression in A. thaliana is described and discussed. PMID: 17158584
  11. LEC1 positively regulates fatty acid biosynthesis genes. Genes involved in glycolysis and lipid accumulation are also up-regulated. PMID: 18689444
  12. LEC1 and LEC1-LIKE activate the CRC and SUCROSE SYNTHASE 2 (SUS2) promoters in combination with an NF-YC subunit through the interaction with a seed-specific ABA-response element (ABRE) binding bZIP factor, bZIP67. PMID: 19207209

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Subcellular Location
Nucleus.
Protein Families
NFYB/HAP3 subunit family
Tissue Specificity
Expressed in green siliques. Present in etiolated seedlings.
Database Links

KEGG: ath:AT1G21970

STRING: 3702.AT1G21970.1

UniGene: At.28011

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