Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRPD2 Polyclonal antibody

Uniprot No.

Q9LK40

Target Names

NRPD2

Alternative Names

NRPD2 antibody; DMS2 antibody; DRD2 antibody; NRPD2a antibody; NRPE2 antibody; RPD2a antibody; At3g23780 antibody; MYM9.13 antibody; DNA-directed RNA polymerases IV and V subunit 2 antibody; EC 2.7.7.6 antibody; DNA-directed RNA polymerase D subunit 2a antibody; AtNRPD2a antibody; Nuclear RNA polymerase D 2a antibody; Nuclear RNA polymerase E 2 antibody; Protein DEFECTIVE IN MERISTEM SILENCING 2 antibody; Protein DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 2 antibody; RNA polymerase IV subunit 2a antibody; POL IV 2a antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana NRPD2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Also required for full erasure of methylation when the RNA trigger is withdrawn. Required for intercellular RNA interference (RNAi) leading to systemic post-transcriptional gene silencing. Involved in the maintenance of post-transcriptional RNA silencing. During interphase, mediates siRNA-independent heterochromatin association and methylation into chromocenters and condensation and cytosine methylation at pericentromeric major repeats. Required for complete maintenance of the 35S promoter homology-dependent TGS in transgenic plants and for the initial establishment of DNA methylation.

Gene References into Functions

DRD1-Pol V-dependent de novo methylation may play a role in restraining the inappropriate silencing of active protein-coding genes in plants. [NRPD2] PMID: 21771120
Data demonstrate that Pol V functions independently of Pol IV, RDR2, and DCL3-mediated siRNA production to affect interphase heterochromatin organization, possibly by involving RNAs that recruit structural or chromatin-modifying proteins. PMID: 19825650
RNA Pol IV helps produce siRNAs that target de novo cytosine methylation events required for facultative heterochromatin formation and higher-order heterochromatin associations. PMID: 15766525
Results show that RNA polymerase IV (Pol IV, subunits NRPD1a and NRPD2) silences certain transposons and repetitive DNA in a short interfering RNA pathway involving RNA-dependent RNA polymerase 2 and Dicer-like 3(DCL3) [NRPD2] PMID: 15692015

Subcellular Location

Nucleus. Note=Concentrated in numerous distinct foci around chromocenters.

Protein Families

RNA polymerase beta chain family

Tissue Specificity

Mostly expressed in seedlings, flowers and roots, present ubiquitously, except in sperm cells.

Database Links

KEGG: ath:AT3G23780

STRING: 3702.AT3G23780.1

UniGene: At.37653

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NRPD2 Antibody

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