Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) pAbp Polyclonal antibody

Uniprot No.

P21187

Target Names

pAbp

Alternative Names

pAbp antibody; CG5119Polyadenylate-binding protein antibody; PABP antibody; Poly(A)-binding protein antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) pAbp protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Binds the poly(A) tail of mRNA. Since it interacts with the cap-associating translation initiation factor eIF4G, it is likely that it functions by linking Atx2 to the cap-binding complex. Forms a complex with tyf and Atx2 which functions in adult circadian pacemaker neurons to sustain circadian rhythms likely by switching between activator and repressor modes of post-transcriptional regulation via interaction with Lsm12a or me31B, respectively. The activator complex (Atx2-tyf activator complex) activates the TYF-dependent translation of per to maintain 24 hour periodicity in circadian locomotor behaviors, whereas the repressor complex (Atx2-Not1 repressor complex) promotes Not1-dependent post-transcriptional gene silencing and supports high-amplitude circadian rhythms in a per-independent manner. In 0-1 hour embryos, forms a ribonucleoprotein complex (RNP) with me31B, eIF4E1, tral and cup which binds to various mRNAs including maternal mRNAs, and downregulates their expression during the maternal-to-zygotic transition.

Gene References into Functions

PABP association with mRNA rapidly diminished with miRNA-induced silencing complex recruitment and before detectable deadenylation. PMID: 22635249
Single-molecule nanopore force spectroscopy was used to directly measure interactions between poly(A) and poly(A) binding protein. PMID: 22455926
PABP generally modulates poly(A) length and translation efficiency, neither PABP function nor GW182-PABP interaction is a prerequisite for these two silencing pathways. PMID: 22117217
Pabp binds to A-rich sequences (ARS) in the osk 3'UTR and these turned out to be required in vivo for osk function during early oogenesis. PMID: 21782810
These findings reveal that despite species-specific differences in the relative strength of the PABPC1-binding sites, the interaction between GW182 proteins and PABPC1 is critical for miRNA-mediated silencing in animal cells. PMID: 21063388
Cytoplasmic poly(A)-binding protein 1 (PABPC1) has a conserved role in nonsense-mediated mRNA decay. PMID: 17318186
Drosophila Larp associates with poly(A)-binding protein and is required for male fertility and syncytial embryo development PMID: 19631203
model proposed in which the GW182 silencing domain promotes translational repression, at least in part, by interfering with mRNA circularization and also recruits the deadenylase complex through the interaction with PABPC1 PMID: 19797087

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Protein Families

Polyadenylate-binding protein type-1 family

Database Links

KEGG: dme:Dmel_CG5119

STRING: 7227.FBpp0085915

UniGene: Dm.7069

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pAbp Antibody

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