PHYA Antibody

Code CSB-PA320415XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PHYA Polyclonal antibody
Uniprot No.
Target Names
PHYA
Alternative Names
PHYA antibody; FHY2 antibody; FRE1 antibody; HY8 antibody; At1g09570 antibody; F14J9.23 antibody; Phytochrome A antibody; Protein ELONGATED HYPOCOTYL 8 antibody; Protein FAR RED ELONGATED 1 antibody; Protein FAR RED ELONGATED HYPOCOTYL 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PHYA protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenetic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses. Pfr controls the expression of a number of nuclear genes including those encoding the small subunit of ribulose-bisphosphate carboxylase, chlorophyll A/B binding protein, protochlorophyllide reductase, rRNA, etc. It also controls the expression of its own gene(s) in a negative feedback fashion. Involved in the flowering time regulation. Can phosphorylate FHY1 and, possibly, FHL, in red light conditions; this inactivates their co-shuttling to the nucleus. Regulates phototropic responses both in the nucleus (e.g. hypocotyl elongation and cotyledon opening under high-irradiance conditions and seed germination under very-low-fluence conditions) and in the cytoplasm (e.g. negative gravitropism in blue light and red-enhanced phototropism).
Gene References into Functions
  1. phyA modulates the abundance and activity of key regulatory transcription factors in a tissue-autonomous fashion. PMID: 27027866
  2. these results suggest that lysine 206 is the main lysine for rapid ubiquitination and protein degradation of Arabidopsis phytochrome A. PMID: 26314334
  3. phytochrome-mediated degradation of PIF1 prevents over-activation of photomorphogenesis PMID: 25009301
  4. PHYA, but not PHYB, is essential for fine-tuning the chlorophyll biosynthetic pathway in response to partial shading. PMID: 24604733
  5. nuclear FHY1 can either act independently of phyA or act in association with phyA to activate the expression of distinct target genes PMID: 25071219
  6. phytochrome A function in Arabidopsis seed germination PMID: 23292879
  7. Distinct structural modules, each of which confers different properties to phyA, are assembled on the phyA molecule. PMID: 22843485
  8. We present here a hypothesis to explain how the identified amino acid substitution may lead to structural changes manifested as altered signaling and phenotype displayed by the phyA-5 mutant (which shows reduced binding affinity to FHY1/FHL). PMID: 22516823
  9. phyA controlles cry2 stability PMID: 22739826
  10. The truncated PHYA406 proteins inactivate COP1 in the nuclei in a light-independent fashion. PMID: 22498774
  11. Nuclear phyA accelerates phototropism. In the fhy1 fhl mutant, in which phyA remains in the cytosol, phototropic bending is slower than in the wild type. PMID: 22374392
  12. PHYA amino-terminal extension domain plays a role in regulating the nuclear import of PHYA. PMID: 21969386
  13. hypomethylation at a specific CG site in exon 1 was consistently associated with the release of phyA' epiallele silencing. PMID: 22466452
  14. A role for mesophyll-specific phyA in blue-light-dependent regulation of anthocyanin levels and novel roles for individual phy isoforms in the regulation of anthocyanin accumulation under red illumination, is reported. PMID: 21455024
  15. Study unravels the mechanism underlying this shift of the phyA action peak from red to far-red light and show that it relies on specific molecular interactions rather than on intrinsic changes to phyA's spectral properties. PMID: 21884939
  16. The dracula 1 (dra1) mutant, that showed no avoidance of shade for the PHYB::LUC response, was the result of a mutation in the PHYA gene. PMID: 21398429
  17. Reversible activation and repression of phyA expression by darkness and light, respectively, is accompanied by reversible changes in H3 and H4 modifications of the phyA locus. PMID: 21317377
  18. The phyA N-terminal fragment is imported into the nucleus in a light-dependent fashion. Deletion of the C-terminal domain of phyA compromises light-induced degradation of phyA in a compartmentalization-independent fashion. PMID: 21169346
  19. phyA without a nuclear localization or export signal sequence is localized exclusively in the cytoplasm in darkness. Rapid nuclear entry was observed after exposure to both red and far-red light. PMID: 20739301
  20. phyA regulates wound- and jasmonic acid-mediated JAZ1 degradation. PMID: 20435902
  21. Data show that phyA degradation is significantly slower in the cytoplasm than in the nucleus. PMID: 20473552
  22. Results suggest that the autophosphorylation of phyA plays an important role in the regulation of plant phytochrome signaling through the control of phyA protein stability. PMID: 20203237
  23. Genetic variations in the PhyA locus of A. thaliana inbred strains and the effects on seedling growth under infrared light are reported. PMID: 15908601
  24. The potential of manipulating light signal-transduction pathways to minimize the problems of lodging in basmati/aromatic rice and to enhance grain productivity is reported. PMID: 16136335
  25. data indicate that phyA, the 26S proteasome, and the Constitutive Photomorphogenic/De-Etiolated/Fusca proteins are all involved in the light regulation of FHY1 protein abundance during Arabidopsis (Arabidopsis thaliana) seedling development PMID: 16244150
  26. PHYC was the most strongly associated locus across 163 strains, suggesting that PHYC alleles are under diversifying selection in A. thaliana PMID: 16732287
  27. This study showed that phyA has a quantitatively dominant role in Rc-induced expression of early response genes in A. thaliana seedlings. PMID: 17076805
  28. The serine-rich region is involved in negative regulation of phyA signaling. PMID: 17160561
  29. Results raise the possibility that phyA may contribute significantly to the regulation of growth and development in daylight-grown plants. PMID: 17346261
  30. Data showed a transcriptionally suppressed epi-allele of phytochrome A gene (PHYA) methylated only in symmetric CG sites resident in exonic regions, resulting in elongated hypocotyls in seedlings grown under continuous far-red light. PMID: 17931351
  31. required for the up-regulation of PEX11b in the light PMID: 18203870
  32. The expression levels of polyamine biosynthesis-related genes were wtranscribed less in the wild type than in phyA seedlings under both light conditions. PMID: 18375607
  33. Data show that phyA plays a dominant role in regulating the degradation of PIF1 following initial light exposure and PIF1 interacts with phyA through a novel active phyA binding motif. PMID: 18539749
  34. FHY1 and FHL work as adaptor proteins facilitating nuclear transport of phyA. PMID: 18670649
  35. Phytochrome A mediates the localization of phot1 in Arabidopsis. PMID: 18952772
  36. The G-protein may be involved in the phyA signaling pathway to regulate far-red irradiation-preconditioned cell death of Arabidopsis hypocotyls. PMID: 19160542
  37. Phytochrome A is required for the phosphorylation of FHY1. PMID: 19208901
  38. A missense mutation in the PHYA histidine kinase-related domain alters the spectral sensitivity and the persistence of far-red light-induced high-irradiance responses. PMID: 19403732

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Subcellular Location
Cytoplasm. Nucleus, nucleoplasm. Nucleus speckle.
Protein Families
Phytochrome family
Database Links

KEGG: ath:AT1G09570

STRING: 3702.AT1G09570.1

UniGene: At.22828

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