PIF1 Antibody

Code CSB-PA812763XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PIF1 Polyclonal antibody
Uniprot No.
Target Names
PIF1
Alternative Names
PIF1 antibody; BHLH15 antibody; EN101 antibody; PIL5 antibody; At2g20180 antibody; T2G17.2 antibody; Transcription factor PIF1 antibody; Basic helix-loop-helix protein 15 antibody; AtbHLH15 antibody; bHLH 15 antibody; Protein PHY-INTERACTING FACTOR 1 antibody; Protein PHYTOCHROME INTERACTING FACTOR 3-LIKE 5 antibody; Transcription factor EN 101 antibody; bHLH transcription factor bHLH015 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PIF1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription activator. Regulates negatively chlorophyll biosynthesis and seed germination in the dark, and lightinduced degradation of PIF1 relieves this negative regulation to promote photomorphogenesis. Binds to the G-box motif (5'-CACGTG-3') found in many light-regulated promoters. Promotes the expression of SOM, and thus modulates responses to abscisic acid (ABA) and gibberellic acid (GA).
Gene References into Functions
  1. PIL5 is involved in floral transition interacting with flowering integrators and gibberellic acid. PMID: 29588173
  2. The genetic, physiological, and biochemical evidence, when taken together, leads us to propose that PIF1 and CTG10 coexist, and even accumulate, in the nucleus in darkness, but that, following illumination, CTG10 assists in reducing PIF1 amounts, thus promoting the completion of seed germination and subsequent seedling development. PMID: 29632208
  3. results reveal a new mechanism for NO signals in modulating PHYB-mediated seed germination by repressing PIF1 expression at the transcriptional level as well as preventing PIF1 activity by stabilizing HFR1 protein. PMID: 29248678
  4. COP1 promotes the degradation of HFR1 under shade, thus increasing the ability of PIFs to control gene expression, increase auxin levels and promote stem growth. PMID: 27105120
  5. the transcriptional cascade consisting of PIF1/PIF3, HY5, and BBX23 controls photomorphogenesis. PMID: 28687557
  6. results indicate that RPGE1 acts downstream of PIF1 in the endodermis to repress photosynthetic genes and regulate plastid development. PMID: 27591813
  7. Data we show in vivo PIF1 targeting to specific binding sites is determined by its interaction with PTFs and their binding to GCEs. PMID: 27303023
  8. Results indicate that LEUNIG_HOMOLOG (LUH) functions with PHYTOCHROME-INTERACTING FACTOR1 (PIF1) as a transcriptional coregulator to inhibit seed germination. PMID: 26276832
  9. The results suggest that AtHB1 acts downstream of PIF1 to promote hypocotyl elongation, especially in response to short-day photoperiods. PMID: 25865500
  10. In vivo results support a regulatory mechanism for PIFs in which HMR is a transcriptional coactivator binding directly to PIFs and the 9aaTAD of HMR couples the degradation of PIF1 and PIF3 with the transactivation of PIF target genes. PMID: 25944101
  11. DET1 maximizes PIF1's action by both removing PIF1's transcriptional repressor HFR1 and protecting PIF1 from proteasome-mediated degradation. PMID: 25775589
  12. PIF1, PIF3, PIF4, and PIF5 act together to promote and optimize growth under photoperiodic conditions. PMID: 24420574
  13. EFS is necessary for high-level expression of PIF1 mRNA in imbibed seeds. PMID: 24635727
  14. VQ29 is a negative transcriptional regulator of light-mediated inhibition of hypocotyl elongation that likely promotes the transcriptional activity of PIF1 during early seedling development. PMID: 24569844
  15. HFR1 prevents PIF1 from binding to its target genes and antagonistically regulates PIF1-mediated gene expression. HFR1 and PIF1 are the major transcription regulators responsible for light-directed Transcriptome changes in seed germination. PMID: 24179122
  16. Data indicate that the PIF1/PIF3-HY5/HYH transcriptional modules mediate crosstalk between light and ROS signaling and a mechanism by which plants adapt to the light environments. PMID: 23645630
  17. The ability of Glc to induce IAA biosynthesis was upregulated in the pif1 pif3 pif4 pif5 quadruple mutant line compared with the wild type. PMID: 23209113
  18. Transposase-derived proteins FHY3/FAR1 interact with PHYTOCHROME-INTERACTING FACTOR1 to regulate chlorophyll biosynthesis by modulating HEMB1 during deetiolation in Arabidopsis. PMID: 22634759
  19. ABI3 and PIL5 collaboratively activate the expression of SOM mRNA by directly binding to and interacting with each other at the SOM promoter. PMID: 21467583
  20. Data suggest that CK2-mediated phosphorylation enhances the light-induced degradation of PIF1 to promote photomorphogenesis. PMID: 21330376
  21. PIF1 and other PIFs have roles in transducing light signals to regulate PSY gene expression and carotenoid accumulation during daily cycles of light and dark in mature plants PMID: 20534526
  22. Data show that AtGA3ox1 is directly regulated by DAG1, and suggest that DAG1 is not a direct regulatory target of PIL5. PMID: 19874540
  23. negatively regulates chlorophyll biosynthesis PMID: 15448264
  24. PIF1 is degraded in red (R) and far-red (FR) light through the ubiquitin (ub)-26S proteasome pathway to optimize photomorphogenic development in Arabidopsis.[PIF1] PMID: 16359394
  25. PIL5 binds directly to GAI and RGA promoters but not to Giberellic and Abscisic metabolic gene promoters in arabidopsis. PMID: 17449805
  26. Data shows that PIL5 activates the expression of SOM by binding directly to its promoter, suggesting that PIL5 regulates ABA and GA metabolic genes partly through SOM. PMID: 18487351
  27. Data suggest that removal of PIF1 by light-induced proteolytic degradation might be sufficient to promote photomorphogenesis. PMID: 18539749
  28. These data strongly suggest that PIF1 directly and indirectly regulates key genes involved in chlorophyll biosynthesis to optimize the greening process in Arabidopsis. PMID: 18591656
  29. PIL5 inhibits seed germination not just through GA and ABA, but also by coordinating hormone signals and modulating cell wall properties in imbibed seeds. PMID: 19244139

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed constitutively in roots, leaves, stems, and flowers.
Database Links

KEGG: ath:AT2G20180

STRING: 3702.AT2G20180.2

UniGene: At.43003

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