PIF4 Antibody

Code CSB-PA848762XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PIF4 Polyclonal antibody
Uniprot No.
Target Names
PIF4
Alternative Names
PIF4 antibody; BHLH9 antibody; EN102 antibody; SRL2 antibody; At2g43010 antibody; MFL8.13 antibody; Transcription factor PIF4 antibody; Basic helix-loop-helix protein 9 antibody; AtbHLH9 antibody; bHLH 9 antibody; Phytochrome-interacting factor 4 antibody; Short under red-light 2 antibody; Transcription factor EN 102 antibody; bHLH transcription factor bHLH009 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PIF4 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor acting negatively in the phytochrome B signaling pathway. May regulate the expression of a subset of genes involved in cell expansion by binding to the G-box motif. Activated by CRY1 and CRY2 in response to low blue light (LBL) by direct binding at chromatin on E-box variant 5'-CA[CT]GTG-3' to stimulate specific gene expression to adapt global physiology (e.g. hypocotyl elongation in low blue light).
Gene References into Functions
  1. The interaction between TOC1 and PIF4 mediates the circadian gating of thermoresponsive growth. PMID: 27966533
  2. DCL1 interacts with PIF4.PIF4 acts as a transcription factor for some miRNA genes and is necessary for the proper accumulation of miRNAs. PMID: 29522510
  3. Study shows that PHYTOCHROME INTERACTING FACTOR 4 (PIF4)-mediated thermosensory growth and architecture adaptations are directly linked to suppression of immunity at elevated temperature. PIF4 positively regulates growth and development and negatively regulates immunity. PMID: 28041792
  4. Results show that the phytochrome-interacting factor 4 (PIF4) is required for cytokinin (CK) upregulation of the transcription of CKRC2/YUC8 and that the transcription of PIF4 itself is induced by CK via the AHKs-ARR1/12 signaling pathway. PMID: 27827441
  5. Analysis of transcriptomes indicated that increased expression of the transcription factor PHYTOCHROME-INTERACTING FACTOR (PIF4) may contribute to hybrid vigor by targeting the auxin biosynthesis gene YUCCA8 and the auxin signaling gene IAA29. PMID: 28396418
  6. BAF60 binds nucleosome-free regions of multiple G box-containing genes, opposing in cis the promoting effect of the photomorphogenic and thermomorphogenic regulator Phytochrome Interacting Factor 4 (PIF4) on hypocotyl elongation. Furthermore, BAF60 expression level is regulated in response to light and daily rhythms. PMID: 28619072
  7. COG1 binds to the promoter regions of PIF4 and PIF5, and PIF4 and PIF5 bind to the promoter regions of key Brassinosteroid biosynthetic genes, such as DWF4 and BR6ox2, to directly promote their expression. PMID: 28438793
  8. These findings reveal a mechanistic framework in which DET1/COP1 and HY5 regulatory modules act independently to regulate growth through the environmental signal integrator PIF4. PMID: 28076780
  9. ethylene-insensitive 3 (EIN3) is involved into the direct regulation of YUC9 transcription in this process. Furthermore, we demonstrated that PHYTOCHROME INTERACTING FACTOR4 (PIF4) functions as a transcriptional activator for YUC5/8/9. PMID: 27716807
  10. this review discusses PIF4 as a key integrator of multiple signaling pathways, which optimizes growth in the environment PMID: 27432188
  11. LEC1 acts as a coactivator of PIFs in transcriptional regulation during postembryonic growth. PMID: 26566918
  12. For growth under a canopy, where blue light is diminished, CRY1 and CRY2 perceive this change and respond by directly contacting two bHLH transcription factors, PIF4 and PIF5. PMID: 26724867
  13. CRY1 represses auxin biosynthesis in response to elevated temperature through PIF4. PMID: 26699514
  14. PIF4 and PIF5 negatively regulate red light-induced anthocyanin accumulation through transcriptional repression of the anthocyanin biosynthetic genes in Arabidopsis. PMID: 26259175
  15. Phytochrome-interacting transcription factors PIF4 and PIF5 induce leaf senescence in Arabidopsis. PMID: 25119965
  16. The expression level of PIF3, 4, and 5 was significantly up-regulated during both age-triggered and dark-induced leaf senescence. PMID: 25296857
  17. At least two downstream modules participate in diurnal rhythmic hypocotyl growth: PIF4 and/or PIF5 modulation of auxin-related pathways and PIF-independent regulation of the gibberellic acid (GA) pathway. PMID: 21430186
  18. The PIF4 and PIF5 transcription factors promote flowering by at least two means: inducing FT expression in warm night and acting outside of FT by an unknown mechanism in warm days. PMID: 24574484
  19. the light-regulated PIF4 (phytochrome-interacting factor 4) factor is a phosphorylation target of the BR signaling kinase BRASSINOSTEROID-INSENSITIVE 2 (BIN2), which marks this transcriptional regulator for proteasome degradation PMID: 25085420
  20. circadian clock and PIF4/PIF5 mediated external coincidence mechanism in transcription of ST2A PMID: 24317064
  21. Data indicate that PIF4 and PIF5 negatively regulate auxin signaling. that PIF4 and PIF5 negatively modulate auxin-mediated phototropism through directly activating IAA19 and IAA29, which physically interact with auxin factor7 (ARF7). PMID: 23757399
  22. PIF4 and/or PIF5 act as modulators of auxin signaling implicated in the rhythmic elongation of hypocotyls. PMID: 23037003
  23. Circadian clock affects the expression of PIF4 in response to high temperature. PMID: 23037004
  24. regulates elongation growth by controlling directly the expression of genes that code for auxin biosynthesis and auxin signaling components PMID: 22536829
  25. PhyB-mediated, post-translational regulation allows PIF3 accumulation to peak just before dawn, at which time it accelerates hypocotyl growth, together with PIF4 and PIF5, by directly regulating the induction of growth-related genes. PMID: 22409654
  26. BZR1 and PIF4 interact with each other in vitro and in vivo, bind to nearly 2,000 common target genes, and synergistically regulate many of these target genes PMID: 22820378
  27. our results illuminate a molecular framework by which the PIF4 transcriptional regulator integrates its action into the auxin pathway through activating the expression of specific auxin biosynthetic gene (YUCCA8). PMID: 22479194
  28. demonstration of a direct mechanism by which increasing temperature causes the bHLH transcription factor PHYTOCHROME INTERACTING FACTOR4 (PIF4) to activate FLOWERING LOCUS T (FT) PMID: 22437497
  29. These results demonstrate direct molecular links among PIF4, auxin, and elongation growth at high temperature. PMID: 22123947
  30. an external coincidence model involving the clock-controlled PIF4/PIF5-ATHB2 pathway is crucial for the diurnal and photoperiodic control of plant growth in A. thaliana. PMID: 21666227
  31. PIF4 and PIF5 responsible not only for red light signaling through the phytochromes but also for blue light signaling in the photomorphogenic control of hypocotyl elongation PMID: 21150090
  32. A non-synonymous coding SNP at PIF4 is associated significantly with variation in average early internode length and marginally associated with total inflorescence length. PMID: 20456226
  33. microarray analysis shows that PIF4 and PIF5 are part of an inhibitory mechanism that represses the expression of some light-responsive genes in the dark, and that they are also needed for full expression of several growth-related genes in the light. PMID: 19619162
  34. Overexpression of SHORT HYPOCOTYL UNDER BLUE1 enhanced the expression of PHYTOCHROME-INTERACTING FACTOR4 (PIF4) under red light. PMID: 16500988
  35. Data provide evidence that the mechanism by which PIF4 operate on the phyB signaling pathway under prolonged red light is through maintaining low phyB protein levels, in an additive or synergistic manner. PMID: 18252845
  36. PIF4 Acts in a phytochrome-Dependent Manner to Mediate Changes in Stomatal Index. PMID: 19185498
  37. Results suggest that PIF4 is an important component of plant high temperature signaling and integrates multiple environmental cues during plant development. PMID: 19249207

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Subcellular Location
Nucleus.
Protein Families
BHLH protein family
Database Links

KEGG: ath:AT2G43010

STRING: 3702.AT2G43010.1

UniGene: At.19015

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