piwi Antibody

1. PAF1 knockdown enhances PIWI silencing of reporters when Piwi-interacting RNAs (piRNAs)-target the transcript region proximal to the promoter. Loss of PAF1 suppresses endogenous transposable element (TE) transcript maturation, whereas a subset of gene transcripts and long-non-coding RNAs adjacent to TE insertions are affected by PAF1 knockdown in a similar fashion to piRNA-targeted reporters. PMID: 28844648
2. These data identify a role for Piwi in the regulation of somatic stem cell function. PMID: 28903034
3. Data show that Cbp80 knockdown not only led to the upregulation of transposons, but also to delocalization of Piwi, Aub and Ago3, key factors in the piRNA biosynthesis pathway. PMID: 28746365
4. Report the mapping of multiple Piwi-interacting chromosomal domains in somatic cells of ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes. PMID: 28472469
5. The identity of piRNA clusters is epigenetically acquired in a Piwi-dependent manner during embryonic development. PMID: 28457744
6. Piwi-piRNA complexes regulate the interaction of chromatin components with target loci to maintain silencing of transposable elements through the modulation of chromatin accessibility. PMID: 27425411
7. Analysis of piRNA clusters containing remnants of I-elements shows increased expression of the piRNA (PIWI-interacting RNA )precursors and enrichment by the Heterochromatin Protein 1 homolog, Rhino, in weak R strains, which is in accordance with stronger piRNA expression by these regions PMID: 28448516
8. Piwi represses c-Fos in the somatic niche to regulate germ stem cell maintenance and differentiation and in the somatic follicle cells to affect morphogenesis. PMID: 27622269
9. We find there are large differences in evolutionary rates and gene turnover between pathways, and that paralogs of Ago2, Ago3, and Piwi/Aub show contrasting rates of evolution after duplication. PMID: 26868596
10. dFMRP cooperates with Piwi in maintaining genome integrity by silencing heterochromatic genes and suppressing transposon expression. PMID: 26842990
11. We hypothesize that Piwi negatively regulates PRC2 binding by sequestering PRC2 in the nucleoplasm, thus reducing PRC2 binding to many targets and influencing transcription during oogenesis. PMID: 26780607
12. Piwi nuclear localization in somatic cells is required for differentiation. Piwi regulates the somatic expression of Fasciclin 3 in gonadal development. PMID: 26119740
13. Functions of piRNAs and the Piwi Protein in Drosophila PMID: 26087619
14. These results implicate Piwi in RNA polymerase activity modulation and stress response in the nucleolus. We discuss possible noncanonical Piwi functions along with its canonical role in transposon silencing by piRNAs. PMID: 25916123
15. This reporter platform will facilitate future dissections of the PIWI-targeting mechanism. PMID: 25336588
16. The results lead us to propose that the interaction between Su(var)3-7 and piwi or aubergine controls important developmental processes independently of transposon silencing. PMID: 24820312
17. Piwi is required in multiple cell types to control gerline stem cell lineage development in the Drosophila ovary. PMID: 24658126
18. The results demonstrate that a significant increase in the H3K4me2 level upon nuclear Piwi loss is not accompanied by the alterations in H3K9me3 and HP1a levels for several germline-expressed transposons, suggesting that in this case Piwi prevents transcription by a mechanism distinct from H3K9 methylation. PMID: 24782529
19. HP1a profiles in piwi mutant animals confirm that Piwi depletion leads to decreased HP1a levels in pericentric heterochromatin, particularly in transposable elements . PMID: 24068954
20. Drosophila piwi mutants exhibit germline stem cell tumors that are sustained by elevated Dpp signaling.The direct requirement for Piwi for normal germline formation is largely restricted to larval stages. PMID: 23891114
21. REVIEW: new findings reveal the Piwi-piRNA pathway as a more general mechanism of gene regulation PMID: 23465540
22. Piwi plays multiple roles in the piRNA pathway, in part enforcing transposon repression through effects on local chromatin states and transcription but also participating in germ cell piRNA biogenesis PMID: 23392609
23. Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID: 23392610
24. in transgenic flies with the DDX catalytic triad of PIWI mutated, neither primary nor secondary piRNA biogenesis is detectably affected, transposons remain repressed, and fertility is normal PMID: 23297219
25. Drosophila Piwi functions downstream of piRNA production mediating a chromatin-based transposon silencing mechanism in female germ line. PMID: 22160707
26. Piwi function in germ-line stem cell (GSC) self-renewal is independent of transposon repression and is normally realized in the cytoplasm of GSC niche cells PMID: 22065765
27. Piwi functions in Hsp90-mediated suppression of phenotypic variation PMID: 21186352
28. results reveal a novel epigenetic mechanism involving Corto and Piwi that defines the fate and signaling function of niche cells in maintaining germline stem cells PMID: 20647505
29. The impairing aubergine and piwi mutations also increase the level of germ-line mRNA from CP, a transgene that encodes the P-element transposase. PMID: 20943007
30. Studies show that Drosophila melanogaster has three PIWI proteins: PIWI, AUBERGINE (AUB), and ARGONAUTE3 (AGO3) and it has been shown that Drosophila PIWI is mostly a nuclear protein [10] and is involved in the asymmetric division of germline stem cells. PMID: 19774126
31. Mutations in piwi block posttranscriptional gene silencing of transgenic Adh. PMID: 11864605
32. mutations in piwi, aubergine, or spindle-E which encode RNAi components result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2 PMID: 14752161
33. Piwi maintains germline stem cells by repressing Bam expression, thus preventing Bam from downregulating Pum/Nos function in repressing the translation of differentiation genes and germline Piwi function in promoting germ cell division. PMID: 15668175
34. Piwi maintains germline stem cells by silencing bam. PMID: 15668176
35. PIWI protein is involved in silencing of LTR retrotransposons in testes. PMID: 15817569
36. A genome-wide analysis of mRNAs regulated by piwi was performed. PMID: 16581772
37. These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with repeat-associated small interfering RNAs originating from repetitive targets. PMID: 16882972
38. Study examined piwi-interacting RNAs (piRNAs) associated with each Drosophila Piwi protein and found that Piwi and Aubergine bind RNAs that are predominantly antisense to transposons, whereas Ago3 complexes contain predominantly sense piRNAs. PMID: 17346786
39. Pimet mediates piwi-interacting RNAs 2'-O-methylation in Drosophila. PMID: 17606638
40. Piwi promotes euchromatic histone modifications and piRNA transcription in subtelomeric heterochromatin (also known as telomere-associated sequence, or TAS), on the right arm of chromosome 3 PMID: 17952056
41. somatic silencing of two retrotransposons depends on Piwi in the follicle cells surrounding the oocyte. PMID: 18253480
42. The Drosophila homologue of protein methyltransferase 5 (dPRMT5, csul/dart5)methylates arginine in Drosophila Piwi, Ago3 and Aub proteins in vivo. Arginine methylation of Piwi proteins is required for Ago3 and Aub stability. PMID: 19377467
43. in gonadal somatic cells, tj gives rise simultaneously to two different molecules: the TJ protein, which activates Piwi expression, and piRNAs, which define the Piwi targets for silencing PMID: 19812547

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KEGG: dme:Dmel_CG6122

STRING: 7227.FBpp0079755