Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PKL Polyclonal antibody

Uniprot No.

Q9S775

Target Names

PKL

Alternative Names

PKL antibody; CHR6 antibody; GYM antibody; At2g25170 antibody; F13D4.130 antibody; CHD3-type chromatin-remodeling factor PICKLE antibody; EC 3.6.4.- antibody; Protein CHROMATIN REMODELING 6 antibody; AtCHR6 antibody; Protein GYMNOS antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PKL protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Chromatin remodeling factor that represses the expression of embryonic trait genes (such as NFYB9/LEC1) upon and after seed germination and thus enables the developmental switch to post-germinative growth. Silences some MADS-box proteins such as PHE1 and PHE2. Plays a role during carpel differentiation. Regulates late processes in cytokinin signaling.

Gene References into Functions

The antagonistic functions of FIS1 and PKR2 in modulating endosperm development are similar to those of PICKLE (PKL) and CURLY LEAF (CLF), which antagonistically regulate root meristem activity. PMID: 28155248
Pickle role in the vegetative growth and gibberellin signaling PMID: 28057895
Plants lacking the CHD3 remodeler, PICKLE, exhibit various reproductive defects. These phenotypes result from loss of PICKLE in the maternal sporophyte. PMID: 27075727
data suggest that transcriptional regulation of LFY at the chromatin level by PKL may at least partially account for the late-flowering phenotype of pkl mutants PMID: 27056257
EPP1 acts additively with SPA1 to repress seedling de-etiolation. PMID: 23733056
PKL physically interacts with HY5 to directly regulate hypocotyl cell elongation by repressing trimethylation of histone H3 Lys 27 (H3K27me3) on the regulatory regions of several cell elongation- related genes in response to changing light conditions. PMID: 23314848
These studies demonstrate that subfamily II CHD proteins in plants, such as PICKLE, retain ATP-dependent chromatin remodeling activity PMID: 23128324
CKH1/EER4/AtTAF12b and CKH2/PKL may act together on cytokinin-regulated genes. PMID: 21357580
PKL plays a role in chromatin homeostasis. PMID: 22452853
Loss of PKL function impairs root growth in Arabidopsis. PMID: 21441433
PKL is required continuously after germination to repress expression of PHERES1, a type I MADS box gene that is normally expressed during early embryogenesis in wild-type plants. PMID: 16359393
PKL-dependent repression of embryonic gene expression extends to late-embryogenesis genes and is associated with changes in chromatin. PMID: 17892443
PKL promotes hisone h3 methylation in both germinating seedlings and in adult plants but do not identify a connection between PKL-dependent expression and acetylation levels. PMID: 18539592
PKL and PKR2 are required for the expression of many genes that are repressed by PcG proteins. PMID: 19680533

Hide All

Subcellular Location

Nucleus.

Protein Families

SNF2/RAD54 helicase family

Tissue Specificity

Mostly expressed in tissue undergoing significant differentiation (meristems and primordia) such as young seedlings, influorescent tissue and young siliques, but not in endosperm and seed coat (at protein level). Levels decrease as organs age. Also presen

Database Links

KEGG: ath:AT2G25170

STRING: 3702.AT2G25170.1

UniGene: At.11745

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA879675XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

PKL Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background