PSKR1 Antibody

Code CSB-PA978516XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PSKR1 Polyclonal antibody
Uniprot No.
Target Names
PSKR1
Alternative Names
PSKR1 antibody; PSKR antibody; At2g02220 antibody; F5O4.1 antibody; Phytosulfokine receptor 1 antibody; AtPSKR1 antibody; EC 2.7.11.1 antibody; EC 4.6.1.2 antibody; Guanylate cyclase antibody; Phytosulfokine LRR receptor kinase 1 antibody; Protein serine-threonine kinase antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PSKR1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Phytosulfokine receptor with both a serine/threonine-protein kinase activity and a guanylate cyclase activity. Regulates, in response to phytosulfokine binding, a signaling cascade involved in plant cell differentiation, organogenesis, somatic embryogenesis, cellular proliferation and plant growth. Involved in plant immunity, with antagonistic effects on bacterial and fungal resistances. Not involved in PSY perception. CNGC17 and AHAs form a functional cation-translocating unit that is activated by PSKR1/BAK1 and possibly other BAK1/RLK complexes.
Gene References into Functions
  1. Peptide signaling of growth by PSKR1 is regulated by differential phosphorylation of the juxtamembrane and C-terminal domains of the intracellular receptor part and suggest that interaction of PSKR1 with calmodulin serves a function other than the regulation of kinase activity. PMID: 28338789
  2. Phytosulfokine signalling through PSKR1 stimulates the Hyaloperonospora arabidopsidis infection cycle. PMID: 26290138
  3. Size exclusion and analytical ultracentrifugation analysis of the PSKR1cd suggest that it is reversibly dimeric in solution, which was further confirmed by biflourescence complementation. Taken together, these data suggest that in this novel type of receptor domain architecture, specific phosphorylation and dimerization are possibly essential mechanisms for ligand-mediated catalysis and signaling PMID: 27487840
  4. Conserved phosphorylation sites in the activation loop of the Arabidopsis phytosulfokine receptor PSKR1 differentially affect kinase and receptor activity. PMID: 26472115
  5. Calcium is the switch in the moonlighting dual function of PSKR1 PMID: 25245092
  6. data reveal the structural basis for PSKR recognition of PSK and allosteric activation of PSKR by PSK, opening up new avenues for the design of PSKR-specific small molecules PMID: 26308901
  7. PSK signaling requires Ca(2+) /CaM binding and kinase activity of PSKR1 in planta. PMID: 24495073
  8. PSK signaling mediated by PSKR1 attenuates pattern-triggered immunity but stimulates growth. PMID: 22353039
  9. the tyrosine sulfated peptide receptors PSKR1 and PSY1R are not only involved in growth and development but also in plant defense. PMID: 23470723
  10. Overexpression of PSKR1 promotes hypocotyl elongation. PMID: 23083315
  11. PSK-alpha signaling of hypocotyl elongation and protoplast expansion occurs through PSKR1 and likely involves K(+) uptake, but does not require extracellular acidification by the plasma membrane H(+)-ATPase PMID: 21698171
  12. PSKR1 contains dual GC and kinase catalytic activities PMID: 21504901
  13. PSK-alpha signaling through AtPSKR1 affects root elongation primarily via control of mature cell size. PMID: 19076296
  14. Fungal infection induces expression of PSK2, PSK5 and PSKR1 and alters photosynthetic performance in PSK receptor mutants. Wounding induces expression of PSK3, PSK5 and PSKR1. PMID: 20403122
  15. it is reported that loss-of-function and gain-of-function mutations of the phytosulfokine receptor gene (AtPSKR1) alter cellular longevity and potential for growth without interfering with basic morphogenesis of plants PMID: 16829587

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
Weakly expressed in roots, leaves, stems and flowers. Expressed in the primary and lateral roots, including root primordia and root tips, but not in the hypocotyl.
Database Links

KEGG: ath:AT2G02220

STRING: 3702.AT2G02220.1

UniGene: At.28244

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