Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans daf-18 Polyclonal antibody

Uniprot No.

G5EE01

Target Names

daf-18

Alternative Names

daf-18 antibody; T07A9.6 antibody; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase daf-18 antibody; EC 3.1.3.16 antibody; EC 3.1.3.48 antibody; EC 3.1.3.67 antibody; Abnormal dauer formation protein 18 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans daf-18 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate. By dephosphorylating PtdIns(3,4,5)P3 antagonizes PtdIns(3,4,5)P3 production by age-1/PI3K and thus, negatively regulates daf-2-mediated processes including dauer formation, longevity, fat metabolism, chemotaxis towards salt, thermotolerance and axon guidance. Similarly, promotes apoptosis during embryonic development by suppressing the recruitment of the prosurvival kinases akt-1/2 to the plasma membrane. In addition, regulates Z2/Z3 germline precursor cell cycle by maintaining them arrested at the G2 stage and by controlling their growth during L1 diapause. After sperm depletion in larvae and adult hermaphrodites, promotes germline stem cell quiescence and oocyte accumulation. By dephosphorylating ephrin-like receptor vab-1 on tyrosine residues, negatively regulates oocyte maturation downstream of vab-1 and upstream of mpk-1, independently of daf-2. Plays a role in postembryonic muscle arm extensions. Required for neurite outgrowth during AIY interneuron embryonic development. Mainly independently of daf-2, negatively regulates vulva induction probably by inhibiting mpk-1 phosphorylation. Both lipid and protein phosphatase activities are required for the regulation of vulva induction.

Gene References into Functions

differential regulation of germline stem cell proliferation rates in Caenorhabditis elegans adults is accomplished through localized neutralization of insulin/IGF-1 signalling, requiring DAF-18/PTEN. PMID: 26552888
Doxycyclin ameliorates a starvation-induced germline tumor in C. elegans with a daf-18/PTEN mutant background. PMID: 24746511
Mutations in the PTEN (DAF-18) tumor suppressor gene may result in the simultaneous hyperactivation of two oncogenic signaling pathways. PMID: 22916028
DAF-18 modulates the PI3K signaling pathway to activate DAF-16/FOXO and promote developmental neurite outgrowth. PMID: 22069193
complete dauer formation requires daf-18 expression in several tissues,and the remodeling of dauer tissues depends on both cell autonomous and cell nonautonomous daf-18 function(s) PMID: 16153634

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Subcellular Location

Perikaryon. Cell membrane; Peripheral membrane protein. Cell projection, axon. Cell projection, dendrite. Cytoplasm. Nucleus.

Tissue Specificity

Expressed in embryo, larvae and in adult germline (at protein level). Expressed at equal levels in the 6 vulva precursor cells (VPCs) of L2 larvae and in the descendant cells of the induced VPCs (at protein level). Expressed in the uterus (at protein leve

Database Links

KEGG: cel:CELE_T07A9.6

STRING: 6239.T07A9.6

UniGene: Cel.38708

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daf-18 Antibody

Product Details

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Target Background