Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RANGAP1 Polyclonal antibody

Uniprot No.

Q9LE82

Target Names

RANGAP1

Alternative Names

RANGAP1 antibody; At3g63130 antibody; T20O10.230 antibody; RAN GTPase-activating protein 1 antibody; AtRanGAP1 antibody; RanGAP1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RANGAP1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. Plays a role in spatial signaling during cell division.

Gene References into Functions

The data show that plant development is differentially affected by RanGAP mutant allele combinations of increasing severity and requires the GAP activity of RanGAP, while the subcellular positioning of RanGAP is dispensable. PMID: 26091693
These results show that the two RanGAPs are redundant and indispensable for female gametophyte development in Arabidopsis but dispensable for pollen development. PMID: 21282324
AtRanGAP1 has a mitotic trafficking pattern uniquely different from that of vertebrate RanGAP. PMID: 15807788
In a wip1-1/wip2-1/wip3-1 triple mutant, RanGAP1 is dislocated from the nuclear envelope in undifferentiated root-tip cells, whereas nuclear envelope targeting in differentiated root cells and targeting to the cell plate remain intact. PMID: 17600715
Data suggest that an unanticipated complexity of RanGAP nuclear envelope targeting and at least one member of each NE-associated coiled-coil and transmembrane domains protein family is required for RanGAP targeting in root tip cells. PMID: 18591351
propose that Arabidopsis RanGAP, a continuous positive protein marker of the plant division plane, has a role in spatial signaling during plant cell division PMID: 19011093
These data suggest that both HSC70-1 and the WPP-domain proteins play a role in facilitating WIT1 nuclear envelope targeting; this may be the first described in planta activity for the WPP-domain proteins. PMID: 19617588

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Subcellular Location

Cytoplasm. Nucleus envelope. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, phragmoplast. Note=Localized in patchy areas at the nuclear envelope (NE) of interphase cells. Concentrates at the preprophase band (PPB) and remains associated with the cortical division site (CDS) during mitosis and cytokinesis. During mitosis, associates with mitotic spindles at the anaphase. Associated to the microtubular phragmoplast and the surface of the daughter nuclei at the telophase.

Protein Families

RNA1 family

Database Links

KEGG: ath:AT3G63130

STRING: 3702.AT3G63130.1

UniGene: At.48804

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RANGAP1 Antibody

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Target Background