Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RDR6 Polyclonal antibody

Uniprot No.

Q9SG02

Target Names

RDR6

Alternative Names

RDR6 antibody; RDRP6 antibody; SDE1 antibody; SGS2 antibody; At3g49500 antibody; T1G12.20 antibody; T9C5.95 antibody; RNA-dependent RNA polymerase 6 antibody; AtRDRP6 antibody; EC 2.7.7.48 antibody; Protein SILENCING DEFECTIVE 1 antibody; Protein SUPPRESSOR OF GENE SILENCING 2 antibody; RNA-directed RNA polymerase 6 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RDR6 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

RNA-dependent RNA polymerase involved in post-transcriptional gene silencing (PTGS). Possesses ssRNA and ssDNA-dependent polymerase activity, but does not have priming activity. Possesses in vitro 3' nucleotidyltransferase activity in the presence of UTP as single nucleotide. Required for the production of 21 nucleotide trans-acting small interfering RNAs (ta-siRNAs) derived from TAS1, TAS2 and TAS3 endogenous transcripts. Acts in the RDR6/SGS3/DCL4/AGO7 ta-siRNA pathway involved in leaf developmental timing. Required for the production of natural siRNAs (nat-siRNAs) derived from cis-natural antisense transcripts. Required for the production of 24 nucleotide nat-siRNAs derived from the stress-related P5CDH-SRO5 antisense gene pair. Required for PTGS induced by transgene direct repeats. Plays an essential role in transitive silencing of transgenes by processing secondary siRNAs. This pathway, which requires DCL2 and DCL4, amplifies silencing by using the target RNA as substrate to generate secondary siRNAs, providing an efficient mechanism for long-distance silencing. Involved in the biogenesis of secondary siRNAs which require 22 nucleotide miRNAs associated to AGO1. Participates synergistically with AS1 and AS2 to proper plant development by repressing the miR165 and miR166 microRNAs (independently of AGO10) that may lead to mRNA degradation of genes in the class III HD-ZIP family. Required for the production of some small RNAs derived from the crucifer-infecting tobamovirus (TMV-cg). Required for sense virus-induced post-transcriptional gene silencing (S-PTGS).

Gene References into Functions

The results show that the expression of VPg alone, independent of viral infection, is sufficient to induce the degradation of SGS3 and its intimate partner, RDR6. PMID: 27795417
Mungbean yellow mosaic Indian virus AC2 interferes with the biogenesis of siRNA by interacting and blocking the RDR6 activity. PMID: 26433748
Data show that the lethality of decapping mutants is suppressed by impairing RNA-dependent RNA polymerase 6 (RDR6) activity. PMID: 25694514
The Arabidopsis miR472-RDR6 silencing pathway modulates PAMP- and effector-triggered immunity through the post-transcriptional control of disease resistance genes. PMID: 24453975
RDR6-RdDM functions to recognize active Polymerase II-derived Transposable element (TE) mRNA transcripts to both trigger and correctively reestablish TE methylation and epigenetic silencing. PMID: 23542151
RDR6 is required for efficient hpRNA-induced RNA silencing in plants. PMID: 23456296
Genetic interactions during leaf development were observed in a similar way between miR396 overexpression and mutants in RDR6 PMID: 22889666
Compared with intronless transcripts, the spliced transcripts were less effective substrates for RNA-dependent RNA polymerase 6-mediated gene silencing. PMID: 21689169
These data suggest that AGO1 functions differentially with 21- and 22-nt miRNAs to engage the RDR6-associated amplification apparatus. PMID: 20562854
A recessive mutation in RDR6 causes enhanced self-incompatibility. PMID: 19767457
Arabidopsis RNA-Dependent RNA Polymerase (RDR6) gene regulates leaf development. [RDR6] PMID: 16006579
A hypomorphic rdr6 mutant, which does not exhibit the leaf developmental defects observed in null mutants, accumulates wild-type TAS3 ta-siRNA levels but not TAS1 and TAS2 ta-siRNAs, suggesting that TAS3 is required for proper leaf development. PMID: 16682354
Improperly terminated, unpolyadenylated mRNA from transgene transcription is subject to RDR6-mediated RNA silencing, probably by acting as templates for the RNA polymerase, in Arabidopsis thaliana. PMID: 17384170
Several gene transcripts were found to be routed through the RDR6/DCL4-dependent pathway after initial targeting by one or multiple microRNAs or trans-acting short interfering RNA. PMID: 17400893
Biochemical analysis of Arabidopsis RNA-dependent RNA polymerase 6 PMID: 18063577
AGO1 and AGO7 function to ensure efficient clearance of viral RNAs and establish that AGO1 is capable of targeting viral RNAs with more compact structures, whereas AGO7 and RDR6 favor less structured RNA targets PMID: 18799732

Hide All

Subcellular Location

Cytoplasmic granule. Nucleus.

Protein Families

RdRP family

Tissue Specificity

Widely expressed.

Database Links

KEGG: ath:AT3G49500

STRING: 3702.AT3G49500.1

UniGene: At.65263

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RDR6 Antibody

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