Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SEC14 Polyclonal antibody

Uniprot No.

P24280

Target Names

SEC14

Alternative Names

SEC14 antibody; PIT1 antibody; YMR079W antibody; YM9582.04 antibody; SEC14 cytosolic factor antibody; Phosphatidylinositol/phosphatidylcholine transfer protein antibody; PI/PC TP antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SEC14 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Required for transport of secretory proteins from the Golgi complex. Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes in vitro. Essential for viability and secretion. Exchanges its bound phospholipid with phospholipid monomers that reside in membrane bilayers. Regulates specific trans-Golgi export pathways, like transport from endosomes to the trans-Golgi or transport from the plasma membrane to the vacuole at the level of the endosome. Increased membrane curvature and lipid unsaturation levels at the trans-Golgi membrane promotes membrane binding and phospholipid transfer of SEC14. Thus, SEC14 may act at the trans-Golgi membrane to exchange lipids and promote vesicle formation. The phosphatidylcholine-bound form of SEC14 represses the CDP-choline pathway activity by inhibiting CCTase, the rate-determining enzyme of the CDP-choline pathway. PI binding/transfer is dispensable for function in vivo. Required for trafficking and localization of lipid raft proteins to the plasma membrane.

Gene References into Functions

Data suggest that SEC14L and SFH1 undergo conformational changes as phosphatidylinositol/phosphatidylcholine bind to hydrophobic pockets/ligand binding sites. [review-like article] PMID: 25233419
Sec14 and Sec14 like proteins form central conduits to integrate multiple aspects of lipid metabolism with productive phosphoinositide signaling. (Review) PMID: 22374094
The crystal structure of Sec14 family homologue (Sfh)3 is the first report of a dimeric Sec14 protein and provides clues for its functional study. PMID: 23519406
Localization of lipid raft proteins to the plasma membrane is a major function of the phospholipid transfer protein Sec14. PMID: 23383173
To launch a detailed structural and functional characterization of Sfh3, the recombinant protein was purified to homogeneity, diffraction-quality crystals were produced and a native X-ray data set was collected to 2.2 A resolution PMID: 22102037
Sec14 protein is a peripheral Golgi membrane protein essential for Golgi secretory function and cell viability[review]. PMID: 16118014
This review describes Sec14p, the major (phosphatidylinositol)/PC (phosphatidylcholine)-transfer protein in yeast, which co-ordinates lipid metabolism with protein transport from the Golgi complex. PMID: 16709158
The application of EPR spectrscopy to investigate the chemical properties of binding between phosphatidylcholine and Sec14 is reported. PMID: 16997918
molecular dynamics suggests large rigid body motions within Sec14p accompany closing and opening of an A(10)/T(4)/A(11) helical gate, and that "state-of-closure" of this helical gate determines access to the Sec14p phospholipid binding cavity PMID: 17344474
This review focuses on five yeast proteins that share significant sequence homology with Sec14p and have distinct subcellular localizations with functions related to lipid metabolism, phosphoinositide mediated signaling and membrane trafficking. PMID: 17395532
a regulatory role for Sec14 is a primary counter to action of the Kes1 sterol-binding protein that antagonizes PtdIns 4-OH kinase activity in vivo PMID: 18243114

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Subcellular Location

Golgi apparatus membrane; Peripheral membrane protein. Cytoplasm.

Database Links

KEGG: sce:YMR079W

STRING: 4932.YMR079W

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SEC14 Antibody

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