seqA Antibody

1. Data indicate that the SeqA protein is localized at midcell for most of the D-period, but colocalizes with the origin region before cell division and initiation of replication. PMID: 25722374
2. Data suggest that, in the absence of SeqA, the sister-chromatid cohesion 'safety spacer' is destabilized and completely disappears if the replication fork is inhibited. PMID: 24806348
3. We propose that SeqA binding results in loose inter-duplex junctions that are resistant to Topo IV cleavage PMID: 23990792
4. Cell cycle analysis showed that loss of SeqA from the old replication forks did not occur at initiation of the new forks, but instead occurs at a time point coinciding with the end of SeqA-dependent origin sequestration. PMID: 22373925
5. The number of SeqA foci is not directly governed by the number of replication forks, and supports the idea that new DNA may be 'captured' by existing SeqA structures. PMID: 19371375
6. well-defined SeqA structures are not found in situations of extensive replication fork repair PMID: 19898675
7. SeqA protein bound to fully methylated, covalently closed or nicked circular DNA generates positive supercoils in vitro in the presence of topoisomerase I or ligase respectively. PMID: 15458410
8. These results indicate that SeqA binds hemimethylated nascent DNA segments according to the proceeding of replication forks in the chromosome PMID: 15612935
9. Data report the crystal structure of the oligomerization domain of SeqA (SeqA-N). PMID: 15933720
10. Overproduction of SeqA4 led to partially restored initiation synchrony, indicating that origin sequestration may not depend on efficient higher-order multimerization into foci, but do require a high local concentration of SeqA. PMID: 16236133
11. These results suggest that for survival of replication fork damage, SeqA's repression of replication initiation is more important than its effects on nucleoid organization. PMID: 16556234
12. it has been demonstrated that two GATC sequences, located 82 and 105 bp downstream of the bacteriophage lambda p(R) transcription start site, are necessary for transcription stimulation by SeqA both in vivo and in vitro PMID: 17005979
13. investigation of how resetting of oriC to the ORC-like complex is coordinated with SeqA-mediated sequestration; during the sequestration period, SeqA repressed pre-RC assembly while ensuring resetting of E. coli ORC PMID: 17114060
14. These results indicate that unlike oriC, SeqA modulates lambda DNA replication indirectly, most probably by influencing the stability of the lambda replication complex and the transcriptional activation of ori lambda. PMID: 17464080
15. An examination of the molecular model of Escherichia coli SeqA protein, a negative regulator of chromosome DNA replication which prevents the overinitiation of replication. PMID: 18849124
16. seqA mutants have increased LPS phosphorylation and exhibit high levels of chromosomal fragmentation. PMID: 19432803

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KEGG: ecj:JW0674

STRING: 316385.ECDH10B_0752