skn-1 Antibody

Code CSB-PA339489XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans skn-1 Polyclonal antibody
Uniprot No.
Target Names
skn-1
Alternative Names
skn-1 antibody; T19E7.2 antibody; Protein skinhead-1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans skn-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor required to specify the fate of ventral blastomeres in the early embryo, and postembryonically for the development of the intestine. Directly regulates expression of zygotically expressed med-1 and med-2 to direct mesendoderm development. Required for stl-1 mRNA up-regulation in response to oxidative stress and anoxia. Required for the up-regulation of gcs-1 and several glutathione-S-transferase mRNAs in response to oxidative stress generated during pathogenic bacterial infection. Regulates the transcription of genes associated with metabolism in response to changes in nutrient availability. In neurons, involved in mitochondrial fusion and behavioral recovery during reoxygenation. Required for riok-1 mRNA expression in the intestine. Downstream of the let-60/Ras, mek-2 and pmk-1 pathway, positively regulates lifespan probably by preventing transcription of insulin-like peptides such as ins-39. Prevents degeneration of dopaminergic CEP neurons in response to high Al(3+) or Mn(2+) levels, probably by promoting the expression of glutathione-S-transferase gst-1.; Directed by the ER-associated degradation pathway (ERAD), mediates proteasomal homeostasis by regulating the expression of proteasomal subunits such as rpt-3 to confer resistance to proteasomal dysfunction.
Gene References into Functions
  1. SKN-1 is required along with ELT-3 for the oxidative stress response. SKN-1 interacts with ELT-3. PMID: 28600327
  2. DDI-1 expression is induced by proteasome dysfunction, and the authors show that DDI-1 is required to cleave and activate an endoplasmic reticulum-associated isoform of SKN-1. PMID: 27528192
  3. The peptides from sesame cake (PSC) up-regulated skn-1 mRNA, and its target gene gcs-1, and abolished the extension of lifespan in skn-1 mutant, indicating that PSC-mediated longevity is dependent on activation of the skn-1/Nrf-2 transcription factor. PMID: 27381188
  4. Findings show that Nrf2/skn-1 is expressed in interneurons involved in chemosensation in mice and C. elegans, and that genetic deletion of skn-1 results in impaired sensory integration. The inability to activate skn-1 signaling causes the skn-1 worms to linger in inadequate food patches rather than migrate toward new potential food sources. PMID: 28459841
  5. Our data also indicate that thioallyl structure and the number of sulfur atoms are important for SKN-1 target induction. Our results indicate that SAC and SAMC may serve as potential agents that slow aging PMID: 26899496
  6. SKN-1 plays a central role in diverse genetic and pharmacologic interventions that promote C. elegans longevity, suggesting that mechanisms regulated by SKN-1 may be of conserved importance in aging PMID: 26232625
  7. The rpn-10 mutant animals survive through the activation of compensatory mechanisms regulated by the conserved SKN-1/Nrf2 and ELT-2/GATA transcription factors that mediate the increased expression of genes encoding proteasome subunits. PMID: 26828939
  8. Data show that movements of two key arginine side chains between the major groove and the backbone of DNA generate distinct conformational sub-states that each recognize only part of the consensus binding sequence of transcription factor SKN-1. PMID: 26721385
  9. The authors conclude that SKN-1 plays a direct role in maintaining lipid homeostasis in which it is activated by lipids. PMID: 26196144
  10. SKN-1 and Nrf2 has a role in linking proline catabolism with lipid metabolism during nutrient deprivation PMID: 25284427
  11. PTL-1 functions via neurons to modulate SKN-1, clarifying the role of this protein in the stress response and longevity. PMID: 25399685
  12. A network analysis of aging-associated miRNAs connected to transcription factors PHA-4/FOXA and SKN-1/Nrf revealed extensive regulatory interactions between PHA-4, SKN-1, and miRNAs undergoing dietary restriction. PMID: 25242029
  13. Anoxia results in mitochondrial oxidative stress, and the oxidative response factor SKN-1/Nrf is required for both rapid mitochondrial refusion and rapid behavioral recovery during reoxygenation PMID: 24385935
  14. studies suggest that knockdown of skn-1 in the nematode increases DAergic sensitivity to MeHg exposure following a period of latency PMID: 24194349
  15. our results suggest that SKN-1 activation in the nervous system can confer protection to organisms in response to stress by directly regulating nlg-1/neuroligin expression. PMID: 24453991
  16. Regulatory integration through SKN-1/Nrf may coordinate ER and cytoplasmic homeostasis. PMID: 24068940
  17. genetic inactivation of rict-1 suppressed skn-1-associated lethality by restoring mesendodermal specification in skn-1 deficient embryos PMID: 23973804
  18. These results provide insight into how SKN-1/Nrf2 might protect the nervous system from damage in response to oxidative stress. PMID: 23555279
  19. SKN-1/Nrf functions in an evolutionarily conserved pathway to modulate metabolism and adaptation to starvation. PMID: 23040073
  20. Mutations in skn-1 and knockdown of genes encoding phase II detoxification enzymes led to increased sterility in the presence of dietary dihomo-gamma-linolenic acid. PMID: 23064027
  21. discussion of structure and function of SKN-1 in relation to drug resistance to anthelminthic agents; includes amino acid sequence alignment/homology and phylogeny [REVIEW] PMID: 22656429
  22. SKN-1 contributes to the enhanced oxidative stress resistance incurred by hcf-1 mutation PMID: 22568582
  23. SKN-1, as a novel regulator of innate immunity, involves in immunosenescence and provides an important crosstalk between pathogenic stress signaling and the xenobiotic/oxidative stress response. PMID: 22577361
  24. Mutation or silencing of WDR-46 activates the single C. elegans CnC homologue SKN-1 and increases expression of its target genes. PMID: 22240150
  25. a reduction in SKN-1 gene expression increases MeHg-induced animal vulnerability and dopamine neuron degeneration. PMID: 20855423
  26. SKN-1 detoxification gene network monitors various metabolic and regulatory processes. PMID: 20700440
  27. the ERK (MPK-1) cascade-mediated life span extension requires SKN-1, whose function is mediated, at least partly, through DAF-2/DAF-16 insulin-like signaling. MPK-1 phosphorylates SKN-1 on the key sites that are required for SKN-1 nuclear accumulation. PMID: 20624915
  28. Results show that cell stress triggers aip-1 expression by both skn-1-dependent and -independent pathways. PMID: 20351174
  29. SKN-1 represents a connection between developmental specification of the digestive system and resistance to oxidative and xenobiotic stress PMID: 12869585
  30. med transcripts accumulate both in the EMS cell and in the maternal germline in a SKN-1-dependent manner PMID: 16979152
  31. EEL01 controls asymmetry and persistence of Skn-1 in the early C. elegans embryo. PMID: 17537795
  32. increased longevity of diet-restricted Caenorhabditis elegans requires the transcription factor gene skn-1 acting in the ASIs, a pair of neurons in the head PMID: 17538612
  33. we have identified four kinases, MKK-4, IKK epsilon-1, NEKL-2, and PDHK-2, which are necessary for the nuclear localization of SKN-1 in response to oxidative stress PMID: 17964427
  34. The findings indicate that the transcription network regulated by SKN-1 promotes longevity and is an important direct target of Insulin/IGF-1-like signaling. PMID: 18358814
  35. Striking complexity in SKN-1 functions and the regulation of systemic detoxification defenses in Caenorhabditis elegans. PMID: 19575768
  36. findings showed that a transcriptional shift from growth and maintenance towards the activation of cellular defense mechanisms was caused by the oxidative stress; many of these transcriptional alterations are SKN-1 dependent. PMID: 19627265

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Subcellular Location
Nucleus. Cytoplasm.; [Isoform a]: Nucleus. Mitochondrion.
Protein Families
BZIP family, Skn1 subfamily
Tissue Specificity
Postembryonic intestinal cells.
Database Links
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