Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SNF7 Polyclonal antibody

Uniprot No.

P39929

Target Names

SNF7

Alternative Names

SNF7 antibody; DID1 antibody; VPS32 antibody; YLR025WVacuolar-sorting protein SNF7 antibody; DOA4-independent degradation protein 1 antibody; Sucrose nonfermenting protein 7 antibody; Vacuolar protein-sorting-associated protein 32 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SNF7 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Acts a component of the ESCRT-III complex required for the sorting and concentration of proteins resulting in the entry of these proteins into the invaginating vesicles of the multivesicular body (MVB). The sequential action of ESCRT-0, -I, and -II together with the ordered assembly of ESCRT-III links membrane invagination to cargo sorting. Membrane scission in the neck of the growing vesicle releases mature, cargo-laden ILVs into the lumen. ESCRT-III is critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. SNF7 is the most abundant ESCRT-III subunit which forms membrane-sculpting filaments with 30 Angstrom periodicity and a exposed cationic membrane-binding surface. Its activation requires a prominent conformational rearrangement to expose protein-membrane and protein-protein interfaces. SNF7 filaments then form spirals that could function as spiral springs. The elastic expansion of compressed SNF7 spirals generates an area difference between the two sides of the membrane and thus curvature which could be the origin of membrane deformation leading eventually to fission. SNF7 recruits BRO1, which in turn recruits DOA4, which deubiquitinates cargos before their enclosure within MVB vesicles. ESCRT-III is also recruited to the nuclear envelope (NE) by integral INM proteins to surveil and clear defective nuclear pore complex (NPC) assembly intermediates to ensure the fidelity of NPC assembly.

Gene References into Functions

The major ESCRT-III subunit Snf7 localizes transiently to vesicles and tubules surrounded by a cup-shaped membrane and this was accelerated in vps4Delta cells, correlating with increased Acb1 secretion. PMID: 27115345
Novel mutations has been characterized in ESCRT-III Snf7 that trigger activation, and a novel role have been assigned to Bro1, the yeast ALIX ortholog, in Snf7 assembly. PMID: 27074665
Using a combination of biochemical and genetic approaches, both in vitro and in vivo, we demonstrate that mutations on these protein interfaces halt Snf7 assembly and block endosomal sorting complexes required for transport function. PMID: 26670543
Snf7 alpha6 helix is crucial for binding of Bro1 and Rim20 in vivo and is indispensable for the multivesicular body sorting. PMID: 24058170
Binding of Vps4 to ESCRT-III, coordinated by interactions with Vps2 and Snf7, is coupled to membrane neck constriction during intraluminal vesicle formation. PMID: 24711499
we demonstrate that the N terminus of Snf7 has been tuned to maintain the topological constraints associated with ESCRT-III-filament-mediated membrane invagination and vesicle formation. PMID: 24139821
A rns4/vps32 mutation on the RNase T1 expression-sensitive strain of Saccharomyces Cerevisiae provides evidence for altered ambient response resulting in transportation of the secretory protein to vacuoles. PMID: 15925308
Snf7 influences SUC2 gene expression through activation of the Rim101 pathway. PMID: 18806212
By generating SNF7 mutations that severely affect endocytic trafficking, but leave the Rim pathway function intact, data show that the two functions of SNF7 can be separated genetically. PMID: 19725809

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Subcellular Location

Cytoplasm. Endosome membrane; Peripheral membrane protein. Nucleus envelope.

Protein Families

SNF7 family

Database Links

KEGG: sce:YLR025W

STRING: 4932.YLR025W

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SNF7 Antibody

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