Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) smpB Polyclonal antibody

Uniprot No.

P0A832

Target Names

smpB

Alternative Names

smpB antibody; smqB antibody; b2620 antibody; JW2601 antibody; SsrA-binding protein antibody; Small protein B antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) smpB protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Required for rescue of stalled ribosomes mediated by trans-translation. Binds to tmRNA RNA (also known as SsrA or 10Sa RNA, 363 nucleotides in this organism), required for stable binding of tmRNA to ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB (Probable). tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. Able to recruit charged tmRNA to ribosomes. Does not play a role in transcription, processing or Ala-aminoacylation of tmRNA. Other studies have shown it stimulates aminoacylation of tmRNA. May protect tmRNA from degradation. Binds to tmRNA that cannot be aminoacylated (tmRNA G3A), does not bind to tmRNA mutations near the tRNA-like termini (tmRNA G19C, A334U); other tmRNA mutations that block trans-translation still bind SmpB. With tmRNA may play a role in bacterial persistence. During trans-translation Ala-aminoacylated transfer-messenger RNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA, the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.

Gene References into Functions

These data indicate that the tmRNA-SmpB system is important in the pathogenesis of Avian pathogenic E. coli O2 strain E058. PMID: 24013628
Transfer-messenger RNA-SmpB protein regulates ribonuclease R turnover by promoting binding of HslUV and Lon proteases PMID: 22879590
cryo-electron microscopy reconstruction of a fusidic-acid-stalled ribosomal 70S-tmRNA-SmpB-EF-G complex (carrying both of the large ligands, that is, EF-G and tmRNA) at 8.3 A resolution PMID: 22622583
There is tight and specific binding of SmpB to transfer messenger (tm)RNA. Surprisingly, the global conformation and flexibility of tmRNA do not change upon SmpB binding, as a function of magnesium ion (Mg2+) concentration. PMID: 22482838
Mutations in the SmpB tail reduce rates of peptidyl transfer to tmRNA, but not GTP hydrolysis by EF-Tu. PMID: 21795410
Data conclude that the tmRNA.SmpB complex accommodates in the ribosomal A site very much like an aminoacyl-tRNA during protein elongation. PMID: 19883769
truncation of the C-terminal tail or substitution of tryptophan residue at 147 in the middle of the C-terminal tail affected the activity in the early stage of trans-translation PMID: 20348441
protein SmpB enhanced transfer-messenger RNA (tmRNA) processing, suggesting a new role for SmpB in trans-translation PMID: 15494393
SmpB remains tmRNA bound at the initial steps of trans-translation PMID: 17400816
These results suggest that SmpB serves to bridge two separate domains of tmRNA to determine the initial codon for tag-translation. PMID: 17698641
within a stalled ribosome, SmpB interacts with the three universally conserved bases G530, A1492 and A1493 that form the 30S subunit decoding centre, in which canonical codon-anticodon pairing occurs PMID: 19132006
These results demonstrate that SmpB is functionally linked with the sequence upstream of the tmRNA template; both contribute to reading frame selection on tmRNA. PMID: 19540849

Hide All

Subcellular Location

Cytoplasm.

Protein Families

SmpB family

Database Links

KEGG: ecj:JW2601

STRING: 316385.ECDH10B_2786

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smpB Antibody

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