Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) STU2 Polyclonal antibody

Uniprot No.

P46675

Target Names

STU2

Alternative Names

STU2 antibody; YLR045C antibody; L2108Protein STU2 antibody; Suppressor of tubulin 2 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) STU2 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. May play a role in the attachment, organization, and/or dynamics of microtubule ends at the spindle pole body. Stabilizes both cytoplasmic and nuclear microtubules. Promotes mitotic spindle elongation in anaphase. Has microtubule polymerase activity by accelerating microtubule growth and inhibiting catastrophe. The polymerase activity is proposed to involve a tethering mechanism at the microtubule plus end: a curved, longitudinally microtubule lattice-associated tubulin heterodimer (that cannot be incorporated into the microtubule lattice) is bound via one TOG domain while the other TOG domain binds to an unpolymerized tubulin heterodimer leading to lateral association of these tubulin heterodimers; polymerization-induced straightening of the tubulin heterodimer releases the polymerase. Is involved in regulation of kinetochore-microtubule attachments in a tension-dependent manner involving its association with the NDC80 complex; the function may be independent of its microtubule polymerase activity. Can either stabilize or destabilize kinetochore attachments, depending on the level of kinetochore tension and whether the microtubule tip is assembling or disassembling.

Gene References into Functions

TORC1/Kog1 signaling controls Stu2 localization and thereby contributes to proper microtubule cytoskeletal organization in interphase and mitosis. PMID: 28972103
findings reveal an uncharacterized function of Stu2 that is regulated mechanically, such that kinetochore-microtubule attachments are intrinsically stabilized by tension, implicating it in the correction of erroneous kinetochore-microtubule attachments PMID: 27156448
Stu2p/XMAP215 proteins are essential microtubule polymerases that use multiple alpha-beta-tubulin-interacting TOG domains to bind microtubule plus ends and catalyze fast microtubule growth. PMID: 25097237
Stu2 is a Saccharomyces cerevisiae microtubule polymerase. PMID: 25172511
anaphase Stu2-dependent microtubule dynamics are critical for separation of long chromosomes. PMID: 25225337
The microtubule rescue distal to the kinetochore is also promoted by Stu2, which is transported by a kinesin-8 motor Kip3 along the microtubule from the kinetochore. PMID: 22075150
Stu2p binds to a single free tubulin heterodimer through its first TOG domain and has the capacity to associate directly with microtubule ends, at least in part, through its second TOG domain. PMID: 16567500
Mislocalization of Stu2 results in premature spindle expansion in S phase stalled spc24-9 mutants. PMID: 17507656
Crystal structures of the tubulin binding domains of XMAP215 (yeast Stu2p and Drosophila Msps), EB1 (yeast Bim1p and human EB1), and CLIP-170 (human), which reveal diverse tubulin binding interfaces, are reported. PMID: 17889670

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Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore.

Protein Families

TOG/XMAP215 family

Database Links

KEGG: sce:YLR045C

STRING: 4932.YLR045C

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STU2 Antibody

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