SVP Antibody

Code CSB-PA880830XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SVP Polyclonal antibody
Uniprot No.
Target Names
SVP
Alternative Names
SVP antibody; At2g22540 antibody; F14M13.6 antibody; MADS-box protein SVP antibody; Protein SHORT VEGETATIVE PHASE antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SVP protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription repressor that inhibit floral transition in the autonomous flowering pathway, independent of photoperiod and temperature. Acts in a dosage-dependent manner. Together with AGL24 and AP1, controls the identity of the floral meristem and regulates expression of class B, C and E genes. Promotes EFM expression to suppress flowering.
Gene References into Functions
  1. these results reveal a novel role for the floral regulators SVP and SOC1 in disease resistance and provide evidence that salicylic acid acts directly on pathogens as an antimicrobial agent. PMID: 28812948
  2. The data suggests that AGL22 uniquely regulates a transcriptional network during drought stress, linking changes in primary metabolism and the initiation of stress responses. PMID: 26842464
  3. SVP protein up-regulates TEM2 floral repressor at low temperatures.SVP protein accumulation is higher during the day than during the night under long day conditions. PMID: 26243615
  4. Data show that microRNA miR396 triggers SHORT VEGETATIVE PHASE (SVP) mRNA decay rather than miRNA-mediated cleavage. PMID: 26103992
  5. The downstream targets of the SVP:FLC complex include a higher proportion of genes regulating floral induction, whereas those bound by either TF independently are biased towards floral development. PMID: 25853185
  6. In brm mutants, elevated PcG occupancy at SVP accompanies a dramatic increase in H3K27me3 levels at this locus and a concomitant reduction of SVP expression. PMID: 25615622
  7. post-translational regulation of SVP functions as a major mechanism for thermoregulation in flowering PMID: 24614351
  8. SVP regulates many developmental pathways, some of which are common to both of vegetative and reproductive development whereas others are specific to only one of them. PMID: 23759218
  9. The sequential formation of FUL-SVP and FUL-SOC1 heterodimers may mediate the vegetative and meristem identity transitions, counteracting the repressive effect of FLC and SVP on flowering. PMID: 24465009
  10. SVP delays flowering by repressing gibberellin biosynthesis as well as integrator gene expression. PMID: 24979809
  11. Control of SVP-FLM-beta repressor complex abundance via transcriptional and splicing regulation of FLM and posttranslational regulation of SVP protein stability provides an efficient, rapid mechanism for plants to respond to ambient temperature changes. PMID: 24030492
  12. These results support that the flowering repressor SVP has been recently selected in A. thaliana as a target for early flowering, and evidence the relevance of genetic interactions for the intraspecific evolution of FAQ1/SVP and flowering time. PMID: 23382706
  13. SVP protein directly regulates miR172 transcription in Arabidopsis PMID: 22659182
  14. Findings show that feedback regulatory loops mediated by SOC1 and SVP are essential components of the gene regulatory networks that underpin the integration of flowering signals during floral transition. PMID: 22268548
  15. LHY/CCA1 regulates a pathway negatively controlling flowering locus T (FT), possibly via ELF3-SVP/FLC. PMID: 19383102
  16. SVP negatively regulates TSF as well as FT. PMID: 19656342
  17. AGL24, AP1 and SVP directly and redundantly regulate class B, C and E floral homeotic genes. PMID: 19656343
  18. The data provides genetic evidence for the role of AP1 in these interactions by showing that the floral phenotype in the ap1 agl24 svp triple mutant is significantly enhanced. PMID: 16679456
  19. SVP mediates the temperature-dependent functions of FCA and FVE within the thermosensory pathway. PMID: 17322399
  20. once AP1 is activated during the floral transition, it acts partly as a master repressor in floral meristems by directly suppressing the expression of flowering time genes SVP, AGL24 and SOC1, preventing continuation of the shoot developmental program PMID: 17428825
  21. Complementation tests showed that rice OsMADS22 and OsMADS47 are unable to reverse the flowering-time phenotypes of the svp and agl24 mutants in Arabidopsis. PMID: 18453531
  22. AP1, AGL24 and SVP redundantly control floral meristem identity. Ectopic AGL24 and SVP expression promotes floral indeterminacy. PMID: 18694458
  23. SVP protein accumulation is regulated by LHY and CCA1. Reduction of SVP leads to accelerated flowering time. PMID: 19011118

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Subcellular Location
Nucleus.
Tissue Specificity
Detected in roots and leaves. Expressed at very low levels in flowers and siliques. Present in floral meristems.
Database Links

KEGG: ath:AT2G22540

STRING: 3702.AT2G22540.1

UniGene: At.22081

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