Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TRX1 Polyclonal antibody

Uniprot No.

P22217

Target Names

TRX1

Alternative Names

TRX1 antibody; TRX2 antibody; YLR043C antibody; Thioredoxin-1 antibody; Thioredoxin I antibody; TR-I antibody; Thioredoxin-2 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TRX1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Participates as a hydrogen donor in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, accompanied by the transfer of 2 electrons and 2 protons. It is involved in many cellular processes, including deoxyribonucleotide synthesis, repair of oxidatively damaged proteins, protein folding, sulfur metabolism, and redox homeostasis. Thioredoxin-dependent enzymes include phosphoadenosine-phosphosulfate reductase MET16, alkyl-hydroperoxide reductase DOT5, thioredoxin peroxidases TSA1 and TSA2, alkyl hydroperoxide reductase AHP1, and peroxiredoxin HYR1. Thioredoxin is also involved in protection against reducing stress. As part of the LMA1 complex, it is involved in the facilitation of vesicle fusion such as homotypic vacuole and ER-derived COPII vesicle fusion with the Golgi. This activity does not require the redox mechanism.

Gene References into Functions

NMR spectroscopic studies of yeast TRX1 provide insights into the mechanism for the closing of the water cavity. PMID: 26482062
TRX1 is required to maintain the IMS small Tim proteins in reduced forms and facilitate their mitochondrial import PMID: 22878414
Results show that an oxidized sulfiredoxin under disulfide state is formed between the catalytic Cys(84) and Cys(48), and this oxidized is reduced selectively by thioredoxin. PMID: 19801666
The cytosolic form of thioredoxin Trx1 has been cloned, expressed, purified and crystallized. PMID: 18391437
Human wt p53 expression in yeast leads to gene repression of thioredoxin (TRX1/2), a highly conserved multifunctional antioxidative and antiapoptotic protein family. PMID: 19054132
X-ray diffraction analysis of glutathionylated Trx1 C33S mutant from yeast PMID: 19153453
study solved the crystal structures of oxidized Trx1 and glutathionylated Trx1Cys33Ser mutant at 1.76 and 1.80 A, respectively; kinetic studies proved that Met35Arg mutation could alter the apparent K(m) and V(max) values of the reaction PMID: 19362171
Trx1 and Trx2 are essential for the nuclear accumulation of Msn2/4 and Maf1. PMID: 19581440

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Subcellular Location

Nucleus. Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Mitochondrion intermembrane space.

Protein Families

Thioredoxin family

Database Links

KEGG: sce:YLR043C

STRING: 4932.YLR043C

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TRX1 Antibody

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