Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TSA1 Polyclonal antibody

Uniprot No.

P34760

Target Names

TSA1

Alternative Names

TSA1 antibody; TPX1 antibody; TSA antibody; ZRG14 antibody; YML028W antibody; Peroxiredoxin TSA1 antibody; Prx antibody; EC 1.11.1.15 antibody; Cytoplasmic thiol peroxidase 1 antibody; cTPx 1 antibody; Protector protein antibody; PRP antibody; Thiol-specific antioxidant protein 1 antibody; Thioredoxin peroxidase type Ia antibody; TPx type Ia antibody; Thioredoxin-dependent peroxide reductase antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TSA1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Protects the cell against the oxidative stress caused by nascent-protein misfolding and aggregation. Relays hydrogen peroxide as a signal to the transcription factor YAP1 by inducing the formation of intramolecular disulfide bonds in YAP1, which causes its nuclear accumulation and activation. Can act alternatively as peroxidase and molecular chaperone. Oxidative stress and heat shock exposure cause a reversible shift of the protein structure from low MW species to high MW complexes, triggering a peroxidase-to-chaperone functional switch. The chaperone function of the protein enhances resistance to heat shock.

Gene References into Functions

The critical molecular event allowing the Tsa1 to chaperone switch is the enzyme assembly into high molecular weight structures brought about by enzyme hyperoxidation and sulfinylation. PMID: 26335398
Tsa1 localises to the sites of protein aggregation and protects against protein-aggregate-induced oxidative stress. PMID: 24424024
It appears to control the cellular reactive oxygen species. PMID: 24418709
Tsa1, the major peroxiredoxin, is required for telomere length maintenance. PMID: 23590194
Glu50 and Arg146 are important for the Tsa1-Trx interaction. These results may impact the comprehension of downstream events of signaling pathways that are triggered by the oxidation of critical Cys residues, such as Trx PMID: 22985967
Tsa1 is required to ensure yeast longevity and that caloric restriction extends yeast life span, in part, by counteracting age-induced hyperoxidation of this peroxiredoxin PMID: 21884982
TSA1-B7 encodes for a novel dominant form of peroxiredoxin, and establishes a new connection between oxidative stress and aging PMID: 20729566
An elevation in intracellular dNTP production was observed in tsa1 mutants. PMID: 19851444
Tsa1 normally functions to chaperone misassembled ribosomal proteins, preventing the toxicity that arises from their aggregation PMID: 16251355
Results suggest that cytosolic thioredoxin peroxidase I is specifically important in the protection of yeast with mitochondrial dysfunction due to its functional versatility as an antioxidant, chaperone and modulator of gene expression. PMID: 16441666
Implications for the role of peroxiredoxins in stress and growth control. PMID: 18271751
Expression of either Tsa1 or PrxI prevented Bax-induced cell death. These data indicate that PrxI is an orthologue of Tsa1. PrxI and Tsa1 seem to act on the same substrates in vivo and share similar mechanisms of function. PMID: 18281480
hyperoxidized Prx, Tsa1p-SO(3)H, with enhanced molecular chaperone activity and suggest that Tsa1p-SO(3)H is a marker of cumulative oxidative stress in cells PMID: 18725414
Tsa1 can interact with Yap1 via disulfide linkages and induce the formation of intramolecular disulfide bonds in Yap1 in ybp1-1 cells. PMID: 19106090
Tsa1 acts to protect S. cerevisiae cells against toxic levels of DNA damage. PMID: 19543365

Hide All

Subcellular Location

Cytoplasm.

Protein Families

Peroxiredoxin family, AhpC/Prx1 subfamily

Database Links

KEGG: sce:YML028W

STRING: 4932.YML028W

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA335979XA01SVG
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

TSA1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background