recG Antibody

Code CSB-PA326126ZA01ENV
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Product Details

Full Product Name Rabbit anti-Escherichia coli (strain K12) recG Polyclonal antibody
Uniprot No. P24230
Target Names recG
Alternative Names recG antibody; radC antibody; b3652 antibody; JW3627 antibody; ATP-dependent DNA helicase RecG antibody; EC 3.6.4.12 antibody
Raised in Rabbit
Species Reactivity Escherichia coli
Immunogen Recombinant Escherichia coli recG protein
Immunogen Species Escherichia coli (strain K12)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (12-14 weeks)

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Target Background

Function
(From Uniprot)
Plays a critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA). Has a role in constitutive stable DNA replication (cSDR) and R-loop formation. Is genetically synergistic to RadA and RuvABC.
Gene References into Functions
  1. results provide mechanistic insights into the modes of interaction of RecG with the replication fork, suggesting a novel role of RecG in the repair of stalled DNA replication forks. PMID: 29432678
  2. Data suggest that RecG and RuvABC catalyze alternative steps in DNA repair/recombination and collaborate to stabilize DNA during repair of double-strand DNA breaks; RecG may prevent DNA amplification at D-loops formed by recombination and at arrested replication forks. (RecG = RecG DNA helicase; Ruv = Holliday junction DNA helicase subunit) [REVIEW] PMID: 28155219
  3. interaction occurs via the wedge domain of RecG and the intrinsically disordered linker (IDL) of SSB, in a manner similar to that of SH3 domains binding to PXXP motif-containing ligands in eukaryotic cells. PMID: 28078722
  4. Once RecG has regressed the fork, it will dissociate leaving the now relaxed, Holliday junction-like DNA, available for further processing by enzymes such as RuvAB. [review] PMID: 25613916
  5. RecG co-localises with sites of DNA replication in Escherichia coli. PMID: 24692661
  6. Abolishing PriB leads to suppression of the recG null phenotype. PMID: 22957744
  7. data are consistent with RecG and ssDNA exonucleases acting to limit PriA-mediated re-replication of the chromosome and the consequent generation of linear DNA branches that provoke recombination and delay chromosome segregation PMID: 20647503
  8. The authors show that the extensive DnaA-independent stable DNA replication observed without RecG can lead to replication of any area of the chromosome. PMID: 19818016
  9. allows repair of damaged replication forks to proceed without recourse to potentially mutagenic recombination PMID: 15516585
  10. Data suggest that the processivity of RecG on branched DNA substrates is dependent on the ability of its wedge domain to establish strong binding at the branch point. PMID: 15695524
  11. Inactivation of recG stimulates recombinant formation about five-fold PMID: 16257588
  12. manner and timing of the interaction of RecG with DNA at stalled replication forks PMID: 17292398
  13. RecG decreases the number of replication forks in UV-irradiated cells by limiting UV-induced, DnaA-independent initiation of replication. PMID: 19538444

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Protein Families Helicase family, RecG subfamily
Database Links

KEGG: ecj:JW3627

STRING: 316385.ECDH10B_3834

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