Name: recG Antibody
Brand: CUSABIO
SKU: CSB-PA326126ZA01ENV

Product Details

Full Product Name	Rabbit anti-Escherichia coli (strain K12) recG Polyclonal antibody
Uniprot No.	P24230
Target Names	recG
Alternative Names	recG antibody; radC antibody; b3652 antibody; JW3627 antibody; ATP-dependent DNA helicase RecG antibody; EC 3.6.4.12 antibody
Raised in	Rabbit
Species Reactivity	Escherichia coli
Immunogen	Recombinant Escherichia coli recG protein
Immunogen Species	Escherichia coli (strain K12)
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	Protein A/G
Concentration	It differs from different batches. Please contact us to confirm it.
Buffer	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form	Liquid
Tested Applications	ELISA, WB (ensure identification of antigen)
Protocols	ELISA Protocol
Troubleshooting and FAQs	Antibody FAQs
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables	① 200ug * antigen (positive control); ② 1ml * Pre-immune serum (negative control);
Quality Guarantee	① Antibody purity can be guaranteed above 90% by SDS-PAGE detection; ② ELISA titer can be guaranteed 1: 64,000; ③ WB validation with antigen can be guaranteed positive;
Lead Time	Made-to-order (12-14 weeks)

Target Background

Function (From Uniprot)	Plays a critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA). Has a role in constitutive stable DNA replication (cSDR) and R-loop formation. Is genetically synergistic to RadA and RuvABC.
Gene References into Functions	results provide mechanistic insights into the modes of interaction of RecG with the replication fork, suggesting a novel role of RecG in the repair of stalled DNA replication forks. PMID: 29432678 Data suggest that RecG and RuvABC catalyze alternative steps in DNA repair/recombination and collaborate to stabilize DNA during repair of double-strand DNA breaks; RecG may prevent DNA amplification at D-loops formed by recombination and at arrested replication forks. (RecG = RecG DNA helicase; Ruv = Holliday junction DNA helicase subunit) [REVIEW] PMID: 28155219 interaction occurs via the wedge domain of RecG and the intrinsically disordered linker (IDL) of SSB, in a manner similar to that of SH3 domains binding to PXXP motif-containing ligands in eukaryotic cells. PMID: 28078722 Once RecG has regressed the fork, it will dissociate leaving the now relaxed, Holliday junction-like DNA, available for further processing by enzymes such as RuvAB. [review] PMID: 25613916 RecG co-localises with sites of DNA replication in Escherichia coli. PMID: 24692661 Abolishing PriB leads to suppression of the recG null phenotype. PMID: 22957744 data are consistent with RecG and ssDNA exonucleases acting to limit PriA-mediated re-replication of the chromosome and the consequent generation of linear DNA branches that provoke recombination and delay chromosome segregation PMID: 20647503 The authors show that the extensive DnaA-independent stable DNA replication observed without RecG can lead to replication of any area of the chromosome. PMID: 19818016 allows repair of damaged replication forks to proceed without recourse to potentially mutagenic recombination PMID: 15516585 Data suggest that the processivity of RecG on branched DNA substrates is dependent on the ability of its wedge domain to establish strong binding at the branch point. PMID: 15695524 Inactivation of recG stimulates recombinant formation about five-fold PMID: 16257588 manner and timing of the interaction of RecG with DNA at stalled replication forks PMID: 17292398 RecG decreases the number of replication forks in UV-irradiated cells by limiting UV-induced, DnaA-independent initiation of replication. PMID: 19538444 Show More Hide All
Protein Families	Helicase family, RecG subfamily
Database Links	KEGG: ecj:JW3627 STRING: 316385.ECDH10B_3834

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recG Antibody

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Code	CSB-PA326126ZA01ENV
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