Biotin ELISA Kit

Code CSB-E16209
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation Biotin
Protein Biological Process 1 others
Target Name N/A
Sample Types serum, plasma
Detection Range 12.5 pg/mL-200 pg/mL
Sensitivity 4.986 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive


Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.




To assess the linearity of the assay, samples were spiked with high concentrations of Biotin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


Typical Data


These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


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Q&A and Customer Reviews


Would you pls help to provide the following information regarding Biotin ELISA Kit(CSB-E16209):
1. What is the standard?
2. What is the antibody in this kit?

Thanks for your inquiry.
The standard is purified biotin;
This is a competitive kit and there is only mouse monoclonal antibody in the kit.
Pls let me know if you have any further questions. Thank you.
Customer Reviews
  • Tested application: ELISA
    Test sample: Serum
    Species: Human
    Review: To check biotin level in normal samples, I bought your kits and tested. I found out some samples were very high biotin level so I trace the clinical information of samples. It was very helpful to my research because biotin can make interference on my system.

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