Biotin ELISA Kit

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Citations (2) Protocol

Code	CSB-E16209
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (2)
Customer Reviews and Q&A

Product Details

Target Name

N/A

Abbreviation

Biotin

Sample Types

serum, plasma from human, mouse and other species

Detection Range

12.5 pg/mL-200 pg/mL

Sensitivity

4.986 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Biotin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Citations

Determination of Biotin Interference in Pediatric Obesity Related ELISA Research Kits Biotin Interference in Manual ELISA Kits E Kürkçü Kahraman, O Donma,Journal of Clinical Laboratory Analysis,2025
Prospective Analytical Controlled Study Evaluating Serum Biotin, Vitamin B12, and Folic Acid in Patients with Premature Canities Daulatabad D.et al,Int J Trichology,2017

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Sample type: Protein (recombinant/purified)

Sample species: Human

Sample dilution: 1:100

Review: I used biotin Elisa kit mainly for the detection of purified protein containing biotin. After experimental detection, it was found that the standard curve met the requirements, R2 was greater than 0.98, and the protein concentration after the standard curve was in line with expectations. The instructions for using the kit were accurate and clear.

By Anonymous

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■ Q&A

Would you pls help to provide the following information regarding Biotin ELISA Kit(CSB-E16209):
1. What is the standard?
2. What is the antibody in this kit?

Thanks for your inquiry.
The standard is purified biotin；
This is a competitive kit and there is only mouse monoclonal antibody in the kit.
Pls let me know if you have any further questions. Thank you.

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