Bovine Beta-Hydroxybutyric Acid,β-OHB ELISA Kit

Code CSB-E10056b
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name Beta-Hydroxybutyric Acid,β-OHB
Abbreviation β-OHB
Species Bos taurus (Bovine)
Sample Types serum, plasma
Detection Range 50 nmol/mL-800 nmol/mL
Sensitivity 9.5 nmol/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-OHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 88-95  
1:2 Average % 96  
Range % 91-103  
1:4 Average % 98  
Range % 92-104  
1:8 Average % 95  
Range % 89-100  
The recovery of bovine β-OHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 90-103  
EDTA plasma (n=4) 95 90-100  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nmol/ml OD1 OD2 Average    
800 0.136 0.138 0.137    
400 0.309 0.306 0.308    
200 0.674 0.682 0.678    
100 1.470 1.475 1.473    
50 2.706 2.712 2.709    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1