Bovine Interferon-Tau ELISA Kit

Code CSB-E16948B
Size 96T,5×96T,10×96T
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Product Details

Target Name
Interferon-Tau
Alternative Names
IFNT1 ELISA Kit; Interferon tau-1 ELISA Kit; IFN-tau-1 ELISA Kit; Antiluteolysin ELISA Kit; Trophoblast antiluteolytic protein ELISA Kit; Trophoblast protein 1 ELISA Kit; TP-1 ELISA Kit; Trophoblastin ELISA Kit
Abbreviation
IFN-tau
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine interferon-tau in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %82-92
1:2Average %98
Range %94-101
1:4Average %92
Range %87-98
1:8Average %95
Range %87-98
Recovery
The recovery of bovine interferon-tau spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-96
EDTA plasma (n=4)9691-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.587 2.558 2.573 2.485
2002.140 2.003 2.072 1.984
1001.644 1.621 1.633 1.545
501.109 1.027 1.068 0.980
250.698 0.687 0.693 0.605
12.50.399 0.387 0.393 0.305
6.250.242 0.231 0.237 0.149
00.090 0.086 0.088  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine IFN-tau ELISA Kit was designed for the quantitative measurement of Bovine IFN-tau protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

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Target Background

Function
(From Uniprot)
Paracrine hormone primarily responsible for maternal recognition of pregnancy. Interacts with endometrial receptors, probably type I interferon receptors, and blocks estrogen receptor expression, preventing the estrogen-induced increase in oxytocin receptor expression in the endometrium. This results in the suppression of the pulsatile endometrial release of the luteolytic hormone prostaglandin F2-alpha, hindering the regression of the corpus luteum (luteolysis) and therefore a return to ovarian cyclicity. This, and a possible direct effect of IFN-tau on prostaglandin synthesis, leads in turn to continued ovarian progesterone secretion, which stimulates the secretion by the endometrium of the nutrients required for the growth of the conceptus. In summary, displays particularly high antiviral and antiproliferative potency concurrently with particular weak cytotoxicity, high antiluteolytic activity and immunomodulatory properties. In contrast with other IFNs, IFN-tau is not virally inducible.
Gene References into Functions
  1. Interferon tau secreted from Day-7 embryo in vivo generates anti-inflammatory immune response in the bovine uterus, which may provide immunological tolerance to accept the embryo. PMID: 29702095
  2. The data suggest that TEAD relocation and/or YAP degradation following its phosphorylation down-regulates IFNT gene transcription after conceptus attachment to the uterine endometrium. PMID: 27315596
  3. Report interferon-tau dependent STAT1 regulation of SOCS genes in bovine endometrium during estrus cycle and embryo implantation. PMID: 25116692
  4. Suggest the involvement of IFNT function within the corpus luteum in the establishment of pregnancy in cows. PMID: 26078085
  5. These results indicate that HB-EGF and its receptors expression changed in bovine endometrium throughout the oestrous cycle; IFN-tau increased their expression in cultured endometrial cells. PMID: 25779761
  6. Data suggest exposure to maternal CSF2 from D5-D7 of development is fundamentally different for female/male blastocysts with respect to embryo elongation, characteristics of transcriptome/methylome, and endometrial interferon tau secretion at D15. PMID: 25078682
  7. as conceptuses attach to the uterine epithelium, IFNT gene transcription is down-regulated by an increase in EOMES expression and EOMES-CREBBP binding in the attached trophoblast cells. PMID: 23606646
  8. Heat shock increased both HSP70 and IFNT expression in blastocysts. PMID: 23638875
  9. The aims of this study were to identify the earliest transcriptional response of the bovine endometrium to the presence of the conceptus and investigate if these genes are regulated by interferon tau (IFNT). PMID: 22759920
  10. Bos taurus (B. T.) Coreanae IFN-tau was cloned from peripheral blood mononuclear cells. The amino acid sequence of B. T. Coreanae IFN-tau shares only 90.3% identity with that of Holstein dairy cow. PMID: 22578803
  11. activity in the early pregnancy endometrium in cattle is modulated by cortisol PMID: 22361386
  12. In a cell culture experiment, interferon-tau stimulated stimulated IL-10 mRNA expression in mononuclear leukocytes and tumor necrosis factor-alpha mRNA levels in neutrophils and eosinophils. PMID: 22052007
  13. In contrast to previous studies in the ovine, granulocyte-macrophage colony-stimulating factor did not increase interferon tau secretion but in agreement with the ovine did not affect bovine blastocyst development. PMID: 20977503
  14. Differentially expressed genes in the endometrial transcriptome are a consequence of IFNT production by the conceptus. PMID: 21349821
  15. The increase in calving rate caused by CSF2 treatment involves, in part, more extensive development of extraembryonic membranes and capacity of the conceptus to secrete IFNT2 at day 15 of pregnancy. PMID: 21339286
  16. investigation of signaling mechanism used by fibroblast growth factor-2 (FGF2) to regulate IFNT production in trophoblasts: several lines of evidence suggest that FGF2 regulates IFNT production in trophoblasts by acting through protein kinase C-delta PMID: 21191110
  17. These data suggest that GATA2/3 is involved in trophoblast-specific regulation of bovine intereron tau gene transcription. PMID: 19598245
  18. During pregnancy, interferon-tau may block ERalpha in the luminal epithelium but likely does not rescue integrin alphavbeta3. expression. PMID: 12756058
  19. Importance of complex Ets-2 enhancer for expression of interferon-tau. Possible means whereby the mother can exert control over conceptus IFN-tau production. PMID: 15217985
  20. The increase in interferon tau concentrations responsible for the maternal recognition of pregnancy results from the increase in embryo size during elongation and not from any upregulation of mRNA expression. PMID: 16435375
  21. FGF-2 stimulates IFNT expression and plays an active role in regulating the establishment and maintenance of pregnancy in ruminants PMID: 16574787
  22. effect of IFN-tau on the proliferation and distribution of peripheral blood lymphocyte subsets during one-way mixed lymphocyte reaction (MLR) in cows and heifers PMID: 16870264
  23. abnormal gene expression of DNMT, INFT, and MHC1 was noted in the majority of cloned embryos, indicating inefficient nuclear reprogramming and retarded embryo development. PMID: 18199878
  24. DLX3 has a central role in controlling IFNT gene expression by associating with ETS2 on the IFNT promoter. PMID: 18322277
  25. Endogenous IFNT transcription in MDBK cells (which do not normally express IFNT) can be induced through CDX2 overexpression and high H3K18 acetylation. PMID: 19211809

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Subcellular Location
Secreted. Note=Secreted into the uterine lumen.
Protein Families
Alpha/beta interferon family, IFN-alphaII subfamily
Tissue Specificity
Constitutively and exclusively expressed in the mononuclear cells of the extraembryonic trophectoderm.
Database Links

KEGG: bta:317698

STRING: 9913.ENSBTAP00000047689

UniGene: Bt.328

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