Bovine Selenoprotein P(SEPP1) ELISA kit

Code CSB-EL021018BO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name selenoprotein P, plasma, 1
Alternative Names SELENOP ELISA Kit; Selenoprotein P ELISA Kit; SeP ELISA Kit; Selenoprotein P-like protein ELISA Kit
Abbreviation SEPP1
Uniprot No. P49907
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 10 ng/mL-160 ng/mL
Sensitivity 5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of bovine SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 89
Range % 86-102
1:200 Average % 97
Range % 91-105
1:400 Average % 93
Range % 88-100
1:800 Average % 91
Range % 87-96
The recovery of bovine SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 86-99
EDTA plasma (n=4) 93 85-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average  
160 0.105 0.102 0.104  
80 0.196 0.189 0.193  
40 0.321 0.332 0.327  
20 0.622 0.608 0.615  
10 1.098 1.108 1.103  
0 2.193 2.216 2.205  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Constitutes a major selenium pool in the brain and may play an important role in developing and/or modulating the morphology of neurons and/or glial cells.
Subcellular Location Secreted
Protein Families Selenoprotein P family
Tissue Specificity Brain and kidney. Most prominently expressed in the cerebellar cortex, hippocampus and olfactory bulb.
Database Links

KEGG: bta:282066

UniGene: Bt.64610


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