Bovine beta-lactoglobulin (Beta-LG) (allergen Bos d 5) (LGB)ELISA kit

Instructions
Code CSB-EL027168BO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name beta-lactoglobulin (Beta-LG) (allergen Bos d 5) (LGB)ELISA kit
Alternative Names Beta lactoglobulin B ELISA Kit; Beta-lactoglobulin ELISA Kit; Beta-LG ELISA Kit; BLG ELISA Kit; LACB_BOVIN ELISA Kit; LGB ELISA Kit
Abbreviation LGB
Uniprot No. P02754
Species Bos taurus (Bovine)
Sample Types serum, plasma, milk
Detection Range 0.156 μg/mL-10 μg/mL
Sensitivity 0.129 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine LGB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %87-101
1:2Average %92
Range %90-95
1:4Average %89
Range %85-93
1:8Average %95
Range %90-98
Recovery
The recovery of bovine LGB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9693-99
EDTA plasma (n=4)9489-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2Average
100.173 0.168 0.171
50.279 0.273 0.276
2.50.376 0.358 0.367
1.250.482 0.472 0.477
0.6250.631 0.653 0.642
0.3130.948 0.914 0.931
0.1561.166 1.193 1.180
01.884 1.952 1.918
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
Gene References into Functions
  1. the conformational complexity of BLG protein-protein self-association and palmitic acid (PLM) or dodecyl sulfate (SDS) ligand recognition in the monomeric and dimeric state, was investigated. PMID: 27456117
  2. The effect of beta-lactoglobulin (beta-LG) phenotype on the fatty acid composition and antioxidant capacity of milk is variable, which could partly be the result of a beta-LG phenotype x diet interaction. PMID: 26174000
  3. LG plays a key role in enhancing immune responses by promoting cell proliferation through IgM receptor. PMID: 27957499
  4. Interactions of LGB-A and LGB-B with sodium dodecyl sulfate, dodecyltrimethylammonium chloride and lauric acid, 12-carbon ligands possessing differently charged polar groups, were investigated.[LGB-A, LGB-B] PMID: 23784992
  5. Data (including data from molecular dynamic simulations) suggest that -OH groups of 3-hydroxypalmitic acid and 16-hydroxypalmitic acid act as hydrogen-bond donors in a hydrophobic protein cavity of beta-lactoglobulin. [BLG, beta-lactoglobulin] PMID: 24658798
  6. SNPs at the exon2 region of the beta-LG gene affected milk performance traits in Chinese Holstein cattle. PMID: 22097730
  7. Rate constants (k(off)) for betaLg dimer dissociation, were estimated. PMID: 22853908
  8. Because of a more favorable enthalpy, the dimerization equilibrium constant of beta-LG B was larger than that of beta-LG A at room temperature, while the two dimers became similarly stable at 35 degrees C. PMID: 21117642
  9. Three single nucleotide polymorphisms change beta-LG B* gene structure and expression. PMID: 21750913
  10. Kinetic studies of the interaction of beta-lactoglobulin with model membranes: stopped-flow CD and fluorescence studies. PMID: 20822106
  11. multiple binding regions with a specific and different affinity for structurally different classes of hydrophobic drugs and, that bovine beta-lactoglobulin can bind and protect against low pH values various classes of drugs of pharmaceutical relevance. PMID: 19919177
  12. Studies show that the addition of salts to water at pH 3.0 favors the dimerization of beta-lactoglobulin, and the dimer is the dominant species at 1M NaCl, 1M GuHCl, and 25 mM NaClO(4). PMID: 19241465
  13. The stability of beta-lactoglobulin against unfolding is sharply affected by the pH of the medium: both A and B LGB variants are maximally stabilized against urea denaturation at acidic pH and against sodium dodecyl sulfate denaturation at alkaline pH. PMID: 12484782
  14. association between single nucleotide polymorphism in the activator protein-2 binding site of the beta-lactoglobulin gene and its gene product can be explained as the result of differences in protein binding activity PMID: 12836958
  15. In apo-beta-lactoglobulin simulations starting from the protonated crystal structure, a long-lived H-bond interaction between the protonated Glu89 and Ser116 was observed PMID: 14997570
  16. Results suggest that the stability of beta-lactoglobulin arising from the hydrophobic effect is reduced by the C121S mutation so that unfolded or partially unfolded states are more favored. PMID: 15147215
  17. PPIX binds a bLG site affected by the pH of the solution PMID: 16297563
  18. Data describe the Tanford transition, a conformational change of bovine beta-lactoglobulin (betaLG) occurring at around pH 7, using heteronuclear NMR. PMID: 16368109
  19. bovine beta-lactoglobulin (variant A) structural analysis at very low ionic strength PMID: 16540345
  20. Comparison among the various probes shows multiple binding sites for hydrophobes are present in the native beta-lactoglobulin (BLG)and in the partially-and reversibly-modified form of BLG present in solution at neutral pH and subdenaturing temperatures. PMID: 16721656
  21. the interaction and orientation of betaLG in association with various artificial lipid vesicles at neutral and acidic pH have been studied by use of several complementary spectroscopic techniques. PMID: 16819842
  22. Found a C to A transversion at position 215 bp upstream of the translation initiation site (g.-215C>A)that segregated perfectly with differential phenotypic expression in a paternal half-sib family. PMID: 17033029
  23. Beta lactoglobulin provides an example of binding with low specificity but high affinity, which may play an important role in protein-ligand and protein-protein networks. PMID: 17331535
  24. analysis of pH-dependent conformational transitions of bovine beta-lactoglobulin monitored by heteronuclear NMR PMID: 17878316
  25. the contributions of interface area desolvation and water immobilization accounted satisfactorily for the experimental heat capacity PMID: 17910061
  26. Human LIMR, known to act as an endocytic receptor for lipocalin-1, also binds bovine BLG and mediates its cellular uptake. PMID: 17991420
  27. A model reveals that one vitamin D3 molecule binds to the calyx (central internal binding-site) of beta-LG and the other binds to the surface of beta-LG between the alpha-helix and beta-strand I (external binding site). PMID: 18004750
  28. The three-dimensional structure of BLG complexed with Ige Fab fragments provides the first insight into IgE antibody-allergen interactions at the molecular level. PMID: 18097096
  29. In heterozygotes, beta-casein A and beta-lactoglobulin A were found at higher concentrations in milk compared with the variant encoded by the alternative allele whereas kappa-casein A and B variants were found at similar concentrations in AB heterozygotes PMID: 18363977
  30. The extreme case of a completely dehydrated free binding site is realized for the large nonpolar binding cavity in bovine beta-lactoglobulin. PMID: 18427121
  31. bovine beta-lactoglobulin dimers folding PMID: 18465840
  32. No differences were found in milk compositional characteristics among genetic variants of beta-lactoglobulin in 2 breeds of cows; no association between milk composition and beta-lactoglobulin was observed. PMID: 18474134
  33. Noncovalent labeling with thioflavin T and 1-anilino-8-naphthalenesulfonate was used to follow the conformational changes occurring in beta-lactoglobulin during aggregation using time resolved luminescence. PMID: 18590743
  34. the expression of soluble wild type recombinant BGL in Escherichia coli cells at about 109 mg rBLG/g wet weight cells and a comparison between the aggregation of wt BLG and its variant C121S upon intracellular expression. PMID: 18662787
  35. DNA methylation changes in cell line from beta-lactoglobulin gene targeted fetus. PMID: 18789616
  36. detected new polymorphisms in the beta-lactoglobulin (beta-LG) gene with significant effects on beta-LG protein concentration PMID: 19032698

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Subcellular Location Secreted
Protein Families Calycin superfamily, Lipocalin family
Tissue Specificity Synthesized in mammary gland and secreted in milk.
Database Links

KEGG: bta:280838

STRING: 9913.ENSBTAP00000019538

UniGene: Bt.385

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