Canine Matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

Code CSB-E14056c
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Matrix metalloproteinase 2/Gelatinase A,MMP-2
Abbreviation MMP2/Gelatinase A
Uniprot No. F1PMK7
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 4.69 ng/mL-300 ng/mL
Sensitivity 1.17 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %98
Range %94-106
1:2Average %85
Range %80-95
1:4Average %95
Range %92-100
1:8Average %93
Range %86-98
Recovery
The recovery of canine MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-101
EDTA plasma (n=4)9287-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
3002.028 1.952 1.990 1.854
1501.736 1.776 1.756 1.620
751.502 1.452 1.477 1.341
37.51.128 1.139 1.134 0.998
18.750.757 0.785 0.771 0.635
9.380.503 0.524 0.514 0.378
4.690.331 0.329 0.330 0.194
00.137 0.134 0.136  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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