Target Name
Matrix metalloproteinase 2/Gelatinase A,MMP-2
Abbreviation
MMP2/Gelatinase A
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
4.69 ng/mL-300 ng/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
1:1 | Average % | 98 | |
Range % | 94-106 | |
1:2 | Average % | 85 | |
Range % | 80-95 | |
1:4 | Average % | 95 | |
Range % | 92-100 | |
1:8 | Average % | 93 | |
Range % | 86-98 | |
Recovery
The recovery of canine MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
Sample Type | Average % Recovery | Range | |
Serum (n=5) | 96 | 90-101 | |
EDTA plasma (n=4) | 92 | 87-98 | |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
ng/ml | OD1 | OD2 | Average | Corrected | | 300 | 2.028 | 1.952 | 1.990 | 1.854 | | 150 | 1.736 | 1.776 | 1.756 | 1.620 | | 75 | 1.502 | 1.452 | 1.477 | 1.341 | | 37.5 | 1.128 | 1.139 | 1.134 | 0.998 | | 18.75 | 0.757 | 0.785 | 0.771 | 0.635 | | 9.38 | 0.503 | 0.524 | 0.514 | 0.378 | | 4.69 | 0.331 | 0.329 | 0.330 | 0.194 | | 0 | 0.137 | 0.134 | 0.136 | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Dog MMP2/Gelatinase A ELISA Kit was designed for the quantitative measurement of Dog MMP2/Gelatinase A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.69 ng/mL-300 ng/mL and the sensitivity is 1.17 ng/mL .