Fish (Salmon) leptin (LEP) ELISA kit

Code CSB-EL012870FI
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
leptin (LEP)
Uniprot No.
Sample Types
serum, plasma
Detection Range
62.5 pg/mL-1000 pg/mL
31.25 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of fish LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 91  
Range % 86-98  
1:40 Average % 97  
Range % 91-102  
1:80 Average % 84  
Range % 80-87  
1:160 Average % 90  
Range % 83-96  
The recovery of fish LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-96  
EDTA plasma (n=4) 97 93-102  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1000 0.082 0.086 0.084    
500 0.190 0.200 0.195    
250 0.483 0.444 0.464    
125 0.945 0.959 0.952    
62.5 1.672 1.555 1.614    
0 2.591 2.670 2.631    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Fish LEP ELISA Kit was designed for the quantitative measurement of Fish LEP protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 62.5 pg/mL-1000 pg/mL and the sensitivity is 31.25 pg/mL.


Customer Reviews and Q&A

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Have you ever tested tissue sample?

Thanks for your inquiry!We only tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test tissue samplet,we suggest do pre-testing first.Here is the processing way on tissue samples.Pls check for your reference:
Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was removed and assayed immediately. Alternatively, aliquot and store samples at -20°C or -80°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

Is this kit also compatible to test Leptin levels in Zebrafish?

Thanks for your inquiry!We suggest you do a pretest if you want to test zebrafish.

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
d. Please send the manual.
e. Please provide your reference data for fish plasma samples.

Thanks for your inquiry.
This is a competitive kit. There is only one antibody in the kit.
The plate is coated with mouse monoclonal antibody. ( Immunogen is CHO-derived recombinant protein.)
Standard is CHO-derived recombinant protein.
Pls find attached manual.
We tested salmon serum and plasma and some common freshwater fish before and the test value is about
125pg/ml under 1:100 dilution. The final concentration is about 12.5ng/ml.
Pls let me know if you have any further questions. Thank you.
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