Fish growth hormone,GH ELISA Kit

Code CSB-E12121Fh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Fish growth hormone (GH) ELISA Kit was designed for the quantitative measurement of Fish growth hormone (GH) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 312.5 pg/mL-5000 pg/mL and the sensitivity is 312.5 pg/mL.

Target Name growth hormone,GH
Alternative Names gh1 ELISA Kit; Somatotropin-1 ELISA Kit; Growth hormone I ELISA Kit; Somatotropin I ELISA Kit
Abbreviation GH
Uniprot No. Q91222
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 312.5 pg/mL-5000 pg/mL
Sensitivity 312.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 91-98  
1:2 Average % 92  
Range % 88-96  
1:4 Average % 102  
Range % 96-106  
1:8 Average % 96  
Range % 92-101  
Recovery
The recovery of fish GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 92-98  
EDTA plasma (n=4) 94 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
5000 0.271 0.265 0.268    
2500 0.526 0.514 0.520    
1250 0.887 0.863 0.875    
625 1.664 1.684 1.674    
312.5 2.557 2.602 2.580    
Materials provided
  • A 96-well Assay plate --The 96-well plate has been pre-coated with anti-Fish GH antibody.
  • Standard (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
  • HRP-avidin (1 x 6 ml) --Catalyze the TMB to elicit a chromogenic reaction.
  • Biotin-conjugated GH (1 x 6 ml) --Bind to the precoated antibody and act as the detection antigen.
  • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
  • Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances.
  • Substrate A (1x 7 ml) --Mix with Sbustrate B.
  • Substrate B (1x 7 ml) --Mix with Sbustrate A
  • Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Growth hormone plays an important role in growth control and is involved in the regulation of several anabolic processes. Implicated as an osmoregulatory substance important for seawater adaptation.
Subcellular Location Secreted.
Protein Families Somatotropin/prolactin family
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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