Goat Heat Shock Protein 70(HSP-70) ELISA KIT

Code CSB-E13381G
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Goat HSPA2 ELISA Kit was designed for the quantitative measurement of Goat HSPA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.44 ng/mL-1500 ng/mL and the sensitivity is 5.86 ng/mL.

Target Name heat shock 70kDa protein 2
Alternative Names HSPA2 ELISA Kit; HSP70-3 ELISA Kit; Heat shock-related 70 kDa protein 2 ELISA Kit; Heat shock 70 kDa protein 3 ELISA Kit; HSP70.3 ELISA Kit
Abbreviation HSPA2
Uniprot No. Q9TUG3
Species Capra hircus (Goat)
Sample Types serum, plasma, tissue homogenates
Detection Range 23.44 ng/mL-1500 ng/mL
Sensitivity 5.86 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-99
1:2Average %92
Range %85-99
1:4Average %95
Range %92-103
1:8Average %89
Range %82-98
Recovery
The recovery of goat HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-98
EDTA plasma (n=4)9589-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
15002.308 2.463 2.386 2.234
7501.606 1.666 1.636 1.484
3751.078 1.064 1.071 0.919
187.50.732 0.721 0.727 0.575
93.750.428 0.456 0.442 0.290
46.880.313 0.330 0.322 0.170
23.440.219 0.235 0.227 0.075
00.151 0.153 0.152  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells.
Subcellular Location Cytoplasm, cytoskeleton, spindle.
Protein Families Heat shock protein 70 family
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