Goat Serum amyloid A(SAA) ELISA Kit

Code CSB-E13212G
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. A5JST2
Lead Time 3-5 working days
Abbreviation SAA
Target Name Serum amyloid A(SAA)
Species Capra hircus (Goat)
Sample Types serum, plasma, tissue homogenates
Detection Range 100 ng/mL-8000 ng/mL
Sensitivity 125 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 91-101  
1:2 Average % 92  
Range % 87-96  
1:4 Average % 95  
Range % 91-98  
1:8 Average % 96  
Range % 91-103  
Recovery
The recovery of goat SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-105  
EDTA plasma (n=4) 94 89-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
8000 0.121 0.126 0.124    
3000 0.208 0.220 0.214    
1500 0.331 0.354 0.343    
500 0.594 0.587 0.591    
100 1.265 1.249 1.257    
0 2.648 2.544 2.596    
References
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