Goat gonadotropin-releasing hormone,GnRH ELISA Kit

Code CSB-E13276G
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
gonadotropin-releasing hormone,GnRH
Abbreviation
GnRH
Uniprot No.
Species
Capra hircus (Goat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
2.5 pg/mL-500 pg/mL
Sensitivity
1.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-99
1:2Average %95
Range %88-102
1:4Average %94
Range %89-99
1:8Average %95
Range %89-103
Recovery
The recovery of goat GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9588-102
EDTA plasma (n=4)9791-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
02.557 2.458 2.508
2.51.495 1.481 1.488
101.053 1.045 1.049
400.650 0.636 0.643
1250.408 0.392 0.400
5000.222 0.214 0.218
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Goat GnRH ELISA Kit was designed for the quantitative measurement of Goat GnRH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 pg/mL-500 pg/mL and the sensitivity is 1.25 pg/mL .

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