Goat gonadotropin-releasing hormone,GnRH ELISA Kit

Instructions
Code CSB-E13276G
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name gonadotropin-releasing hormone,GnRH
Abbreviation GnRH
Uniprot No. D3YJ02
Species Capra hircus (Goat)
Sample Types serum, plasma, tissue homogenates
Detection Range 2.5 pg/mL-500 pg/mL
Sensitivity 1.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-99
1:2Average %95
Range %88-102
1:4Average %94
Range %89-99
1:8Average %95
Range %89-103
Recovery
The recovery of goat GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9588-102
EDTA plasma (n=4)9791-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
02.557 2.458 2.508
2.51.495 1.481 1.488
101.053 1.045 1.049
400.650 0.636 0.643
1250.408 0.392 0.400
5000.222 0.214 0.218
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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