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Guinea pig interleukin 13,IL-13 ELISA Kit

Instructions
Code CSB-E12916Gp
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 13,IL-13
Abbreviation IL13
Uniprot No. H0W834
Species Cavia porcellus (Guinea pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.5 pg/mL-300 pg/mL
Sensitivity 3.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Guinea pig IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 88
Range % 83-92
1:2 Average % 95
Range % 85-105
1:4 Average % 93
Range % 86-102
1:8 Average % 97
Range % 92-110
Recovery
The recovery of Guinea pig IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 91-103
EDTA plasma (n=4) 93 86-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average
300 2.244 2.267 2.256
150 1.173 1.161 1.167
75 0.628 0.650 0.639
37.5 0.359 0.343 0.351
15 0.152 0.151 0.152
7.5 0.083 0.079 0.081
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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