Human α2 macroglobulin,α2 MG ELISA Kit

Code CSB-E08959h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01023
Lead Time 3-5 working days
Abbreviation A2M
Target Name alpha-2-macroglobulin
Alias CPAMD5, DKFZp779B086, FWP007, S863-7,
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.25 μg/mL-20 μg/mL
Sensitivity 1.25 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits.
HGNC 7
RGD 2004
MGI 2449119
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human α2 MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %87
Range %82-93
1:200Average %94
Range %89-99
1:400Average %95
Range %89-99
1:800Average %92
Range %87-96
Recovery
The recovery of human α2 MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9285-98
EDTA plasma (n=4)9692-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2Average
200.185 0.187 0.186
100.360 0.365 0.363
50.635 0.642 0.639
2.51.163 1.172 1.168
1.251.995 2.013 2.004
02.849 2.924 2.887
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Function Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Subcellular Location Secreted
Protein Families Protease inhibitor I39 (alpha-2-macroglobulin) family
Tissue Specificity Secreted in plasma.
Database Links

HGNC: 7

OMIM: 103950

KEGG: hsa:2

STRING: 9606.ENSP00000323929

UniGene: Hs.212838

Pathway Complement and coagulation cascades

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