Human 14-3-3 protein gamma(YWHAG) ELISA kit

Instructions
Code CSB-EL026288HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
Alternative Names 14 3 3 gamma ELISA Kit; 14 3 3 protein gamma ELISA Kit; 14 3 3 protein gamma subtype ELISA Kit; 14 3 3gamma ELISA Kit; 14-3-3 protein gamma ELISA Kit; 1433G_HUMAN ELISA Kit; 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide ELISA Kit; KCIP 1 ELISA Kit; KCIP-1 ELISA Kit; KCIP1 ELISA Kit; N-terminally processed ELISA Kit; Protein kinase C inhibitor protein 1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide ELISA Kit; Ywhag ELISA Kit
Abbreviation YWHAG
Uniprot No. P61981
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 86-96  
1:2 Average % 87  
Range % 83-93  
1:4 Average % 93  
Range % 88-98  
1:8 Average % 94  
Range % 87-97  
Recovery
The recovery of human YWHAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 108 102-112  
EDTA plasma (n=4) 97 92-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.623 2.745 2.684 2.578  
20 2.178 2.272 2.225 2.119  
10 1.531 1.633 1.582 1.476  
5 0.938 0.917 0.928 0.822  
2.5 0.522 0.504 0.513 0.407  
1.25 0.337 0.342 0.340 0.234  
0.625 0.233 0.242 0.238 0.132  
0 0.105 0.106 0.106    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Gene References into Functions
  1. 14-3-3gamma protein directly interacts with the kinase domain of CaMKK2 and the region containing the inhibitory phosphorylation site Thr(145) within the N-terminal extension. CaMKK isoforms differ in their 14-3-3-mediated regulations and the interaction between 14-3-3 protein and the N-terminal 14-3-3-binding motif of CaMKK2 might be stabilized by small-molecule compounds. PMID: 29649512
  2. Findings indicate the roles and molecular mechanisms of 14-3-3GAMMA protein (14-3-3gamma) in epithelial-mesenchymal transition (EMT), migration, and neoplasm invasion. PMID: 29848704
  3. Study demonstrated a mechanism by which 14-3-3gamma restricts centrosome duplication to once per cell cycle, by inhibiting cdc25C and cdk1 activation resulting in decrease phosphorylation of T199 in NPM1. Also, loss of 14-3-3gamma causes centrosome over-duplication, centrosome clustering and tumor formation in mice. PMID: 27253419
  4. Study identified 14-3-3gamma as a binding partner for segment a of ANO1 and enhanced the surface expression of ANO1. In addition, its gene silencing of 14-3-3gamma inhibited migration and invasion of these glioblastoma cell lines. PMID: 27212225
  5. These results suggest that a major role of 14-3-3gamma in desmosome assembly is to transport PG to the cell border leading to the initiation of desmosome formation. PMID: 29253567
  6. hypermethylation of the 14-3-3 gene promoter accounts for the decreased 14-3-3 gamma in uterine leiomyomas and that such a low level of expression may be involved in the pathogenesis of leiomyomas PMID: 27100507
  7. MiR-217 directly targeted 3'UTR of YWHAG and suppressed the expression of YWHAG. PMID: 28126486
  8. Data indicate angiopoietin-like 4 (ANGPTL4) as a key player that coordinates an increase in cellular energy flux crucial for EMT via an ANGPTL4/14-3-3gamma signaling axis. PMID: 28745316
  9. 14-3-3gamma regulates the differentiation ability of CPNE1 through the binding with C2A domain of CPNE1 in HiB5 cells. PMID: 28412242
  10. YWHAG de novo mutations cause early onset epilepsy, including epileptic encephalopathies and intellectual disability. PMID: 28777935
  11. Study found that the overexpression of 14-3-3gamma in utero in the developing mouse cortex results in delays in pyramidal neuron migration. PMID: 27288018
  12. The present study revealed the tumor suppressive role of miR-509-5p in non-small cell lung cancer (NSCLC) by targeting YWHAG, suggesting that miR-509-5p/YWHAG axis might be considered as a novel and potential target for clinical diagnosis and therapeutics of NSCLC. PMID: 27894843
  13. 14-3-3 protein expression was quantitatively analyzed in cerebrospinal fluid of 231 sporadic Creutzfeldt-Jakob disease and 2035 control patients. PMID: 25947081
  14. Data suggest that miR-181b-3p functions as a metastasis activator by promoting Snail-induced epithelial-mesenchymal transition in breast cancer cells by directly targeting YWHAG. PMID: 27102539
  15. Loss of p53 function may result in upregulation of 14-3-3gamma in lung cancers PMID: 25384678
  16. Changes for CRMP2, TCP1epsilon, TPM2 and 14-3-3gamma were confirmed in experimental tumors and in a series of 28 human SI-NETs. PMID: 25224486
  17. These results showed that the cell surface expression of TRPM4 channels is mediated by 14-3-3gamma binding. PMID: 25047048
  18. Proteomics analysis show that Ser40 of TH protein does not significantly contribute to the binding of 14-3-3gamma, and rather has reduced accessibility in the TH:14-3-3gamma complex. PMID: 24947669
  19. physiological changes in phosphate anions concentration can modulate affinity and specificity of interaction of 14-3-3 with its multiple targets and therefore the actual phosphointeractome of 14-3-3. PMID: 23977325
  20. High 14-3-3gamma expression was seen in 59.5% of non-small cell lung cancers. PMID: 24870749
  21. miR-141/YWHAG and miR-520e/RAB11A are two potential miRNA/protein target pairs associated with severe obesity. PMID: 22537031
  22. The normalisation capability of 14-3-3 gamma was superior to traditional LC in quantifying Western blot signals of the platelet AD-biomarker Monoamine Oxidase B of patient versus controls. PMID: 24284060
  23. 14-3-3gamma was induced in B cells by T-dependent and T-independent primary CSR-inducing stimuli in vitro. PMID: 23851690
  24. The region surrounding pSer19 of Tyrosine hydroxylase adopts an extended conformation in the 14-3-3gamma-bound state, whereas adopts a bent conformation when free in solution. PMID: 24055376
  25. Mitotic Plk1 activity is regulated not only by Plk1-Thr210 phosphorylation, but also by Plk1 binding to 14-3-3gamma following Plk1-Ser99 phosphorylation downstream of the PI3K-Akt signalling pathway. PMID: 23695676
  26. high 14-3-3gamma expression is associated with poor survival in NPC patients. Thus, this study has identified that the 14-3-3gamma involves in the carcinogenesis of NPC. PMID: 23500129
  27. Increased expression of 14-3-3gamma in breast cancer is associated significantly with tumor progression and poor prognosis. PMID: 22658894
  28. The peripheral binding of 14-3-3gamma to membranes involves isoform-specific histidine residues. PMID: 23189152
  29. Using individual amino acid substitutions within the 14-3-3gamma VRII, we identified two residues required for and two contributing to the gamma-specific phenotypes. PMID: 23115241
  30. 14-3-3gamma protein binds strongly to long DNA targets and shows very strong preference for supercoiled DNA. PMID: 22856523
  31. hypoxia can activate p53 through inactivation of MDMX by the ATR-Chk1-MDMX-14-3-3gamma pathway. PMID: 22556425
  32. description of a protein complex that mediates carrier formation and contains budding and fission molecules, as well as other molecules, such as the adaptor protein 14-3-3gamma PMID: 22366688
  33. Increased expression of 14-3-3 gamma in lung cancer coincides with loss of functional p53. PMID: 21867493
  34. results support that membrane binding involves the non-conserved, convex area of 14-3-3gamma, and that Trp residues do not intercalate in the bilayer. PMID: 21420405
  35. a new role for 14-3-3gamma in protecting p21 from MDMX-mediated proteasomal turnover, which may partially account for DNA damage-induced elevation of p21 levels independent of p53. PMID: 21148311
  36. data do not exclude the possibility that YWHAG loss of function is also sufficient to cause neurological phenotypes PMID: 21109226
  37. Elevated expression of 14-3-3gamma in human hepatocellular carcinoma predicts extrahepatic metastasis and worse survival PMID: 20870266
  38. 14-3-3 protein gamma forms a complex with Checkpoint kinase 1 phosphorylated at Ser296, but not at ATR sites (Ser317 and Ser345). PMID: 20639859
  39. Abundant level of the phosphorylated FOXO1, its impaired nucleocytoplasmic shuttling, and the lowered expression of 14-3-3 protein in leiomyoma induces a shift in the cellular machinery toward a prosurvival execution program. PMID: 19772960
  40. 14-3-3 eta, beta, gamma and sigma isoforms were negatively expressed in meningioma. PMID: 20388496
  41. Zebrafish gene knockdowns imply roles for human YWHAG in infantile spasms and cardiomegaly. PMID: 20146355
  42. Aberrant expression of signaling-related proteins 14-3-3 gamma and RACK1 in fetal Down syndrome brain (trisomy 21). PMID: 11824616
  43. 14-3-3 suppresses importin alpha/beta-dependent nuclear localization of Thr157-phosphorylated p27, suggesting implications for cell cycle disorder in Akt-activated cancer cells. PMID: 15057270
  44. multiple interactions of AICD with FE65 and 14-3-3gamma modulate FE65-dependent gene transactivation PMID: 16223726
  45. Interaction of human 14-3-3gamma with the small heat shock protein Hsp20 was analyzed by means of size-exclusion chromatography and chemical crosslinking. PMID: 17109079
  46. Both single marker and haplotype analyses were negative for the Ywhag gene association with smoking initiation and nicotine dependence. PMID: 17284169
  47. our data indicate that 14-3-3gamma may contribute to tumorigenesis by promoting genomic instability. PMID: 17394238
  48. Detection of only 2 (14-3-3 eta and gamma) out of 7 different isoforms in synovial fluid suggests they are specific to the site of joint inflammation PMID: 17611984
  49. Report reduced expression of 14-3-3 gamma in uterine leiomyoma as identified by proteomics. PMID: 18054924
  50. human 14-3-3gamma binds to the ERK1/2 molecular scaffold KSR1, which is mediated by the C-terminal stretch of 14-3-3gamma PMID: 18426801

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Subcellular Location Cytoplasm
Protein Families 14-3-3 family
Tissue Specificity Highly expressed in brain, skeletal muscle, and heart.
Database Links

HGNC: 12852

OMIM: 605356

KEGG: hsa:7532

STRING: 9606.ENSP00000306330

UniGene: Hs.744840

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