YWHAG

YWHAG encodes the 14-3-3 gamma protein, also commonly known as 14-3-3G and YWHAG. This protein functions as an adapter protein involved in signal transduction and cell cycle regulation. It interacts with a wide range of target proteins through phosphorylated serine and threonine residues, modulating their activity and subcellular localization. The 14-3-3 gamma is involved in the MAPK and PI3K/Akt signaling pathways.
Alterations in YWHAG are associated with various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Given its crucial role in multiple pathways, it has emerged as a potential drug target. Some research is exploring small - molecule inhibitors, but as of now, no drugs targeting 14 - 3 - 3 gamma have reached the market.

Validated Data

Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, K562 whole cell lysate
All lanes: YWHAG antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa

IHC image of CSB-RA824302A0HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

YWHAG Antibody (CSB-PA026288LA01HU)

Validated Data

Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA026288LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA026288LA01HU at dilution of 1:100

Western Blot
Positive WB detected in: Mouse brain tissue, A431 whole cell lysate, Human placenta tissue
All lanes: YWHAG antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa

Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate, Rat brain tissue
All lanes: YWHAG antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa

Immunoprecipitating YWHAG in NIH/3T3 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA026288LA01HU in NIH/3T3 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA026288LA01HU (5μg) + NIH/3T3 whole cell lysate (500μg)
Lane 3: NIH/3T3 whole cell lysate (20μg)

Western Blot
Positive WB detected in: Mouse Brain tissue lysate,Rat Brain tissue lysate,NIH/3T3 whole cell lysate, Rat Lung tissue lysate,Mouse Lung tissue lysate,Rat Liver tissue lysate,Rat Kidney tissue lysate,Mouse Kidney tissue lysate
All lanes: YWHAG antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28.3 kDa
Observed band size: 28kDa

Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA026288LA01HU at dilution of 1:100

Human 14-3-3 protein gamma(YWHAG) ELISA kit (CSB-EL026288HU)

Validated Data

Code: CSB-EL026288HU

Size: 96T,5×96T,10×96T

Sensitivity: 0.156 ng/mL

Detection Range: 0.625 ng/mL-40 ng/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following YWHAG reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

YWHAG Antibodies

YWHAG Proteins

YWHAG ELISA Kits

YWHAG Antibodies

YWHAG Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA026288GA01HU	YWHAG Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA948368	YWHAG Antibody	Human,Mouse,Rat	ELISA,WB,IHC,IF
CSB-PA060090	YWHAG Antibody	Human,Mouse,Rat	WB, IHC, IF, ELISA
CSB-PA066436	YWHAG Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA589658	YWHAG Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA026288LA01HU	YWHAG Antibody	Human, Mouse, Rat	ELISA, WB, IHC, IP
CSB-PA026288LB01HU	YWHAG Antibody, HRP conjugated	Human	ELISA
CSB-PA026288LD01HU	YWHAG Antibody, Biotin conjugated	Human	ELISA
CSB-RA824302A0HU	YWHAG Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC

YWHAG Proteins

YWHAG Proteins for Ovis aries (Sheep)

Code	Product Name	Source
CSB-YP026288SH CSB-EP026288SH CSB-BP026288SH CSB-MP026288SH CSB-EP026288SH-B	Recombinant Sheep 14-3-3 protein gamma (YWHAG)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

YWHAG Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP026288BO CSB-EP026288BO CSB-BP026288BO CSB-MP026288BO CSB-EP026288BO-B	Recombinant Bovine 14-3-3 protein gamma (YWHAG)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

YWHAG Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP026288MO CSB-EP026288MO CSB-BP026288MO CSB-MP026288MO CSB-EP026288MO-B	Recombinant Mouse 14-3-3 protein gamma (Ywhag)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

YWHAG Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP026288HU CSB-EP026288HU CSB-BP026288HU CSB-MP026288HU CSB-EP026288HU-B	Recombinant Human 14-3-3 protein gamma (YWHAG)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

YWHAG Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP026288RA CSB-EP026288RA CSB-BP026288RA CSB-MP026288RA CSB-EP026288RA-B	Recombinant Rat 14-3-3 protein gamma (Ywhag)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

YWHAG Proteins for Gallus gallus (Chicken)

Code	Product Name	Source
CSB-YP704881CH CSB-EP704881CH CSB-BP704881CH CSB-MP704881CH CSB-EP704881CH-B	Recombinant Chicken 14-3-3 protein gamma (YWHAG)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

YWHAG Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)

Code	Product Name	Source
CSB-YP712963PYX CSB-EP712963PYX CSB-BP712963PYX CSB-MP712963PYX CSB-EP712963PYX-B	Recombinant Pongo abelii 14-3-3 protein gamma (YWHAG)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

YWHAG ELISA Kit

YWHAG ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-EL026288HU	Human 14-3-3 protein gamma(YWHAG) ELISA kit	serum, plasma, tissue homogenates	0.156 ng/mL

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YWHAG

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