Human 5-hydroxyeicosatetraenoic acid(5-HETE)ELISA Kit

Code CSB-E17006h
Size 96T How to order?
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name 5-hydroxyeicosatetraenoic acid(5-HETE)
Abbreviation 5-HETE
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Biochemicals
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HETE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 89  
Range % 84-94  
1:200 Average % 92  
Range % 87-99  
1:400 Average % 93  
Range % 85-97  
1:800 Average % 97  
Range % 90-104  
Recovery
The recovery of human 5-HETE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-97  
EDTA plasma (n=4) 91 84-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.739 2.652 2.696 2.586  
1000 2.258 2.176 2.217 2.107  
500 1.513 1.504 1.509 1.399  
250 0.805 0.742 0.774 0.664  
125 0.434 0.413 0.424 0.314  
62.5 0.275 0.261 0.268 0.158  
31.25 0.182 0.179 0.181 0.071  
0 0.112 0.107 0.110    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2022 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1