Human ADAM DEC1(ADAMDEC1) ELISA kit

Code CSB-EL001297HU
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human ADAMDEC1 ELISA Kit was designed for the quantitative measurement of Human ADAMDEC1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

Target Name ADAM-like, decysin 1
Alternative Names A disintegrin and metalloproteinase domain like protein decysin 1 ELISA Kit; A disintegrin and metalloproteinase domain-like protein decysin-1 ELISA Kit; ADAM DEC1 ELISA Kit; ADAM like decysin 1 ELISA Kit; ADAM like protein decysin 1 ELISA Kit; ADAM-like protein decysin-1 ELISA Kit; ADAMDEC 1 ELISA Kit; Adamdec1 ELISA Kit; ADEC1_HUMAN ELISA Kit; Decysin ELISA Kit; Disintegrin protease ELISA Kit; M12.219 ELISA Kit
Abbreviation ADAMDEC1
Uniprot No. O15204
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMDEC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 87-96
1:2 Average % 96
Range % 90-101
1:4 Average % 94
Range % 89-99
1:8 Average % 97
Range % 92-103
Recovery
The recovery of human ADAMDEC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 99-108
EDTA plasma (n=4) 94 89-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 2.224 2.364 2.294 2.141
2000 1.732 1.667 1.700 1.547
1000 1.127 1.071 1.099 0.946
500 0.702 0.713 0.708 0.555
250 0.431 0.442 0.437 0.284
125 0.314 0.321 0.318 0.165
62.5 0.246 0.252 0.249 0.096
0 0.152 0.154 0.153  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
May play an important role in the control of the immune response and during pregnancy.
Gene References into Functions
  1. Data suggest that activated platelets release ADAMDEC1, which hydrolyzes pro-EGF (epidermal growth factor) to soluble, active HMW-EGF; proteolytic cleavage of pro-EGF first occurs at the C-terminal arginyl residue of the EGF domain; proteolysis is the regulated, rate-limiting step in generating soluble EGF from activated platelets. PMID: 28455445
  2. We hypothesize that these unique features of ADAMDEC1 may have evolved to escape from inhibition by endogenous metalloprotease inhibitors PMID: 25564618
  3. ADAMDEC1 functions as an active metalloprotease. PMID: 23754285
  4. molecular evolution by duplication in a metalloprotease gene cluster on chromosome 8p12 PMID: 12037602
  5. The ADAMDEC 1 haplotype may indicate an underlying mechanism for high FVIII levels in venous thromboembolism. PMID: 18449420
  6. Gene expression profiling performed on tissues obtained from pulmonary sarcoidosis patients identified ADAMDEC1 as a potential pathogenic mediator of lung damage and/or remodeling and may serve as a marker for this disease. PMID: 19218196

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Subcellular Location Secreted.
Tissue Specificity Expressed highly in the small intestine and appendix, moderately in lymph node, mucosal lining of the colon, thymus, spleen and very weakly in the bone marrow. Predominantly expressed in dendritic cells (DC) of the germinal center. Weakly expressed in mon
Database Links

HGNC: 16299

OMIM: 606393

KEGG: hsa:27299

STRING: 9606.ENSP00000256412

UniGene: Hs.521459

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