Human ATP synthase subunit beta, mitochondrial(ATP5B) ELISA kit

Code CSB-EL002350HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P06576
Lead Time 5-7 working days
Protein Biological Process 2 Adenosine synthesis and metabolism
Abbreviation ATP5B
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Alias ATPMB, ATPSB, MGC5231, mitochondrial ATP synthase beta subunit|mitochondrial ATP synthetase, beta subunit
Species Homo sapiens (Human)
Protein Biological Process 3 ATP synthesis
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
HGNC 830
RGD 621368
MGI 107801
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ATP5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-89  
1:2 Average % 96  
Range % 93-99  
1:4 Average % 87  
Range % 84-90  
1:8 Average % 87  
Range % 83-91  
Recovery
The recovery of human ATP5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-96  
EDTA plasma (n=4) 96 93-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1600 2.308 2.356 2.332 2.191  
800 1.597 1.558 1.578 1.437  
400 0.919 0.938 0.929 0.788  
200 0.549 0.587 0.568 0.427  
100 0.405 0.434 0.420 0.279  
50 0.293 0.272 0.283 0.142  
25 0.201 0.205 0.203 0.062  
0 0.141 0.140 0.141    
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Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Subcellular Location Mitochondrion, Mitochondrion inner membrane
Protein Families ATPase alpha/beta chains family
Database Links

HGNC: 830

OMIM: 102910

KEGG: hsa:506

STRING: 9606.ENSP00000262030

UniGene: Hs.406510

Pathway Oxidative Phosphorylation

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