Human ATP synthase subunit beta, mitochondrial(ATP5B) ELISA kit

Code CSB-EL002350HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Alternative Names ATP 5B ELISA Kit; ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide ELISA Kit; ATP synthase subunit beta mitochondrial ELISA Kit; ATP synthase subunit beta; mitochondrial ELISA Kit; atp5b ELISA Kit; ATPB ELISA Kit; ATPB_HUMAN ELISA Kit; ATPMB ELISA Kit; ATPSB ELISA Kit; Epididymis secretory protein Li 271 ELISA Kit; HEL-S-271 ELISA Kit; Mitochondrial ATP synthase beta subunit ELISA Kit; Mitochondrial ATP Synthase Subunit Beta ELISA Kit; Mitochondrial ATP synthetase beta subunit ELISA Kit
Abbreviation ATP5B
Uniprot No. P06576
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human ATP5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-89  
1:2 Average % 96  
Range % 93-99  
1:4 Average % 87  
Range % 84-90  
1:8 Average % 87  
Range % 83-91  
The recovery of human ATP5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-96  
EDTA plasma (n=4) 96 93-99  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1600 2.308 2.356 2.332 2.191  
800 1.597 1.558 1.578 1.437  
400 0.919 0.938 0.929 0.788  
200 0.549 0.587 0.568 0.427  
100 0.405 0.434 0.420 0.279  
50 0.293 0.272 0.283 0.142  
25 0.201 0.205 0.203 0.062  
0 0.141 0.140 0.141    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Gene References into Functions
  1. The results revealed that ATP5B expression is associated with the process of keratinocyte differentiation which may be related to intracellular ATP synthesis. PMID: 28209970
  2. findings show the T163S mutation affects the catalytic activity with a decrease in Ca2+-dependent and an increase in Mg2+-dependent ATP hydrolysis and desensitizes the permeability transition pore to Ca2+, resulting in increased resistance to Ca2+-dependent mitochondrial depolarization and to cell death PMID: 28507163
  3. experiments implicate circulating NEFA in obesity in suppressing muscle protein metabolism, and establish impaired beta-F1-ATPase translation as an important consequence of obesity PMID: 27532680
  4. ATP5B, as a binding partner of a metastasis-related short peptide (B04) on prostate cancer cells, is involved in promoting prostate cancer metastasis. PMID: 28259978
  5. Results show that the relationship between ATPsyn-beta and insulin secretion deficiency suggests that ATPsyn-beta potentially could serve as a marker for type 2 diabetes mellitus disease risk in women with polycystic ovary syndrome. PMID: 28397049
  6. These results suggested that increasing levels of ATP5B and ETFB were associated with worsening renal injury. PMID: 27840937
  7. In this instance, the ATP5B/CALR/HSP90B1/HSPB1/HSPD1-signaling network was revealed as the predominant target which was associated with the majority of the observed protein-protein interactions. As a result, the identified targets may be useful in explaining the anticancer mechanisms of ursolic acid and as potential targets for colorectal cancer therapy. PMID: 28347227
  8. High mRNA levels of ATP5B are associated with glioblastoma. PMID: 26526033
  9. Hypermethylation of ATPsyn-beta gene promoter is associated with a down-regulated mRNA expression and chemoresistance in AML patients. PMID: 26835708
  10. PKA phosphorylates the ATPase inhibitory factor 1 and inactivates its capacity to bind and inhibit the mitochondrial H(+)-ATP synthase. PMID: 26387949
  11. Our study suggested that positive beta2M expression or loss of ATP5B expression in tumor tissues is closely related to the metastasis, invasion, and poor-prognosis of gallbladder cancer. PMID: 25311765
  12. Mitochondrial ATPsyn-beta expression and ATP synthase activity in relapsed/refractory acute myeloid leukemia patients were downregulated. This downregulated expression exhibited a positive correlation with the response to adriamycin of primary cells. PMID: 24391795
  13. ATP5B gene expressions were detected significantly higher in colorectal cancer samples. PMID: 24583174
  14. H2O2 may induce melanogenesis via the upregulation of PAH and activation of cAMP/p-CREB/MITF signaling by increasing intracellular cAMP levels through the induction of ATP5B. PMID: 23523934
  15. Identification of ATP synthase as a lipid peroxide protein adduct in pancreatic islets from humans with and without type 2 diabetes mellitus. PMID: 23463654
  16. miR-127-5p targets the 3'UTR of beta-F1-ATPase mRNA (beta-mRNA) significantly reducing its translational efficiency. PMID: 22433606
  17. Ectopic ATP synthase could be an accessible molecular target for inhibiting HIV-1 proliferation in vivo. PMID: 22753871
  18. Immunohistochemical analysis on a malignant mesothelioma tissue microarray showed cytoplasmic staining in 28 of 33 samples for vimentin and strong cytoplasmic staining in14 and weak in 16 samples for ss-F1-ATPase. PMID: 22022619
  19. Disturbed flow and hypercholesterolemia synergistically promote gamma/delta T-lymphocyte activation by the membrane translocation of ATPSbeta in endothelial cells. PMID: 21193741
  20. Results suggested that the ectopic expression of ATP synthase is a consequence of translocation from the mitochondria. PMID: 20705594
  21. Molecular and functional studies indicate that the interaction of G3BP1 with beta-F1 mRNA inhibits its translation at the initiation level, supporting a role for G3BP1 in the glycolytic switch that occurs in cancer. PMID: 20663914
  22. ATP synthase beta is phosphorylated at multiple sites and shows abnormal phosphorylation at specific sites in insulin-resistant muscle PMID: 20012595
  23. Findings of the present study support the hypothesis that down-regulation of the bioenergetic activity of mitochondria in human tumours is exerted by translation silencing of beta-F1-ATPase mRNA. PMID: 20028336
  24. Co-immunoprecipitation experiments in the presence of alpha-methyl mannose verified the binding of Escherichia coli FimH to ATP synthase beta-subunit of human brain microvascular endothelial cells. PMID: 20067530
  25. Ectopic beta-chain of ATP synthase is an apolipoprotein A-I receptor in hepatic HDL endocytosis PMID: 12511957
  26. Membrane-bound ATP synthase functions as a receptor for CF6 and may have a previously unsuspected role in the genesis of hypertension by modulating the concentration of intracellular hydrogen. PMID: 16230521
  27. adenosine/uridine (AU)-rich element-binding proteins TIA-1 (T-cell intracellular antigen-1), TIAR (TIA-1-related protein), and HuR (Hu antigen R) interact with the beta-F1-ATPase mRNA through an AU-rich sequence located to the 3'-UTR. PMID: 16890199
  28. This short review summarizes demonstrations of ATP5B (complex 5 of oxidative phosphorylation) subunit that show its movement under stereochemical alterations known to be induced during the binding of ADP and synthesis of ATP. PMID: 16927672
  29. cholesterol exposure increased the level of ATPS-beta, along with Cav-1 and cholesterol in caveolae. the ectopic localization of ATPS-beta may participate in the energy balance of cells in response to the change in intracellular cholesterol levels. PMID: 16996794
  30. conclude that ATP synthase beta-subunit may have an important role in the glucolipotoxicity of islet cells PMID: 18284036
  31. Low levels of beta-F1-ATPase are sensitive to combined platinum and 2-deoxy-D-glucose treatment in ovarian carcinoma. PMID: 19567816

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Subcellular Location Mitochondrion, Mitochondrion inner membrane
Protein Families ATPase alpha/beta chains family
Database Links

HGNC: 830

OMIM: 102910

KEGG: hsa:506

STRING: 9606.ENSP00000262030

UniGene: Hs.406510


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