Product Details

Uniprot No.

P06576

Target Names

ATP5B

Alternative Names

ATP synthase subunit beta, mitochondrial (EC 3.6.3.14), ATP5B, ATPMB ATPSB

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human ATPB

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5F10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ATP5F1B, the beta subunit of mitochondrial ATP synthase, serves as the catalytic core of the enzyme complex responsible for oxidative phosphorylation—the fundamental process by which cells generate ATP. This protein plays a central role in cellular energy metabolism, making it an essential marker for studies investigating mitochondrial function, metabolic reprogramming in cancer, and bioenergetic pathways across diverse tissue types.

This recombinant monoclonal antibody, generated from clone 5F10, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments. The rabbit IgG format provides excellent signal-to-noise characteristics for detection applications.

Validation data demonstrates robust performance across multiple platforms. In western blot applications, this antibody detects a clean band at the expected 57 kDa molecular weight across a range of human cell lines including 293T, HT29, HepG2, Jurkat, and 293 cells, with effective dilutions ranging from 1:500 to 1:5000. Cross-species reactivity extends to rodent samples, with confirmed detection in both rat and mouse heart tissue, providing flexibility for researchers working with animal models. Immunohistochemistry validation in paraffin-embedded human heart and liver tissue at 1:100 dilution shows specific staining patterns consistent with the mitochondria-rich nature of these metabolically active organs.

Whether investigating tumor metabolism, characterizing mitochondrial dysfunction, or exploring signal transduction pathways linked to cellular energetics, this antibody provides a reliable tool for detecting ATP5F1B across your experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Gene References into Functions

The results revealed that ATP5B expression is associated with the process of keratinocyte differentiation which may be related to intracellular ATP synthesis. PMID: 28209970
findings show the T163S mutation affects the catalytic activity with a decrease in Ca2+-dependent and an increase in Mg2+-dependent ATP hydrolysis and desensitizes the permeability transition pore to Ca2+, resulting in increased resistance to Ca2+-dependent mitochondrial depolarization and to cell death PMID: 28507163
experiments implicate circulating NEFA in obesity in suppressing muscle protein metabolism, and establish impaired beta-F1-ATPase translation as an important consequence of obesity PMID: 27532680
ATP5B, as a binding partner of a metastasis-related short peptide (B04) on prostate cancer cells, is involved in promoting prostate cancer metastasis. PMID: 28259978
Results show that the relationship between ATPsyn-beta and insulin secretion deficiency suggests that ATPsyn-beta potentially could serve as a marker for type 2 diabetes mellitus disease risk in women with polycystic ovary syndrome. PMID: 28397049
These results suggested that increasing levels of ATP5B and ETFB were associated with worsening renal injury. PMID: 27840937
In this instance, the ATP5B/CALR/HSP90B1/HSPB1/HSPD1-signaling network was revealed as the predominant target which was associated with the majority of the observed protein-protein interactions. As a result, the identified targets may be useful in explaining the anticancer mechanisms of ursolic acid and as potential targets for colorectal cancer therapy. PMID: 28347227
High mRNA levels of ATP5B are associated with glioblastoma. PMID: 26526033
Hypermethylation of ATPsyn-beta gene promoter is associated with a down-regulated mRNA expression and chemoresistance in AML patients. PMID: 26835708
PKA phosphorylates the ATPase inhibitory factor 1 and inactivates its capacity to bind and inhibit the mitochondrial H(+)-ATP synthase. PMID: 26387949
Our study suggested that positive beta2M expression or loss of ATP5B expression in tumor tissues is closely related to the metastasis, invasion, and poor-prognosis of gallbladder cancer. PMID: 25311765
Mitochondrial ATPsyn-beta expression and ATP synthase activity in relapsed/refractory acute myeloid leukemia patients were downregulated. This downregulated expression exhibited a positive correlation with the response to adriamycin of primary cells. PMID: 24391795
ATP5B gene expressions were detected significantly higher in colorectal cancer samples. PMID: 24583174
H2O2 may induce melanogenesis via the upregulation of PAH and activation of cAMP/p-CREB/MITF signaling by increasing intracellular cAMP levels through the induction of ATP5B. PMID: 23523934
Identification of ATP synthase as a lipid peroxide protein adduct in pancreatic islets from humans with and without type 2 diabetes mellitus. PMID: 23463654
miR-127-5p targets the 3'UTR of beta-F1-ATPase mRNA (beta-mRNA) significantly reducing its translational efficiency. PMID: 22433606
Ectopic ATP synthase could be an accessible molecular target for inhibiting HIV-1 proliferation in vivo. PMID: 22753871
Immunohistochemical analysis on a malignant mesothelioma tissue microarray showed cytoplasmic staining in 28 of 33 samples for vimentin and strong cytoplasmic staining in14 and weak in 16 samples for ss-F1-ATPase. PMID: 22022619
Disturbed flow and hypercholesterolemia synergistically promote gamma/delta T-lymphocyte activation by the membrane translocation of ATPSbeta in endothelial cells. PMID: 21193741
Results suggested that the ectopic expression of ATP synthase is a consequence of translocation from the mitochondria. PMID: 20705594
Molecular and functional studies indicate that the interaction of G3BP1 with beta-F1 mRNA inhibits its translation at the initiation level, supporting a role for G3BP1 in the glycolytic switch that occurs in cancer. PMID: 20663914
ATP synthase beta is phosphorylated at multiple sites and shows abnormal phosphorylation at specific sites in insulin-resistant muscle PMID: 20012595
Findings of the present study support the hypothesis that down-regulation of the bioenergetic activity of mitochondria in human tumours is exerted by translation silencing of beta-F1-ATPase mRNA. PMID: 20028336
Co-immunoprecipitation experiments in the presence of alpha-methyl mannose verified the binding of Escherichia coli FimH to ATP synthase beta-subunit of human brain microvascular endothelial cells. PMID: 20067530
Ectopic beta-chain of ATP synthase is an apolipoprotein A-I receptor in hepatic HDL endocytosis PMID: 12511957
Membrane-bound ATP synthase functions as a receptor for CF6 and may have a previously unsuspected role in the genesis of hypertension by modulating the concentration of intracellular hydrogen. PMID: 16230521
adenosine/uridine (AU)-rich element-binding proteins TIA-1 (T-cell intracellular antigen-1), TIAR (TIA-1-related protein), and HuR (Hu antigen R) interact with the beta-F1-ATPase mRNA through an AU-rich sequence located to the 3'-UTR. PMID: 16890199
This short review summarizes demonstrations of ATP5B (complex 5 of oxidative phosphorylation) subunit that show its movement under stereochemical alterations known to be induced during the binding of ADP and synthesis of ATP. PMID: 16927672
cholesterol exposure increased the level of ATPS-beta, along with Cav-1 and cholesterol in caveolae. the ectopic localization of ATPS-beta may participate in the energy balance of cells in response to the change in intracellular cholesterol levels. PMID: 16996794
conclude that ATP synthase beta-subunit may have an important role in the glucolipotoxicity of islet cells PMID: 18284036
Low levels of beta-F1-ATPase are sensitive to combined platinum and 2-deoxy-D-glucose treatment in ovarian carcinoma. PMID: 19567816

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Subcellular Location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

Protein Families

ATPase alpha/beta chains family

Database Links

HGNC: 830

OMIM: 102910

KEGG: hsa:506

STRING: 9606.ENSP00000262030

UniGene: Hs.406510

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Code	CSB-RA216446A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: 293T whole cell lysate, HT29 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat Heart tissue, Mouse Heart tissue All lanes: ATP5F1B antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa IHC image of CSB-RA216446A0HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA216446A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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ATP5B Recombinant Monoclonal Antibody

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Target Background