Human Acetylcholinesterase,AChE ELISA Kit

Code CSB-E09670h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P22303
Lead Time 3-5 working days
Abbreviation ACHE
Protein Biological Process 1 Neurobiology
Target Name acetylcholinesterase (Yt blood group)
Alias ARACHE, N-ACHE, YT, OTTHUMP00000211347|OTTHUMP00000211349|OTTHUMP00000211356|acetylcholinesterase|apoptosis-related acetylcholinesterase
Species Homo sapiens (Human)
Protein Biological Process 3 Neurotransmitter degradation
Sample Types serum, plasma, tissue homogenates
Detection Range 0.94 mU/mL-60 mU/mL
Sensitivity 0.23 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.
HGNC 108
RGD 69313
MGI 87876
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %89
Range %84-93
1:200Average %95
Range %91-99
1:400Average %99
Range %95-104
1:800Average %92
Range %88-96
Recovery
The recovery of human AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-100
EDTA plasma (n=4)9186-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
602.462 2.546 2.504 2.385
302.176 2.243 2.210 2.091
151.904 1.905 1.905 1.786
7.51.439 1.473 1.456 1.337
3.750.855 0.824 0.840 0.721
1.880.478 0.459 0.469 0.350
0.940.267 0.249 0.258 0.139
00.120 0.117 0.119  
References
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Function Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
Subcellular Location Cell junction, synapse, Secreted, Cell membrane, Peripheral membrane protein, SUBCELLULAR LOCATION: Isoform T: Nucleus
Protein Families Type-B carboxylesterase/lipase family
Tissue Specificity Isoform H is highly expressed in erythrocytes.
Database Links

HGNC: 108

OMIM: 100740

KEGG: hsa:43

STRING: 9606.ENSP00000303211

UniGene: Hs.154495

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