Human Angiotensin Ⅱ Receptor 2 antibody,AT2R-Ab ELISA Kit

Code CSB-E11255h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Angiotensin Ⅱ Receptor 2 antibody,AT2R-Ab
Abbreviation
AT2R Ab
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
0.312 U/mL-20 U/mL
Sensitivity
0.078 U/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AT2R-Ab in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 82-105
1:2 Average % 94
Range % 84-100
1:4 Average % 98
Range % 92-101
1:8 Average % 94
Range % 82-105
Recovery
The recovery of human AT2R-Ab spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 83-105
EDTA plasma (n=4) 95 85-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/ml OD1 OD2 Average Corrected
20 2.635 2.659 2.647 2.501
10 1.963 1.927 1.945 1.799
5 1.296 1.287 1.292 1.146
2.5 0.771 0.748 0.760 0.614
1.25 0.468 0.492 0.480 0.334
0.625 0.346 0.351 0.349 0.203
0.312 0.255 0.246 0.251 0.105
0 0.147 0.145 0.146  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human AT2R Ab ELISA Kit was designed for the quantitative measurement of Human AT2R Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 U/mL-20 U/mL and the sensitivity is 0.078 U/mL.

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