Product Details

Target Name

anti-Mullerian hormone receptor, type II

Alternative Names

AMH type II receptor ELISA Kit; AMHR ELISA Kit; AMHR2 ELISA Kit; AMHR2_HUMAN ELISA Kit; Anti Mullerian hormone receptor; type II ELISA Kit; Anti-Muellerian hormone type II receptor ELISA Kit; Anti-Muellerian hormone type-2 receptor ELISA Kit; MGC141312 ELISA Kit; MIS type II receptor ELISA Kit; MISR2 ELISA Kit; MISRII ELISA Kit; MRII ELISA Kit; Muellerian hormone type 2 receptor ELISA Kit; Muellerian hormone type II receptor ELISA Kit; Muellerian inhibiting substance type II receptor ELISA Kit; Mullerian hormone receptor type II ELISA Kit; Mullerian inhibiting substance type II receptor ELISA Kit

Abbreviation

AMHR2

Uniprot No.

Q16671

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.32 ng/mL-12 ng/mL

Sensitivity

8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AMHR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-100
1:2	Average %	95
	Range %	90-105
1:4	Average %	92
	Range %	84-99
1:8	Average %	96
	Range %	90-101

Recovery

The recovery of human AMHR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	97	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
12	2.377	2.354	2.366	2.312
6.4	1.726	1.742	1.734	1.680
3.2	1.053	1.075	1.064	1.010
0.8	0.332	0.327	0.330	0.276
0.32	0.165	0.170	0.168	0.114
0	0.055	0.052	0.054

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human AMHR2 ELISA Kit was designed for the quantitative measurement of Human AMHR2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.32 ng/mL-12 ng/mL and the sensitivity is 8 pg/mL.

Target Background

Function
(From Uniprot)

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone.

Gene References into Functions

AMHR2 single nucleotide polymorphism is not associated with Endometriosis-associated infertility. PMID: 28831646
AMHRII 1749C > T and -482A > G genetic variants are associated with the ovarian response to standard gonadotropin stimulation, affecting mainly the follicular growth in IVF. PMID: 26933946
a result of VEGF misregulation, AMHR2 overexpression increases AMH binding, which may attenuate follicular or oocyte maturation. PMID: 27109000
-482A > G genotype not associated with estradiol levels, ovarian parameters, menstrual cycle length, or pregnancy outcomes in healthy Singapore women PMID: 26633196
A significant subset of GnRH neurons express the AMH receptor. PMID: 26753790
A significant portion of AMHRII was missing most of its extracellular domain (ECD) and was unfolded and retained in the endoplasmic reticulum. PMID: 25663701
study demonstrated for the first time that human placenta and fetal membranes express and co-localize Anti-Mullerian hormone(AMH) and Anti-Mullerian hormone Receptor II PMID: 25972076
AMHR2 rs11170555 and rs3741664 were positively associated with AMH, estradiol and FSH levels. PMID: 25790842
Data indicate that Muellerian inhibiting substance type II receptor (MISRII) is a promising target for the control of ovarian granulosa cell tumors (GCT) and epithelial ovarian cancers (EOC). PMID: 25517316
The possible involvement of AMHRII -482 A>G polymorphism on the malfunction of follicular development in Japanese women. PMID: 24271023
Antimullerian hormone receptor expression is increased in endometrium from patients with endometriosis. PMID: 24613539
Within primary ovarian insufficiency population, the AMH Ile(49)Ser and the AMHR2 -482A>G polymorphisms were not associated with age at the time of POI and PMID: 24146295
The role of the AMHR2 -482 A>G gene polymorphism in the pathogenesis of polycystic ovary syndrome was suggested by the association of the variant with risk. PMID: 23969185
likely molecular etiology was found in eight patients with persistent Mullerian duct syndrome. Four mutations in AMH and two in AMHR2 were identified. Three of them are novel mutations, c.556-2A>G, and p.Arg502Leu in AMH; and p.Gly323Ser in AMHR2. PMID: 23295284
Data suggest that up-regulation of AMHR2 and AMH expression in luteal granulosa cells of anovulatory women with polycystic ovary syndrome is due to rising levels of luteinizing hormone (LH) and/or reversal of down-regulation by LH. PMID: 23321213
The Mullerian inhibiting substance type 2 receptor suppresses tumorigenesis in testes with sustained beta-catenin signaling. PMID: 22962306
the diversity of clinical symptoms within sibship and lack of correlation between development of Mullerian derivatives and severity of molecular defects suggest highly variable penetrance of abnormal alleles PMID: 22584735
Association studies of common variants of AMHRII suggests that antimullerian hormone may regulate the primordial graafian follicle recruitment. PMID: 20362961
Review. The role of AMHR2 in gonadal development and its mutation in the persistent Mullerian duct syndrome is discussed. PMID: 12462075
binding domains recognized by a monoclonal antibody and the natural ligand PMID: 14750901
The observed association of the AMHR2 -482 A > G polymorphism with natural age at menopause suggests a role for AMH signaling in the usage of the primordial follicle pool in women. PMID: 17636279
MISIIR is highly expressed by a wide variety of gynecologic cancers, including cancers currently without effective systemic therapies. PMID: 17988723
Reduced AMH receptor type 2 is associated with Leydig cell tumours in multiple endocrine neoplasia type 1 PMID: 18310289
Non-epithelial malignant ovarian tumors showed stronger expression of anti-Mullerian hormone receptor type II than that of epithelial tumors. PMID: 19424576
Mutations of AMHR2 found in persistent Mullerian duct syndrome affect its ligand binding and cellular transport. PMID: 19457927
interacts with Mullerian inhibiting substance: an instructive developmental hormone with diagnostic and possible therapeutic applications. PMID: 11588147

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Involvement in disease

Persistent Muellerian duct syndrome 2 (PMDS2)

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfamily

Database Links

HGNC: 465

OMIM: 261550

KEGG: hsa:269

STRING: 9606.ENSP00000257863

UniGene: Hs.659889

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Human Anti-Muellerian hormone type-2 receptor(AMHR2) ELISA kit

Product Details

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Target Background