Human Anti-Muellerian hormone type-2 receptor(AMHR2) ELISA kit

Instructions
Code CSB-EL001667HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name anti-Mullerian hormone receptor, type II
Alternative Names AMH type II receptor ELISA Kit; AMHR ELISA Kit; AMHR2 ELISA Kit; AMHR2_HUMAN ELISA Kit; Anti Mullerian hormone receptor; type II ELISA Kit; Anti-Muellerian hormone type II receptor ELISA Kit; Anti-Muellerian hormone type-2 receptor ELISA Kit; MGC141312 ELISA Kit; MIS type II receptor ELISA Kit; MISR2 ELISA Kit; MISRII ELISA Kit; MRII ELISA Kit; Muellerian hormone type 2 receptor ELISA Kit; Muellerian hormone type II receptor ELISA Kit; Muellerian inhibiting substance type II receptor ELISA Kit; Mullerian hormone receptor type II ELISA Kit; Mullerian inhibiting substance type II receptor ELISA Kit
Abbreviation AMHR2
Uniprot No. Q16671
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.32 ng/mL-12 ng/mL
Sensitivity 8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AMHR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 86-100
1:2 Average % 95
Range % 90-105
1:4 Average % 92
Range % 84-99
1:8 Average % 96
Range % 90-101
Recovery
The recovery of human AMHR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 92-103
EDTA plasma (n=4) 97 93-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
12 2.377 2.354 2.366 2.312
6.4 1.726 1.742 1.734 1.680
3.2 1.053 1.075 1.064 1.010
0.8 0.332 0.327 0.330 0.276
0.32 0.165 0.170 0.168 0.114
0 0.055 0.052 0.054  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone.
Gene References into Functions
  1. AMHR2 single nucleotide polymorphism is not associated with Endometriosis-associated infertility. PMID: 28831646
  2. AMHRII 1749C > T and -482A > G genetic variants are associated with the ovarian response to standard gonadotropin stimulation, affecting mainly the follicular growth in IVF. PMID: 26933946
  3. a result of VEGF misregulation, AMHR2 overexpression increases AMH binding, which may attenuate follicular or oocyte maturation. PMID: 27109000
  4. -482A > G genotype not associated with estradiol levels, ovarian parameters, menstrual cycle length, or pregnancy outcomes in healthy Singapore women PMID: 26633196
  5. A significant subset of GnRH neurons express the AMH receptor. PMID: 26753790
  6. A significant portion of AMHRII was missing most of its extracellular domain (ECD) and was unfolded and retained in the endoplasmic reticulum. PMID: 25663701
  7. study demonstrated for the first time that human placenta and fetal membranes express and co-localize Anti-Mullerian hormone(AMH) and Anti-Mullerian hormone Receptor II PMID: 25972076
  8. AMHR2 rs11170555 and rs3741664 were positively associated with AMH, estradiol and FSH levels. PMID: 25790842
  9. Data indicate that Muellerian inhibiting substance type II receptor (MISRII) is a promising target for the control of ovarian granulosa cell tumors (GCT) and epithelial ovarian cancers (EOC). PMID: 25517316
  10. The possible involvement of AMHRII -482 A>G polymorphism on the malfunction of follicular development in Japanese women. PMID: 24271023
  11. Antimullerian hormone receptor expression is increased in endometrium from patients with endometriosis. PMID: 24613539
  12. Within primary ovarian insufficiency population, the AMH Ile(49)Ser and the AMHR2 -482A>G polymorphisms were not associated with age at the time of POI and PMID: 24146295
  13. The role of the AMHR2 -482 A>G gene polymorphism in the pathogenesis of polycystic ovary syndrome was suggested by the association of the variant with risk. PMID: 23969185
  14. likely molecular etiology was found in eight patients with persistent Mullerian duct syndrome. Four mutations in AMH and two in AMHR2 were identified. Three of them are novel mutations, c.556-2A>G, and p.Arg502Leu in AMH; and p.Gly323Ser in AMHR2. PMID: 23295284
  15. Data suggest that up-regulation of AMHR2 and AMH expression in luteal granulosa cells of anovulatory women with polycystic ovary syndrome is due to rising levels of luteinizing hormone (LH) and/or reversal of down-regulation by LH. PMID: 23321213
  16. The Mullerian inhibiting substance type 2 receptor suppresses tumorigenesis in testes with sustained beta-catenin signaling. PMID: 22962306
  17. the diversity of clinical symptoms within sibship and lack of correlation between development of Mullerian derivatives and severity of molecular defects suggest highly variable penetrance of abnormal alleles PMID: 22584735
  18. Association studies of common variants of AMHRII suggests that antimullerian hormone may regulate the primordial graafian follicle recruitment. PMID: 20362961
  19. Review. The role of AMHR2 in gonadal development and its mutation in the persistent Mullerian duct syndrome is discussed. PMID: 12462075
  20. binding domains recognized by a monoclonal antibody and the natural ligand PMID: 14750901
  21. The observed association of the AMHR2 -482 A > G polymorphism with natural age at menopause suggests a role for AMH signaling in the usage of the primordial follicle pool in women. PMID: 17636279
  22. MISIIR is highly expressed by a wide variety of gynecologic cancers, including cancers currently without effective systemic therapies. PMID: 17988723
  23. Reduced AMH receptor type 2 is associated with Leydig cell tumours in multiple endocrine neoplasia type 1 PMID: 18310289
  24. Non-epithelial malignant ovarian tumors showed stronger expression of anti-Mullerian hormone receptor type II than that of epithelial tumors. PMID: 19424576
  25. Mutations of AMHR2 found in persistent Mullerian duct syndrome affect its ligand binding and cellular transport. PMID: 19457927
  26. interacts with Mullerian inhibiting substance: an instructive developmental hormone with diagnostic and possible therapeutic applications. PMID: 11588147

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Involvement in disease Persistent Muellerian duct syndrome 2 (PMDS2)
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfamily
Database Links

HGNC: 465

OMIM: 261550

KEGG: hsa:269

STRING: 9606.ENSP00000257863

UniGene: Hs.659889

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