Human Antibacterial Peptide LL-37 ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Name: Human Antibacterial Peptide LL-37 ELISA Kit
Brand: CUSABIO
SKU: CSB-E14948h

Product Details

Target Name

Antibacterial Peptide LL-37

Abbreviation

LL-37

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LL-37 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	85
	Range %	80-92
1:10	Average %	98
	Range %	91-105
1:20	Average %	98
	Range %	92-110
1:40	Average %	93
	Range %	86-98

Recovery

The recovery of human LL-37 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	104	92-112

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.420	2.468	2.444	2.246
5	1.875	1.895	1.885	1.687
2.5	1.358	1.378	1.368	1.170
1.25	0.941	0.956	0.949	0.751
0.625	0.552	0.589	0.571	0.373
0.312	0.431	0.453	0.442	0.244
0.156	0.378	0.389	0.384	0.186
0	0.192	0.204	0.198

Materials provided

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human antibacterial peptide LL-37 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled antibacterial peptide LL-37 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human LL-37 ELISA Kit was designed for the quantitative measurement of Human LL-37 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Customer Reviews and Q&A

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■ Q&A

We want to use pbs buffer 7.4 as sample instead of serum/plasma etc. Would this affect the results? Can you give us any protocol/suggestion? We need an urgent reply:CSB-E14948h Human Antibacterial Peptide LL-37 ELISA Kit and CSB-E14187h Human β-defensins 3 ELISA Kit.

Thanks for your inquiry.Sorry we did not ever tested PBS 7.4 as sample and we are not sure how it will go on.PBS is mainly composed of phosphate.
Normally,there is no detection target in PBS.May I know if you were trying to say that the customer want to use PBS 7.4 as the sample diluent,not the mentioned serum,plasma sample?The kit is equiped with sample diluent in it and we suggest the customer use the sample diluent in the kit directly.
If further questions,pls let us know.

I would like to test this ELISA for LL37. Would this ELISA can be used for the quantification of full length cathelicidin and LL37?

Thanks for your inquiry. This kit detects LL-37.Any question, pls feel free to contact me.

Code	CSB-E14948h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.