Human Antibacterial Peptide LL-37 ELISA Kit

Code CSB-E14948h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human LL-37 ELISA Kit was designed for the quantitative measurement of Human LL-37 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Name Antibacterial Peptide LL-37
Abbreviation LL-37
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LL-37 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 85  
Range % 80-92  
1:10 Average % 98  
Range % 91-105  
1:20 Average % 98  
Range % 92-110  
1:40 Average % 93  
Range % 86-98  
Recovery
The recovery of human LL-37 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
EDTA plasma (n=4) 104 92-112  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.420 2.468 2.444 2.246  
5 1.875 1.895 1.885 1.687  
2.5 1.358 1.378 1.368 1.170  
1.25 0.941 0.956 0.949 0.751  
0.625 0.552 0.589 0.571 0.373  
0.312 0.431 0.453 0.442 0.244  
0.156 0.378 0.389 0.384 0.186  
0 0.192 0.204 0.198    
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human antibacterial peptide LL-37 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled antibacterial peptide LL-37 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

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 Q&A
Q:

We want to use pbs buffer 7.4 as sample instead of serum/plasma etc. Would this affect the results? Can you give us any protocol/suggestion? We need an urgent reply:CSB-E14948h Human Antibacterial Peptide LL-37 ELISA Kit and CSB-E14187h Human β-defensins 3 ELISA Kit.

A:
Thanks for your inquiry.Sorry we did not ever tested PBS 7.4 as sample and we are not sure how it will go on.PBS is mainly composed of phosphate.
Normally,there is no detection target in PBS.May I know if you were trying to say that the customer want to use PBS 7.4 as the sample diluent,not the mentioned serum,plasma sample?The kit is equiped with sample diluent in it and we suggest the customer use the sample diluent in the kit directly.
If further questions,pls let us know.
Q:

I would like to test this ELISA for LL37. Would this ELISA can be used for the quantification of full length cathelicidin and LL37?

A:
Thanks for your inquiry. This kit detects LL-37.Any question, pls feel free to contact me.
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Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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