Human Asporin(ASPN) ELISA kit

Instructions
Code CSB-EL002230HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name asporin
Alternative Names ASPN ELISA Kit; ASPN protein ELISA Kit; ASPN_HUMAN ELISA Kit; Asporin (LRR class 1) ELISA Kit; Asporin ELISA Kit; Asporin proteoglycan ELISA Kit; FLJ20129 ELISA Kit; LRR class 1 ELISA Kit; Periodontal ligament associated protein 1 ELISA Kit; Periodontal ligament-associated protein 1 ELISA Kit; PLAP 1 ELISA Kit; PLAP-1 ELISA Kit; PLAP1 ELISA Kit; SLRR 1C ELISA Kit; SLRR1C ELISA Kit; Small leucine rich protein 1C ELISA Kit
Abbreviation ASPN
Uniprot No. Q9BXN1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 34.37 pg/mL-2200 pg/mL
Sensitivity 8.59 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ASPN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 102    
Range % 93-108    
1:2 Average % 97    
Range % 89-101    
1:4 Average % 93    
Range % 88-97    
1:8 Average % 99    
Range % 90-103    
Recovery
The recovery of human ASPN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 100 94-104    
EDTA plasma (n=4) 92 85-97    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
2200 2.059 2.000 2.030 1.916    
1100 1.451 1.378 1.415 1.301    
550 0.929 0.953 0.941 0.827    
275 0.659 0.650 0.655 0.541    
137.5 0.339 0.344 0.342 0.228    
68.75 0.261 0.249 0.255 0.141    
34.37 0.182 0.178 0.180 0.066    
0 0.116 0.112 0.114      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.
Gene References into Functions
  1. D-repeat of ASPN gene is mainly associated with male patients. The D13 polymorphism plays a protective role for osteoarthritis in Caucasians male individuals while D14 plays a risk factor for knee osteoarthritis in male patients. PMID: 30407347
  2. Suggest that the D14 allele of the ASPN polymorphism could exert an influence on primary osteoarthritis of the knee etiology in a Mexican Mestizo population. PMID: 26620055
  3. ASPN D-repeat polymorphism is not associated with an increased Knee Osteoarthritis risk. PMID: 28889984
  4. Meta-analysis result based on previously published studies demonstrated that the ASPN D13 allele was a protective factor for OA of knee, hip, and hand. For D14 and D15 allele, meta-analysis did not demonstrate statistically significant association. PMID: 29561445
  5. higher expression of asporin was noticed in CRC tissues and it was correlated with later clinical stage of the patients. Asporin promoted the migration and invasion of the tumor cells partially through an EGFR/Src/cortactin- signaling pathway. PMID: 27705916
  6. ASPN D15 is associated with increased risk of symmetrical hand osteoarthritis particularly in individuals with low variation in work tasks. PMID: 29233086
  7. we found that asporin can be downregulated by bone morphogenetic protein 4 in Hs578T cells and its upregulation may be facilited by serum-free cultivation or by three dimensional growth PMID: 27409832
  8. Asporin promotes epithelial mesenchymal transformation, invasion, and migration of pancreatic cancer cellss by activating CD44-AKT/ERK-NF-kappaB pathway in paracrine and autocrine manner. PMID: 28400334
  9. High ASPN expression in stroma is associated with prostate cancer progression. PMID: 28152543
  10. To test the associations of ASPN variations with risk of subsequent oncologic outcomes. PMID: 26446945
  11. Collectively, our findings indicate that ASPN is upregulated and plays an oncogenic role in gastric cancer progression and metastasis by influencing the EGFR signaling pathway. PMID: 25673058
  12. Our results showed that ASPN rs13301537 T to C change and variant C genotype may contribute to knee OA risk in a Chinese Han population. PMID: 25030405
  13. this is the first case-control study in Mexican women that suggests that menopause and the D-repeat polymorphism in the ASPN gene are associated with knee OA PMID: 26016288
  14. Osteomodulin, osteoglycin, and asporin appear to be distinctly regulated in osteoarthritis labrum compared to OA cartilage. PMID: 25371314
  15. Asporin may represent a new therapeutic target molecule for the development of drugs aimed at manipulating the cancer microenvironment. PMID: 24441039
  16. This meta-analysis shows that the ASPN D14, D13, and D15 alleles are not associated with the development of osteoarthritis in Europeans and Asians. [Meta-Analysis] PMID: 24306268
  17. A meta-analysis suggest that the D-repeat of asporin gene (ASPN) may not be a major susceptibility locus in the Caucasian and Asian populations with knee osteoarthritis. PMID: 23942062
  18. polymorphisms within the ASPN gene could influence knee osteoarthritis susceptibility PMID: 23733110
  19. D14-PLAP-1 suppressed BMP-2 signal transduction more efficiently than D13-PLAP-1; stronger affinity of D14-PLAP-1 protein to BMP-2 compared with D13-PLAP-1 protein. D repeat polymorphism of PLAP-1/asporin has influence on functions of PDL cells. PMID: 24453179
  20. Our data suggest that the D15 asporin allele could be considered a knee osteoarthritis risk allele significant only for women in the Iranian population. PMID: 24078942
  21. The asporin-encoding gene is a promising candidate as a susceptibility gene for osteoarthritis and degenerative disc disease. [Review] PMID: 24003854
  22. Asporin is associated with hand osteoarthritis progression. PMID: 23357225
  23. mir-101 and mir-21 target PLAP-1 to regulate its expression during osteogenic differentiation of PDLCs. PMID: 22367347
  24. Our results show an obvious association between the D repeat polymorphism of ASPN and Developmental dysplasia of the hip PMID: 21329514
  25. Data indicate that the expression of ASPN gene is finely regulated in cartilage and suggest a major role of Sp1. PMID: 21528154
  26. ASPN plays positive roles in the mineralization of dental pulp stem cells and predentin to dentin. PMID: 21413025
  27. The ratio of Asporin to TGF-beta1 (transforming growth factor-beta1) mRNA in patients with severe cartilage damage was higher than that in osteoartritis patients with mild cartilage damage PMID: 19997821
  28. Association of an asporin repeat polymorphism with ankylosing spondylitis in Han Chinese population: a case-control study, is reported. PMID: 20144272
  29. these findings provide another functional link between extracellular matrix proteins, TGF-beta activity and disease, suggesting new therapeutic strategies for osteoarthritis. PMID: 15640800
  30. No significant differences were observed in any of the multiple comparisons performed in osteoarthritis and controls PMID: 16542493
  31. expression may be associated with the process of cytodifferentiation of periodontal ligament cells PMID: 16632759
  32. Association of the D14 allele with knee osteoarthritis susceptibility in Han Chinese. PMID: 17024313
  33. the association of the ASPN D14 allele and knee OA has global relevance [meta-analysis] PMID: 17517696
  34. This study further highlighted the significance of asporin in osteoarthritis. PMID: 17603749
  35. Characterization of the human ASPN promoter region revealed a region from -126 to -82 that is sufficient for full promoter activity; however, TGF-beta1 failed to increase activity through the ASPN promoter. PMID: 17804408
  36. describe mechanisms for asporin function and regulation in human articular cartilage; asporin blocks chondrogenesis and inhibits TGF-beta1-induced expression of matrix genes and the resulting chondrocyte phenotypes. PMID: 17827158
  37. The frequency of the aspartic acid repeat polymorphism was examined. These results suggest that asporin may play a role in OA susceptibility of the knee in the Korean female population. PMID: 18178444
  38. ASPN is a lumbar-disc degeneration (LDD) gene in Asians, and common risk factors may be considered for osteoarthritis and LDD. PMID: 18304494
  39. Asporin is up-regulated in disease states. It binds to various growth factors, including TGF-beta and BMP-2, and negatively regulates their activity. By inhibiting binding of TGF-beta1 to its type II receptor, asporin forms a functional feedback loop. PMID: 18336287
  40. These data suggest that polymorphisms within ASPN are not a major influence in susceptibility to hand or knee OA in US Caucasians. PMID: 18434216
  41. In the discs of Caucasian subjects there was greatest expression of asporin in the more degenerate human discs in vivo. PMID: 19327154
  42. Asporin has a role in osteoblast-driven collagen biomineralization activity. PMID: 19589127

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Involvement in disease Osteoarthritis 3 (OS3); Intervertebral disc disease (IDD)
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Small leucine-rich proteoglycan (SLRP) family, SLRP class I subfamily
Tissue Specificity Higher levels in osteoarthritic articular cartilage, aorta, uterus. Moderate expression in small intestine, heart, liver, bladder, ovary, stomach, and in the adrenal, thyroid, and mammary glands. Low expression in trachea, bone marrow, and lung. Colocaliz
Database Links

HGNC: 14872

OMIM: 603932

KEGG: hsa:54829

STRING: 9606.ENSP00000364694

UniGene: Hs.435655

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