Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ASPN

Uniprot No.

Q9BXN1

Research Area

Signal Transduction

Alternative Names

ASPN; ASPN protein; ASPN_HUMAN; Asporin (LRR class 1); Asporin; Asporin proteoglycan; FLJ20129; LRR class 1; Periodontal ligament associated protein 1; Periodontal ligament-associated protein 1; PLAP 1; PLAP-1; PLAP1; SLRR 1C; SLRR1C; Small leucine rich protein 1C

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

33-380aa

Target Protein Sequence

DMEDTDDDDDDDDDDDDDDEDNSLFPTREPRSHFFPFDLFPMCPFGCQCYSRVVHCSDLGLTSVPTNIPFDTRMLDLQNNKIKEIKENDFKGLTSLYGLILNNNKLTKIHPKAFLTTKKLRRLYLSHNQLSEIPLNLPKSLAELRIHENKVKKIQKDTFKGMNALHVLEMSANPLDNNGIEPGAFEGVTVFHIRIAEAKLTSVPKGLPPTLLELHLDYNKISTVELEDFKRYKELQRLGLGNNKITDIENGSLANIPRVREIHLENNKLKKIPSGLPELKYLQIIFLHSNSIARVGVNDFCPTVPKMKKSLYSAISLFNNPVKYWEMQPATFRCVLSRMSVQLGNFGM
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

55.7kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression of recombinant human ASPN protein includes the construction of the expression vector containing the recombinant DNA and the transformation of the expression vector into the E.coli, which provides a variety of macromolecules and components required for transcription and translation. The recombinant DNA was formed by fusing the N-terminal 6xHis-SUMO tag sequence to the designated sequence encoding the 33-380aa of the human ASPN protein. This N-terminal 6xHis-SUMO-tagged recombinant human ASPN protein is also characterized by high purity, >90%. Under SDS-PAGE condition, this recombinant ASPN protein migrated to the band of about 55 kDa molecular weight.

It is well-documented that ASPN was upregulated in different stages of gastric cancer (GC),and associated with a poor prognosis and could serve as a potential prognostic biomarker in colorectal cancer. Further data suggested that it promotes the migration and invasion of colorectal cancer cells via TGFβ/Smad2/3 pathway. Asporin as an ECM protein, which belongs to a small leucine-rich proteoglycan family. In ovarian cancinoma, researchers determined that ASPN is highly expressed in CAFs of high grade serous ovarian cancinoma patients and predicts poor prognosis. Moreover, ASPN may be a potential promising biomarker for heart failure. ASPN protein was reported to significantly increased in ischemic cardiomyopathy and dilated cardiomyopathy left ventricular samples. ASPN protein was significantly increased in ICM and DCM left ventricular samples. In additon, asporin levels will be increased as a result of degeneration in advanced TMJ joint disease with tissue damage.

Target Background

Function

Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins. Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.

Gene References into Functions

D-repeat of ASPN gene is mainly associated with male patients. The D13 polymorphism plays a protective role for osteoarthritis in Caucasians male individuals while D14 plays a risk factor for knee osteoarthritis in male patients. PMID: 30407347
Suggest that the D14 allele of the ASPN polymorphism could exert an influence on primary osteoarthritis of the knee etiology in a Mexican Mestizo population. PMID: 26620055
ASPN D-repeat polymorphism is not associated with an increased Knee Osteoarthritis risk. PMID: 28889984
Meta-analysis result based on previously published studies demonstrated that the ASPN D13 allele was a protective factor for OA of knee, hip, and hand. For D14 and D15 allele, meta-analysis did not demonstrate statistically significant association. PMID: 29561445
higher expression of asporin was noticed in CRC tissues and it was correlated with later clinical stage of the patients. Asporin promoted the migration and invasion of the tumor cells partially through an EGFR/Src/cortactin- signaling pathway. PMID: 27705916
ASPN D15 is associated with increased risk of symmetrical hand osteoarthritis particularly in individuals with low variation in work tasks. PMID: 29233086
we found that asporin can be downregulated by bone morphogenetic protein 4 in Hs578T cells and its upregulation may be facilited by serum-free cultivation or by three dimensional growth PMID: 27409832
Asporin promotes epithelial mesenchymal transformation, invasion, and migration of pancreatic cancer cellss by activating CD44-AKT/ERK-NF-kappaB pathway in paracrine and autocrine manner. PMID: 28400334
High ASPN expression in stroma is associated with prostate cancer progression. PMID: 28152543
To test the associations of ASPN variations with risk of subsequent oncologic outcomes. PMID: 26446945
Collectively, our findings indicate that ASPN is upregulated and plays an oncogenic role in gastric cancer progression and metastasis by influencing the EGFR signaling pathway. PMID: 25673058
Our results showed that ASPN rs13301537 T to C change and variant C genotype may contribute to knee OA risk in a Chinese Han population. PMID: 25030405
this is the first case-control study in Mexican women that suggests that menopause and the D-repeat polymorphism in the ASPN gene are associated with knee OA PMID: 26016288
Osteomodulin, osteoglycin, and asporin appear to be distinctly regulated in osteoarthritis labrum compared to OA cartilage. PMID: 25371314
Asporin may represent a new therapeutic target molecule for the development of drugs aimed at manipulating the cancer microenvironment. PMID: 24441039
This meta-analysis shows that the ASPN D14, D13, and D15 alleles are not associated with the development of osteoarthritis in Europeans and Asians. [Meta-Analysis] PMID: 24306268
A meta-analysis suggest that the D-repeat of asporin gene (ASPN) may not be a major susceptibility locus in the Caucasian and Asian populations with knee osteoarthritis. PMID: 23942062
polymorphisms within the ASPN gene could influence knee osteoarthritis susceptibility PMID: 23733110
D14-PLAP-1 suppressed BMP-2 signal transduction more efficiently than D13-PLAP-1; stronger affinity of D14-PLAP-1 protein to BMP-2 compared with D13-PLAP-1 protein. D repeat polymorphism of PLAP-1/asporin has influence on functions of PDL cells. PMID: 24453179
Our data suggest that the D15 asporin allele could be considered a knee osteoarthritis risk allele significant only for women in the Iranian population. PMID: 24078942
The asporin-encoding gene is a promising candidate as a susceptibility gene for osteoarthritis and degenerative disc disease. [Review] PMID: 24003854
Asporin is associated with hand osteoarthritis progression. PMID: 23357225
mir-101 and mir-21 target PLAP-1 to regulate its expression during osteogenic differentiation of PDLCs. PMID: 22367347
Our results show an obvious association between the D repeat polymorphism of ASPN and Developmental dysplasia of the hip PMID: 21329514
Data indicate that the expression of ASPN gene is finely regulated in cartilage and suggest a major role of Sp1. PMID: 21528154
ASPN plays positive roles in the mineralization of dental pulp stem cells and predentin to dentin. PMID: 21413025
The ratio of Asporin to TGF-beta1 (transforming growth factor-beta1) mRNA in patients with severe cartilage damage was higher than that in osteoartritis patients with mild cartilage damage PMID: 19997821
Association of an asporin repeat polymorphism with ankylosing spondylitis in Han Chinese population: a case-control study, is reported. PMID: 20144272
these findings provide another functional link between extracellular matrix proteins, TGF-beta activity and disease, suggesting new therapeutic strategies for osteoarthritis. PMID: 15640800
No significant differences were observed in any of the multiple comparisons performed in osteoarthritis and controls PMID: 16542493
expression may be associated with the process of cytodifferentiation of periodontal ligament cells PMID: 16632759
Association of the D14 allele with knee osteoarthritis susceptibility in Han Chinese. PMID: 17024313
the association of the ASPN D14 allele and knee OA has global relevance [meta-analysis] PMID: 17517696
This study further highlighted the significance of asporin in osteoarthritis. PMID: 17603749
Characterization of the human ASPN promoter region revealed a region from -126 to -82 that is sufficient for full promoter activity; however, TGF-beta1 failed to increase activity through the ASPN promoter. PMID: 17804408
describe mechanisms for asporin function and regulation in human articular cartilage; asporin blocks chondrogenesis and inhibits TGF-beta1-induced expression of matrix genes and the resulting chondrocyte phenotypes. PMID: 17827158
The frequency of the aspartic acid repeat polymorphism was examined. These results suggest that asporin may play a role in OA susceptibility of the knee in the Korean female population. PMID: 18178444
ASPN is a lumbar-disc degeneration (LDD) gene in Asians, and common risk factors may be considered for osteoarthritis and LDD. PMID: 18304494
Asporin is up-regulated in disease states. It binds to various growth factors, including TGF-beta and BMP-2, and negatively regulates their activity. By inhibiting binding of TGF-beta1 to its type II receptor, asporin forms a functional feedback loop. PMID: 18336287
These data suggest that polymorphisms within ASPN are not a major influence in susceptibility to hand or knee OA in US Caucasians. PMID: 18434216
In the discs of Caucasian subjects there was greatest expression of asporin in the more degenerate human discs in vivo. PMID: 19327154
Asporin has a role in osteoblast-driven collagen biomineralization activity. PMID: 19589127

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Involvement in disease

Osteoarthritis 3 (OS3); Intervertebral disc disease (IDD)

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Families

Small leucine-rich proteoglycan (SLRP) family, SLRP class I subfamily

Tissue Specificity

Higher levels in osteoarthritic articular cartilage, aorta, uterus. Moderate expression in small intestine, heart, liver, bladder, ovary, stomach, and in the adrenal, thyroid, and mammary glands. Low expression in trachea, bone marrow, and lung. Colocaliz

Database Links

HGNC: 14872

OMIM: 603932

KEGG: hsa:54829

STRING: 9606.ENSP00000364694

UniGene: Hs.435655

Code	CSB-EP002230HU
Abbreviation	Recombinant Human ASPN protein
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Asporin (ASPN)

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Target Background

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