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| Code | CSB-E09698h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human AZU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-91 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-107 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 82-98 | |||||
| The recovery of human AZU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 80-98 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human Azurocidin (AZU1) ELISA Kit is used to quantitatively measure human AZU1 concentrations in serum, plasma, cell culture supernates, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of AZU1 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
AZU1, also known as CAP37 or heparin-binding protein (HBP), is an antimicrobial and chemotactic protein that is part of the innate defenses of human neutrophils. It is released from PMN secretory vesicles or primary granules and acts as a chemoattractant and activator of monocyte and macrophages. In neutrophils, AZU1 neutrophil elastase, and cathepsin G mainly function to defend the host against microbial pathogens and to mediate inflammation. Additionally, AZU1 is an inactive serine protease homolog with binding sites for diverse ligands including heparin and the bovine pancreatic trypsin inhibitor (BPTI).
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The instruction in the ELISA Kit said that the standard should be used within 4 hours after reconstruction, but the Recombinant Human Azurocidin, which is on the item of related products, could be used in one week. I wonder whether the standard can be used after 4 hours or not.
Usually, we do not write it in the instruction. The dissolved standard without any contaminations could be stored for one week. But if the dissolved standard is diluted with different gradients should be used in 4 hours.
