Human Azurocidin/Heparin Binding Protein(AZU/HBP) ELISA Kit

Code CSB-E09698h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

The human Azurocidin (AZU1) ELISA Kit is used to quantitatively measure human AZU1 concentrations in serum, plasma, cell culture supernates, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of AZU1 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

AZU1, also known as CAP37 or heparin-binding protein (HBP), is an antimicrobial and chemotactic protein that is part of the innate defenses of human neutrophils. It is released from PMN secretory vesicles or primary granules and acts as a chemoattractant and activator of monocyte and macrophages. In neutrophils, AZU1 neutrophil elastase, and cathepsin G mainly function to defend the host against microbial pathogens and to mediate inflammation. Additionally, AZU1 is an inactive serine protease homolog with binding sites for diverse ligands including heparin and the bovine pancreatic trypsin inhibitor (BPTI).

Target Name azurocidin 1
Abbreviation AZU1
Uniprot No. P20160
Species Homo sapiens (Human)
Sample Types serum, urine, tissue homogenates, cell culture supernates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AZU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 80-91  
1:2 Average % 95  
Range % 90-100  
1:4 Average % 98  
Range % 92-107  
1:8 Average % 92  
Range % 82-98  
Recovery
The recovery of human AZU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 80-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.898 2.682 2.790 2.686  
250 2.531 2.486 2.509 2.405  
125 2.147 2.045 2.096 1.992  
62.5 1.602 1.501 1.552 1.448  
31.2 0.887 0.872 0.880 0.776  
15.6 0.504 0.493 0.499 0.395  
7.8 0.278 0.256 0.267 0.163  
0 0.107 0.100 0.104    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
Subcellular Location Cytoplasmic granule membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families Peptidase S1 family, Elastase subfamily
Database Links

HGNC: 913

OMIM: 162815

KEGG: hsa:566

STRING: 9606.ENSP00000233997

UniGene: Hs.72885

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