The human Azurocidin (AZU1) ELISA Kit is used to quantitatively measure human AZU1 concentrations in serum, plasma, cell culture supernates, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of AZU1 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
AZU1, also known as CAP37 or heparin-binding protein (HBP), is an antimicrobial and chemotactic protein that is part of the innate defenses of human neutrophils. It is released from PMN secretory vesicles or primary granules and acts as a chemoattractant and activator of monocyte and macrophages. In neutrophils, AZU1 neutrophil elastase, and cathepsin G mainly function to defend the host against microbial pathogens and to mediate inflammation. Additionally, AZU1 is an inactive serine protease homolog with binding sites for diverse ligands including heparin and the bovine pancreatic trypsin inhibitor (BPTI).