Human Bromodomain testis-specific protein(BRDT) ELISA kit

Code CSB-EL002807HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
bromodomain, testis-specific
Alternative Names
BRDT ELISA Kit; Bromodomain testis-specific protein ELISA Kit; Cancer/testis antigen 9 ELISA Kit; CT9 ELISA Kit; RING3-like protein ELISA Kit
Abbreviation
BRDT
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Epigenetics and Nuclear Signaling
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human BRDT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 92    
Range % 88-96    
1:2 Average % 87    
Range % 82-91    
1:4 Average % 105    
Range % 101-113    
1:8 Average % 97    
Range % 92-101    
Recovery
The recovery of human BRDT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 90-100    
EDTA plasma (n=4) 84 80-88    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
4000 2.751 2.593 2.672 2.541    
2000 1.875 1.758 1.817 1.686    
1000 1.040 1.070 1.055 0.924    
500 0.592 0.611 0.602 0.471    
250 0.364 0.349 0.357 0.226    
125 0.239 0.227 0.233 0.102    
62.5 0.158 0.165 0.162 0.031    
0 0.127 0.134 0.131      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human BRDT ELISA Kit was designed for the quantitative measurement of Human BRDT protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Target Background

Function
(From Uniprot)
Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
Gene References into Functions
  1. FBP1 modulates the sensitivity of pancreatic cancer cells to BET inhibitors by decreasing the expression of c-Myc. These findings highlight FBP1 could be used as a therapeutic niche for patient-tailored therapies PMID: 30201002
  2. The bromodomain and extraterminal domain (BET) family consists of BRDT, BRD2, BRD3, and BRD4, each containing 2 bromodomains located N-terminal to extraterminal domain, which is located near C-terminus. Data suggest that 10 distinct acylations participate in binding of BET family proteins to histone 4 (oligopeptide fragments used here); C-terminal bromodomains do not cooperatively bind multiple acylation sites. PMID: 28945351
  3. The affected patient carries a homozygous bromodomain, testis-specific protein (BRDT) mutation, while the patient's father, mother and elder brother all carry heterozygous BRDT mutations. PMID: 28199965
  4. considered potential associations of 14 single nucleotide polymorphisms (SNPs) in RNF8 and BRDT genes in Chinese patients with non-obstructive azoospermia PMID: 25374327
  5. assessment of rs3088232 frequency in large group of non-obstructive azoospermia men and fertile controls demonstrated no significant difference between them; conclude that testicular impairments observed were not a consequence of BRDT gene mutation PMID: 24865796
  6. In human, BRDT is the only BET gene expressed exclusively in testicular germ cells. PMID: 22035730
  7. Suggest that reduced histone methylation in the promoter of BRDT may be associated with increased transcript levels in subfertile patients. PMID: 20538714
  8. BRDT-NY gene is an alternatively spliced variant that may have an important role in the process of spermatogenesis and may be correlated with male infertility PMID: 15647849

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Involvement in disease
Spermatogenic failure 21 (SPGF21)
Subcellular Location
Nucleus.
Tissue Specificity
Testis-specific. A 3-fold higher expression is seen in adult testis than in embryo testis. Expression seems to be correlated with histone H4 hyperacetylation during the haploid phase of spermatogenesis (spermiogenesis). No expression, or very low expressi
Database Links

HGNC: 1105

OMIM: 602144

KEGG: hsa:676

STRING: 9606.ENSP00000354568

UniGene: Hs.482520

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