Human Bromodomain testis-specific protein(BRDT) ELISA kit

Instructions
Code CSB-EL002807HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name bromodomain, testis-specific
Alternative Names BRDT ELISA Kit; Bromodomain testis-specific protein ELISA Kit; Cancer/testis antigen 9 ELISA Kit; CT9 ELISA Kit; RING3-like protein ELISA Kit
Abbreviation BRDT
Uniprot No. Q58F21
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human BRDT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 92    
Range % 88-96    
1:2 Average % 87    
Range % 82-91    
1:4 Average % 105    
Range % 101-113    
1:8 Average % 97    
Range % 92-101    
Recovery
The recovery of human BRDT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 90-100    
EDTA plasma (n=4) 84 80-88    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
4000 2.751 2.593 2.672 2.541    
2000 1.875 1.758 1.817 1.686    
1000 1.040 1.070 1.055 0.924    
500 0.592 0.611 0.602 0.471    
250 0.364 0.349 0.357 0.226    
125 0.239 0.227 0.233 0.102    
62.5 0.158 0.165 0.162 0.031    
0 0.127 0.134 0.131      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis
Gene References into Functions
  1. FBP1 modulates the sensitivity of pancreatic cancer cells to BET inhibitors by decreasing the expression of c-Myc. These findings highlight FBP1 could be used as a therapeutic niche for patient-tailored therapies PMID: 30201002
  2. The bromodomain and extraterminal domain (BET) family consists of BRDT, BRD2, BRD3, and BRD4, each containing 2 bromodomains located N-terminal to extraterminal domain, which is located near C-terminus. Data suggest that 10 distinct acylations participate in binding of BET family proteins to histone 4 (oligopeptide fragments used here); C-terminal bromodomains do not cooperatively bind multiple acylation sites. PMID: 28945351
  3. The affected patient carries a homozygous bromodomain, testis-specific protein (BRDT) mutation, while the patient's father, mother and elder brother all carry heterozygous BRDT mutations. PMID: 28199965
  4. considered potential associations of 14 single nucleotide polymorphisms (SNPs) in RNF8 and BRDT genes in Chinese patients with non-obstructive azoospermia PMID: 25374327
  5. assessment of rs3088232 frequency in large group of non-obstructive azoospermia men and fertile controls demonstrated no significant difference between them; conclude that testicular impairments observed were not a consequence of BRDT gene mutation PMID: 24865796
  6. In human, BRDT is the only BET gene expressed exclusively in testicular germ cells. PMID: 22035730
  7. Suggest that reduced histone methylation in the promoter of BRDT may be associated with increased transcript levels in subfertile patients. PMID: 20538714
  8. BRDT-NY gene is an alternatively spliced variant that may have an important role in the process of spermatogenesis and may be correlated with male infertility PMID: 15647849

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Involvement in disease Spermatogenic failure 21 (SPGF21)
Subcellular Location Nucleus
Tissue Specificity Testis-specific. A 3-fold higher expression is seen in adult testis than in embryo testis. Expression seems to be correlated with histone H4 hyperacetylation during the haploid phase of spermatogenesis (spermiogenesis). No expression, or very low expressi
Database Links

HGNC: 1105

OMIM: 602144

KEGG: hsa:676

STRING: 9606.ENSP00000354568

UniGene: Hs.482520

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