Human C-type lectin domain family 2 member D(CLEC2D) ELISA kit

Instructions
Code CSB-EL005528HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name C-type lectin domain family 2, member D
Alternative Names C type lectin related f ELISA Kit; C type lectin superfamily 2; member D ELISA Kit; C-type lectin domain family 2 member D ELISA Kit; CLAX ELISA Kit; CLC2D_HUMAN ELISA Kit; CLEC2D ELISA Kit; CLEC2D protein ELISA Kit; Clec2d5 ELISA Kit; L LT1 ELISA Kit; Lectin like NK cell receptor ELISA Kit; Lectin like transcript 1 ELISA Kit; Lectin-like NK cell receptor ELISA Kit; Lectin-like transcript 1 ELISA Kit; LLT-1 ELISA Kit; LLT1 ELISA Kit; OCIL ELISA Kit; Osteoclast inhibitory lectin ELISA Kit
Abbreviation CLEC2D
Uniprot No. Q9UHP7
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %89
Range %85-93
1:2Average %95
Range %92-99
1:4Average %87
Range %83-91
1:8Average %90
Range %84-97
Recovery
The recovery of human CLEC2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-97
EDTA plasma (n=4)9690-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.575 2.600 2.588 2.445
10002.215 2.147 2.181 2.038
5001.533 1.498 1.516 1.373
2500.901 0.944 0.923 0.780
1250.531 0.565 0.548 0.405
62.50.365 0.344 0.355 0.212
31.250.258 0.250 0.254 0.111
00.139 0.147 0.143
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Citations

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Target Background

Function
(From Uniprot)
Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis
Gene References into Functions
  1. These results show that susceptibility of normal articular chondrocytes to lysis by NK cells is modulated by NKR-P1A/LLT1 interactions. Thus, NKR-P1A/LLT1 interaction might provide some novel target for therapeutic interventions in the course of pathological cartilage injury. PMID: 29212911
  2. Blocking LLT1-NKRP1A interaction will make prostate cancer cells susceptible to killing by NK cells, suggesting a therapeutic option for treatment of prostate cancer. PMID: 27626681
  3. these data suggest that LLT1-CD161 interactions play a novel and important role in B cell maturation within the Germinal center in humans. PMID: 26829983
  4. In RA joints, LLT1 is expressed by cells of the monocyte/macrophage lineage. PMID: 26147876
  5. The hexamer of glycosylated LLT1 consists of three classical dimers. The hexameric packing may indicate a possible mode of interaction of C-type lectin-like proteins in the glycosylated form. PMID: 25760607
  6. One polymorphism in LLT1 was found to be associated with our Crohn's Disease population (P<0.034).Our Ulcerative Colitis cohort was not associated with the variation in LLT1 (P=0.33) PMID: 22664939
  7. LLT1 and CD161 have roles in modulating immune responses to pathogens; and interferon-gamma contributes to modulate immune responses PMID: 21930700
  8. Molecular basis for LLT1 protein recognition by human CD161 protein (NKRP1A/KLRB1). PMID: 21572041
  9. Data show that only CLEC2D isoform 1 (LLT1) is expressed on the cell surface. PMID: 20843815
  10. LLT1 used Src-PTK, p38 and ERK signalling pathways, but not PKC, PI3K or calcineurin pathways, to increase production of IFN-gamma by human natural killer cells. PMID: 20415786
  11. LLT1 induces Interferon Type II production by natural killer cells. PMID: 15104121
  12. Data show that osteoclast inhibitory lectin (OCIL) binds a range of physiologically important glycosaminoglycans, and this property may modulate OCIL actions upon other cells [OCIL]. PMID: 15123656
  13. Engagement of CD161 on NK cells with LLT1 expressed on target cells inhibited NK cell-mediated cytotoxicity and IFN-gamma secretion. LLT1/CD161 interaction in the presence of a TCR signal enhanced IFN-gamma production by T cells PMID: 16339512
  14. LLT1 on target cells can inhibit NK cytotoxicity via interactions with CD161. LLT1 activates NFAT-GFP reporter cells expressing a CD3zeta-CD161 chimeric receptor; reciprocally, reporter cells with a CD3zeta-LLT1 chimeric receptor are stimulated by CD161 PMID: 16339513
  15. Expression of LLT1 on activated dendritic cells and B cells suggests that it might regulate the cross-talk between NK cells and APCs PMID: 18453569
  16. Women with a lysine (GG genotype) at position 19 of the OCIL protein displayed lower bone mineral density at femoral neck and at lumbar spine sites than women having an asparagine residue. PMID: 18465072

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Subcellular Location Cell membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Isoform 2: Endoplasmic reticulum, SUBCELLULAR LOCATION: Isoform 4: Endoplasmic reticulum
Tissue Specificity Detected in peripheral blood leukocytes, osteoblasts, lymph node, thymus and spleen. Isoform 1, isoform 2 and isoform 4 are expressed in T- and B-lymphocytes, and at lower levels in NK cells. They are also expressed in B-cell lines and LPS-matured monocyt
Database Links

HGNC: 14351

OMIM: 605659

KEGG: hsa:29121

UniGene: Hs.268326

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