Human C-type lectin domain family 2 member D(CLEC2D) ELISA kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Target Name

C-type lectin domain family 2, member D

Alternative Names

C type lectin related f ELISA Kit; C type lectin superfamily 2; member D ELISA Kit; C-type lectin domain family 2 member D ELISA Kit; CLAX ELISA Kit; CLC2D_HUMAN ELISA Kit; CLEC2D ELISA Kit; CLEC2D protein ELISA Kit; Clec2d5 ELISA Kit; L LT1 ELISA Kit; Lectin like NK cell receptor ELISA Kit; Lectin like transcript 1 ELISA Kit; Lectin-like NK cell receptor ELISA Kit; Lectin-like transcript 1 ELISA Kit; LLT-1 ELISA Kit; LLT1 ELISA Kit; OCIL ELISA Kit; Osteoclast inhibitory lectin ELISA Kit

Abbreviation

CLEC2D

Uniprot No.

Q9UHP7

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	95
	Range %	92-99
1:4	Average %	87
	Range %	83-91
1:8	Average %	90
	Range %	84-97

Recovery

The recovery of human CLEC2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	96	90-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.575	2.600	2.588	2.445
1000	2.215	2.147	2.181	2.038
500	1.533	1.498	1.516	1.373
250	0.901	0.944	0.923	0.780
125	0.531	0.565	0.548	0.405
62.5	0.365	0.344	0.355	0.212
31.25	0.258	0.250	0.254	0.111
0	0.139	0.147	0.143

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human CLEC2D ELISA Kit was designed for the quantitative measurement of Human CLEC2D protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Target Background

Function
(From Uniprot)

Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon-gamma. Binds high molecular weight sulfated glycosaminoglycans.

Gene References into Functions

These results show that susceptibility of normal articular chondrocytes to lysis by NK cells is modulated by NKR-P1A/LLT1 interactions. Thus, NKR-P1A/LLT1 interaction might provide some novel target for therapeutic interventions in the course of pathological cartilage injury. PMID: 29212911
Blocking LLT1-NKRP1A interaction will make prostate cancer cells susceptible to killing by NK cells, suggesting a therapeutic option for treatment of prostate cancer. PMID: 27626681
these data suggest that LLT1-CD161 interactions play a novel and important role in B cell maturation within the Germinal center in humans. PMID: 26829983
In RA joints, LLT1 is expressed by cells of the monocyte/macrophage lineage. PMID: 26147876
The hexamer of glycosylated LLT1 consists of three classical dimers. The hexameric packing may indicate a possible mode of interaction of C-type lectin-like proteins in the glycosylated form. PMID: 25760607
One polymorphism in LLT1 was found to be associated with our Crohn's Disease population (P<0.034).Our Ulcerative Colitis cohort was not associated with the variation in LLT1 (P=0.33) PMID: 22664939
LLT1 and CD161 have roles in modulating immune responses to pathogens; and interferon-gamma contributes to modulate immune responses PMID: 21930700
Molecular basis for LLT1 protein recognition by human CD161 protein (NKRP1A/KLRB1). PMID: 21572041
Data show that only CLEC2D isoform 1 (LLT1) is expressed on the cell surface. PMID: 20843815
LLT1 used Src-PTK, p38 and ERK signalling pathways, but not PKC, PI3K or calcineurin pathways, to increase production of IFN-gamma by human natural killer cells. PMID: 20415786
LLT1 induces Interferon Type II production by natural killer cells. PMID: 15104121
Data show that osteoclast inhibitory lectin (OCIL) binds a range of physiologically important glycosaminoglycans, and this property may modulate OCIL actions upon other cells [OCIL]. PMID: 15123656
Engagement of CD161 on NK cells with LLT1 expressed on target cells inhibited NK cell-mediated cytotoxicity and IFN-gamma secretion. LLT1/CD161 interaction in the presence of a TCR signal enhanced IFN-gamma production by T cells PMID: 16339512
LLT1 on target cells can inhibit NK cytotoxicity via interactions with CD161. LLT1 activates NFAT-GFP reporter cells expressing a CD3zeta-CD161 chimeric receptor; reciprocally, reporter cells with a CD3zeta-LLT1 chimeric receptor are stimulated by CD161 PMID: 16339513
Expression of LLT1 on activated dendritic cells and B cells suggests that it might regulate the cross-talk between NK cells and APCs PMID: 18453569
Women with a lysine (GG genotype) at position 19 of the OCIL protein displayed lower bone mineral density at femoral neck and at lumbar spine sites than women having an asparagine residue. PMID: 18465072

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Subcellular Location

Cell membrane; Single-pass type II membrane protein.; [Isoform 2]: Endoplasmic reticulum.; [Isoform 4]: Endoplasmic reticulum.

Tissue Specificity

Detected in peripheral blood leukocytes, osteoblasts, lymph node, thymus and spleen. Isoform 1, isoform 2 and isoform 4 are expressed in T- and B-lymphocytes, and at lower levels in NK cells. They are also expressed in B-cell lines and LPS-matured monocyt

Database Links

HGNC: 14351

OMIM: 605659

KEGG: hsa:29121

UniGene: Hs.268326

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Code	CSB-EL005528HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.